Archiv für die gesamte Virusforschung

, Volume 46, Issue 1–2, pp 167–174 | Cite as

Pinocytotic uptake and intralysosomal crystal formation of Coxsackievirus A9 in monkey kidney cells

An electron microscopic autoradiographic study
  • W. Hecker
  • J. Meyer
  • Regula Boeni
  • K. Bienz


Infection of monkey kidney cells by Coxsackievirus A 9 labelled with3H-uridine was studied. The penetration process and subsequent localization of viral RNA was examined by electron microscopic autoradiography: Single virions were pinocytozed and aggregated to groups within membrane bound vesicles. The virions were then rearranged to large crystals within these vesicles. At 3 hours after infection most of the crystal-containing vesicles displayed acid phosphatase activity and are therefore considered to be lysosomes.

At this time no progeny virus was detected in the cells. Later in the replicative cycle radioactivity was found in the paranuclear, vacuolated region of cytopathic cells and to a small extent in progeny virus.


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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • W. Hecker
    • 1
  • J. Meyer
    • 1
  • Regula Boeni
    • 1
  • K. Bienz
    • 1
  1. 1.Institut for Microbiology and HygieneUniversity of BaselBaselSwitzerland

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