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Cell and Tissue Research

, Volume 248, Issue 1, pp 207–214 | Cite as

Structural changes at pure cholinergic synaptosomes during the transmitter release induced by A-23187 inTorpedo marmorata

A freeze-fracture study
  • G. Egea
  • J. E. Esquerda
  • R. Calvet
  • C. Solsona
  • J. Marsal
Article

Summary

Pure cholinergic synaptosomes isolated from the electric organ ofTorpedo marmorata were stimulated by calcium ionophore A-23187. The effect of time course of stimulation on the changes in intramembrane particles (IMPs) on presynaptic membranes was studied by quickfreezing and aldehyde-fixation freeze-fracture. We showed that the decrease of small-particle density at the P-face and the increase of large-particle density at the E-face was maximum after 30 sec of A-23187 stimulation. Later, the density of synaptic vesicles decreased. We suggest that the redistribution of IMPs on the presynaptic membrane and acetylcholine (ACh) release from pure cholinergic synaptosomes have a similar time course when triggered by A-23187

Key words

Acetylcholine release Intramembrane particles Synaptic vesicles Calcium ionophore A-23187 Freezefracture Torpedo marmorata 

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • G. Egea
    • 1
  • J. E. Esquerda
    • 1
  • R. Calvet
    • 1
  • C. Solsona
    • 1
  • J. Marsal
    • 1
  1. 1.Departament de Biologia Cellular i Anatomia PatològicaHospital de Bellvitge, Facultat de Medicina, Universitat de BarcelonaBarcelonaSpain

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