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Diabetologia

, Volume 9, Issue 5, pp 400–402 | Cite as

Hyperglucagonemia of the isolated perfused pancreas of diabetic mice (db/db)

  • H. Laube
  • R. D. Fussgänger
  • V. Maier
  • E. F. Pfeiffer
Originals

Summary

Diabetes mellitus is held to be accompanied by inappropriately high levels of plasma glucagon relative to blood glucose concentrations. This has been interpreted as indicating lack of insulin. To establish glucagon release in presence of high levels of endogenous insulin, the effects of both glucose and arginine were studied in the isolated perfused pancreas of genetically diabetic mice (db/db). Stimulation with glucose 2.75 mM or glucose plus arginine 8.25 mM exhibited a pronounced hyperglucagonemia. Following glucose 8.25 mM, however, there was no depression of glucagon secretion. Despite excessive high levels of endogenous insulin, there was a pattern of rather non-suppressible glucagon release. Lack of insulin per se, therefore, is unlikely to be the sole cause of hyperglucagonemia in this type of genetic animal diabetes mellitus.

Key words

Diabetic mice isolated perfused pancreas high insulin levels hyperglucagonemia 

References

  1. 1.
    Assan, R., Rosselin, G., Dolais, J.: Effets sur la glucagonémie des perfusions et ingestions d'acides aminées. J. Ann. Diabètol. Hôtel Dieu7, 25 (1967)Google Scholar
  2. 2.
    Bagdade, J. D., Bierman, E. L., Porte, D. A.: The significance of basal insulin levels in the evaluation of the insulin response to glucose in diabetic subjects. J. clin. Invest.46, 1549 (1967)Google Scholar
  3. 3.
    Cameron, D.P., Amherdt, M., Orci, L., Peyer, R., Stauffacher, W.: Biochemical and morphological studies of immunoreactive insulin secretion in spontaneous and acquired obesity and /or hyperglycemia in rodents. Acta diabet. lat.9, (suppl. 1), 89 (1972)Google Scholar
  4. 4.
    Chick, W.L., Like, A.A.: Studies in the diabetic mutant mouse III: Physiological factors associated with alterations in beta cell proliferation. Diabetologia6, 243 (1970)Google Scholar
  5. 5.
    Coleman, D.L., Hummel, K.P.: The effects of hypothalamic lesions in genetically diabetic mice. Diabetologia6, 263 (1970)Google Scholar
  6. 6.
    Dorner, M., Stahl, J.: Recherches sur le glucagon II. Relation entre l'hypophyse antérieur et le système alpha-cellulaire du pancréas chez le chien. Rev. franç. Étud. clin. biol.2, 118 (1957)Google Scholar
  7. 7.
    Ferner, H.: Das Inselsystem des Pankreas. Stuttgart: Georg Thieme 1952Google Scholar
  8. 8.
    Ferner, H., Tonutti, E.: Die Wirkung der Hypophysektomie auf das Zellbild der Inseln bei Ratten und Meerschweinchen. Z. Zellforsch.38, 267 (1953)Google Scholar
  9. 9.
    Fussgänger, R.D.: Die Rolle des Glukagons bei der Zuckerkrankheit. Dtsch. Med. J.:23, 601 (1972)Google Scholar
  10. 10.
    Fussgänger, R.D., Schröder, K.E., Goberna, R., Pfeiffer, E.F.: Comparative studies on the mechanism of interrelated insulin and glucagon secretion. In prep. (1973)Google Scholar
  11. 11.
    Herbert, V.K., Lau, S., Gottlieb, C.W., Bleicher, S.J.: Coated charcoal immunoassay of insulin. J. clin. Endocr.25, 1375 (1965)Google Scholar
  12. 12.
    Kalkhoff, R., Matute, M., Gossain, V.: Plasma glucagon in obesity. Clin. Res.20, 802 (1972)Google Scholar
  13. 13.
    Katsilambros, N.Y., Abdel Rahman, Hinz, M., Fussgänger, R.D., Schröder, K.E., Straub, K., Pfeiffer, E.F.: Action of streptozotocin on insulin and glucagon responses of rat islets. Horm. Metab. Res.2, 268 (1970)Google Scholar
  14. 14.
    Mayer, J., Andrus, S.B., Silides, D.J.: Effect of diethyldithiocarbamate and other agents on mice with obese-hyperglycemic syndrom. Endocrinology53, 572 (1953)Google Scholar
  15. 15.
    Melani, F., Ditschuneit, H., Bartelt, K., Friedrich, H., Pfeiffer, E.F.: Über die radioimmunologische Bestimmung von Insulin im Blut. Klin. Wschr.43, 1000 (1965)Google Scholar
  16. 16.
    Müller, W.A., Faloona, G.R., Aguilar-Parada, E., Unger, R.H.: Abnormal alpha cell function in diabetes. Response to carbohydrate and protein ingestion. New Engl. J. Med.283, 109 (1970)Google Scholar
  17. 17.
    Müller, W.A., Faloona, G.R., Unger, R.H.: Effect of experimental insulin deficiency on glucagon secretion. J. clin. Invest.50, 1992 (1971)Google Scholar
  18. 18.
    Orci, L., Junod, A., Pictet, R., Renold, A., Rouiller, C.: Granulolysis in a cells of endocrine pancreas in spontaneous and experimental diabetes. J. Cell Biol.38, 462 (1968)Google Scholar
  19. 19.
    Paulsen, E.P., Lawrence, A.M.: Glucagon hypersecretion in obese children. Lancet1968 II, 110Google Scholar
  20. 20.
    Perley, M., Kipnis, D.M.: Plasma insulin response to glucose and tolbutamide of normal weight and obese diabetic subjects. Diabetes15, 867 (1966)Google Scholar
  21. 21.
    Samols, E., Tyler, J.M., Marks, V.: Glucagon-insulin interrelationship. In: Glucagon, p. 151. Edited by Lefebvre, P.L. and Unger, R.H. Oxford: Pergamon Press 1972Google Scholar
  22. 22.
    Schatz, H., Hinz, M., V., Nierle, Chr., Pfeiffer, E.F.: Stimulation of 3H-Leucine incorporation into the proinsulin and insulin fraction of isolated pancreatic mouse islets. Diabetes, submitted and accepted 1973Google Scholar
  23. 23.
    Sussman, K.E., Vaughan, G.D., Timmer, R.F.: An in vitro method for studying insulin secretion in the perfused isolated rat pancreas. Metabolism15, 466 (1966)Google Scholar
  24. 24.
    Unger, R.H.: Glucagon and the insulin: glucagon ratio in diabetes and other catabolic illnesses. Diabetes20, 834 (1971)Google Scholar
  25. 25.
    Unger, R.H., Aguilar-Parada, E., Müller, W.A., Eisentraut, A.M.: Studies of pancreatic alpha cell function in normal and diabetic subjects. J. clin. Invest.49, 837 (1970)Google Scholar
  26. 26.
    Unger, R.H., Madison, L.L., Müller, W.A.: Abnormal alpha cell function in diabetics. Diabetes21, 301 (1972)Google Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • H. Laube
    • 1
  • R. D. Fussgänger
    • 1
  • V. Maier
    • 1
  • E. F. Pfeiffer
    • 1
  1. 1.Department of Endocrinology and Metabolism, Centre of Internal Medicine and PediatricsUniversity of Ulm/D.Germany

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