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Somatic Cell and Molecular Genetics

, Volume 19, Issue 5, pp 431–437 | Cite as

Genetic diversity of mitomycin C-hypersensitive Chinese hamster cell mutants: A new complementation group with chromosomal instability

  • W. J. I. Overkamp
  • M. A. Rooimans
  • I. Neuteboom
  • P. Telleman
  • F. Arwert
  • M. Z. Zdzienicka
Article

Abstract

A Chinese hamster cell mutant (V-C8) isolated previously, which is ∼100 fold more sensitive to mitomycin C (MMC) than its parental wild-type V79 cells (judged by D10 values), was further characterized. V-C8 cells exhibit an increased sensitivity towards other cross-linking agents, such ascis-DDP (∼40-fold), DEB (∼30-fold), and also to adriamycin (∼5-fold), and the monofunctional alkylating agents: MMS (∼5-fold) and EMS (∼6-fold). V-C8 cells show a higher level induction of chromosomal aberrations by cross-linking agents (MMC,cis-DDP, and DEB) and an increased level of spontaneous chromosomal aberrations in comparison to the wild-type V79 cells. To determine whether the V-C8 mutant represents a new complementation group among Chinese hamster cell mutants that also display the extreme sensitivity to MMC, V-C8 cells were fused withirs1, irs1SF, UV20, UV41, and V-H4 cells. In all cases, the derived hybrids regained the MMC sensitivity similar to wild-type cells, indicating that the V-C8 mutant belongs to a new sixth complementation group.

Keywords

Genetic Diversity Mitomycin Adriamycin Chromosomal Aberration Cell Mutant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1993

Authors and Affiliations

  • W. J. I. Overkamp
    • 1
  • M. A. Rooimans
    • 2
  • I. Neuteboom
    • 1
    • 3
  • P. Telleman
    • 1
    • 2
  • F. Arwert
    • 2
  • M. Z. Zdzienicka
    • 1
    • 3
  1. 1.MGC-Department of Radiation Genetics and Chemical MutagenesisUniversity of LeidenAL LeidenThe Netherlands
  2. 2.Department of AnthropogeneticsFree UniversityAmsterdamThe Netherlands
  3. 3.J.A. Cohen InstituteInteruniversity Research Institute for Radiopathology and Radiation ProtectionLeidenThe Netherlands

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