Somatic Cell and Molecular Genetics

, Volume 19, Issue 5, pp 431–437 | Cite as

Genetic diversity of mitomycin C-hypersensitive Chinese hamster cell mutants: A new complementation group with chromosomal instability

  • W. J. I. Overkamp
  • M. A. Rooimans
  • I. Neuteboom
  • P. Telleman
  • F. Arwert
  • M. Z. Zdzienicka


A Chinese hamster cell mutant (V-C8) isolated previously, which is ∼100 fold more sensitive to mitomycin C (MMC) than its parental wild-type V79 cells (judged by D10 values), was further characterized. V-C8 cells exhibit an increased sensitivity towards other cross-linking agents, such ascis-DDP (∼40-fold), DEB (∼30-fold), and also to adriamycin (∼5-fold), and the monofunctional alkylating agents: MMS (∼5-fold) and EMS (∼6-fold). V-C8 cells show a higher level induction of chromosomal aberrations by cross-linking agents (MMC,cis-DDP, and DEB) and an increased level of spontaneous chromosomal aberrations in comparison to the wild-type V79 cells. To determine whether the V-C8 mutant represents a new complementation group among Chinese hamster cell mutants that also display the extreme sensitivity to MMC, V-C8 cells were fused withirs1, irs1SF, UV20, UV41, and V-H4 cells. In all cases, the derived hybrids regained the MMC sensitivity similar to wild-type cells, indicating that the V-C8 mutant belongs to a new sixth complementation group.


Genetic Diversity Mitomycin Adriamycin Chromosomal Aberration Cell Mutant 
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Literature cited

  1. 1.
    Smith, C.A. (1988). InPsoralen DNA Photobiology, vol. 2, (ed.) Gasparro, F. (CRC Press, Boca Raton, Florida) pp. 47–116.Google Scholar
  2. 2.
    Sasaki, M.S., and Tonomura, A. (1973).Cancer Res. 331829–1836.PubMedGoogle Scholar
  3. 3.
    Strathdee, C.A., Duncan, A.M.V., and Buchwald, M. (1992).Nature Genet. 1196–198.PubMedGoogle Scholar
  4. 4.
    Burns, M.A., and Tomkins, D.J. (1989).Mutat. Res. 216243–249.PubMedGoogle Scholar
  5. 5.
    Akamatsu, N., Mijaki, M., Suzuki, K., Ono, T., and Sasaki, M.S. (1983).Mutat. Res. 120173–180.PubMedGoogle Scholar
  6. 6.
    Sasaki, M.S. (1978). InDNA Repair Mechanisms, (eds.) Hanawalt, P.C., Friedberg, E.C., and Fox, C.F. (Academic Press, New York), pp. 675–684.Google Scholar
  7. 7.
    Nishisho, I., Nakamaura, Y., Miyoshi, Y., Miki, Y., Ando, H., Horii, A., Koyama, K., Utsunomiya, J., Baba, S., Hedge, P., Markham, A., Krush, A.J., Petersen, G., Hamilton, S.R., Nilbert, M.C., Levy, D.B., Bryan, T.M., Preisinger, A.C., Smith, K.J., Su, L.-K., Kinzler K.W., and Vogelstein, B. (1991).Science 253665–669.PubMedGoogle Scholar
  8. 8.
    Hama-Inaba, H., Hieda-Shiomi, N., Shiomi, T., and Sato, K. (1983).Mutat. Res. 108405–416.PubMedGoogle Scholar
  9. 9.
    Robson, C.N., Harris, A.L., and Hickson, I.D. (1985).Cancer Res. 455304–5309.PubMedGoogle Scholar
  10. 10.
    Zdzienicka, M.Z., and Simons, J.W.I.M. (1987).Mutat. Res. 178235–244.PubMedGoogle Scholar
  11. 11.
    Hoy, C.A., Thompson, L.H., Mooney, C.L., and Salazar, E.P. (1985).Cancer Res. 451737–1743.PubMedGoogle Scholar
  12. 12.
    Jones, H.J., Cox, R., and Thacker, J. (1987).Mutat. Res. 183279–286.PubMedGoogle Scholar
  13. 13.
    Jones, N.J., Stewart, S.A., and Thompson, L.H. (1989).Mutagenesis 515–23.Google Scholar
  14. 14.
    Zdzienicka, M.Z., Arwert, F., Neuteboom, I., Rooimans, M., and Simons, J.W.I.M. (1990).Somat. Cell Mol. Genet. 16575–581.PubMedGoogle Scholar
  15. 15.
    Standing Committee on Human Cytogenetic Nomenclature. (1978).Cytogenet. Cell Genet. 21363–374.Google Scholar
  16. 16.
    Gianni, L., Corden, B.J., and Myers, C. (1983).Rev. Biochem. Toxicol. 51–82.Google Scholar
  17. 17.
    Arwert, F., Rooimans, M., Westerveld, A., Simons, J.W.I.M., and Zdzienicka, M.Z. (1991).Cytogenet. Cell Genet. 5623–26.PubMedGoogle Scholar
  18. 18.
    Tucker, J.D., Jones, N.J., Allen, A.N., Minkler, J.L., Thompson, L.H., and Carrano, A.V. (1991).Mutat. Res. 254143–152.PubMedGoogle Scholar
  19. 19.
    Poll, E.H.A., Arwert, F., Joenje, H., and Erickson, A.W. (1982).Hum. Genet. 61228–230.PubMedGoogle Scholar
  20. 20.
    Ishida, R., and Buchwald, M. (1982).Cancer Res. 424000–4006.PubMedGoogle Scholar
  21. 21.
    Schröder, T.M. (1982).Cytogenet. Cell Genet. 33119–132.PubMedGoogle Scholar
  22. 22.
    Chaganti, R.S.K., Schonberg, S., and German, J. (1974).Proc. Natl. Acad. Sci. U.S.A. 714508–4512.PubMedGoogle Scholar
  23. 23.
    Hoeijmakers, J.H.J., Odijk, H., and Westerveld, A. (1987).Exp. Cell. Res. 169111–119.PubMedGoogle Scholar
  24. 24.
    Westerveld, A., Hoeijmakers, J.H.J., Duin, M. van, Wit, J. de, Odijk, H., Pastink, A., Wood, R.D., and Bootsma, D. (1984).Nature 31425–429.Google Scholar
  25. 25.
    Weber, C.A., Salazar, E.P., Stewart, S.A., and Thompson, L.H. (1988).Mol. Cell. Biol. 81137–1146.PubMedGoogle Scholar
  26. 26.
    Weeda, G., Ham, C.A.R. van, Masurel, R., Westerveld, A., Odijk, H., Wit, J. de, Bootsma, D., Eb, A.J. van der, and Hoeijmakers, J.H.J. (1990).Mol. Cell. Biol. 102570–2581.PubMedGoogle Scholar
  27. 27.
    Thompson, L.H., Brookman, K.W., Jones, N.J., Allen, S.A., and Carrano, A.V. (1990).Mol. Cell. Biol. 106160–6171.PubMedGoogle Scholar
  28. 28.
    Troelstra, C., van Gool, A., de Wit, J., Vermeulen, W., Bootsma, D., and Hoeijmakers, J.H.J. (1992).Cell 71939–9539.PubMedGoogle Scholar
  29. 29.
    Thompson, L.H., Rubin, J.S., Cleaver, J.E., Whitmore, G.F., and Brookman, K. (1980).Somat. Cell Genet. 6391–405.PubMedGoogle Scholar
  30. 30.
    Sorenson, C.M., and Eastman, E. (1988).Cancer Res. 186703–6707.Google Scholar
  31. 31.
    Caldecott, K., and Jeggo, P. (1991).Mutat. Res. 255111–121.PubMedGoogle Scholar
  32. 32.
    Fuller, L.F., and Painter, R.B. (1988).Mutat. Res. 193109–121.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1993

Authors and Affiliations

  • W. J. I. Overkamp
    • 1
  • M. A. Rooimans
    • 2
  • I. Neuteboom
    • 1
    • 3
  • P. Telleman
    • 1
    • 2
  • F. Arwert
    • 2
  • M. Z. Zdzienicka
    • 1
    • 3
  1. 1.MGC-Department of Radiation Genetics and Chemical MutagenesisUniversity of LeidenAL LeidenThe Netherlands
  2. 2.Department of AnthropogeneticsFree UniversityAmsterdamThe Netherlands
  3. 3.J.A. Cohen InstituteInteruniversity Research Institute for Radiopathology and Radiation ProtectionLeidenThe Netherlands

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