Somatic Cell and Molecular Genetics

, Volume 16, Issue 5, pp 501–505

Localization of gene for human syndecan, an integral membrane proteoglycan and a matrix receptor, to chromosome 2

  • M. Ala-Kapee
  • H. Nevanlinna
  • M. Mali
  • M. Jalkanen
  • J. Schröder
Brief Communication

Abstract

Syndecan, a cell surface proteoglycan, is an integral membrane protein acting as a receptor for the extracellular matrix. For chromosomal localization of the human syndecan gene, a panel of mouse-human somatic cell hybrids was analyzed by Southern blotting using the cDNA probe for human syndecan. The hybrids were karyotyped at the time of DNA extraction. A band corresponding to the human syndecan gene in Southern blots was found only in a hybrid cell line containing human chromosome 2. This hybrid was subcloned and its subclones were analyzed by Southern blotting and karyotyped. Subclones carrying human chromosome 2 contained the syndecan gene, while subclones not carrying this chromosome did not. The human syndecan gene is thus assigned to chromosome 2.

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Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • M. Ala-Kapee
    • 1
  • H. Nevanlinna
    • 2
  • M. Mali
    • 3
  • M. Jalkanen
    • 3
  • J. Schröder
    • 1
  1. 1.Department of GeneticsUniversity of HelsinkiHelsinkiFinland
  2. 2.Department of Obstetrics and GynecologyUniversity of HelsinkiHelsinkiFinland
  3. 3.Department of Medical BiochemistryUniversity of TurkuTurkuFinland

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