Somatic Cell and Molecular Genetics

, Volume 16, Issue 5, pp 501–505 | Cite as

Localization of gene for human syndecan, an integral membrane proteoglycan and a matrix receptor, to chromosome 2

  • M. Ala-Kapee
  • H. Nevanlinna
  • M. Mali
  • M. Jalkanen
  • J. Schröder
Brief Communication


Syndecan, a cell surface proteoglycan, is an integral membrane protein acting as a receptor for the extracellular matrix. For chromosomal localization of the human syndecan gene, a panel of mouse-human somatic cell hybrids was analyzed by Southern blotting using the cDNA probe for human syndecan. The hybrids were karyotyped at the time of DNA extraction. A band corresponding to the human syndecan gene in Southern blots was found only in a hybrid cell line containing human chromosome 2. This hybrid was subcloned and its subclones were analyzed by Southern blotting and karyotyped. Subclones carrying human chromosome 2 contained the syndecan gene, while subclones not carrying this chromosome did not. The human syndecan gene is thus assigned to chromosome 2.


Extracellular Matrix Membrane Protein Somatic Cell Southern Blotting Human Chromosome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • M. Ala-Kapee
    • 1
  • H. Nevanlinna
    • 2
  • M. Mali
    • 3
  • M. Jalkanen
    • 3
  • J. Schröder
    • 1
  1. 1.Department of GeneticsUniversity of HelsinkiHelsinkiFinland
  2. 2.Department of Obstetrics and GynecologyUniversity of HelsinkiHelsinkiFinland
  3. 3.Department of Medical BiochemistryUniversity of TurkuTurkuFinland

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