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Diabetologia

, Volume 3, Issue 2, pp 212–221 | Cite as

Studies on a diabetic (KK) strain of the mouse

  • Mitsuo Nakamura
  • Kazuyori Yamada
Article

Summary

This review demonstrates the following points:1. By glucose tolerance test and the determination of non-fasting blood sugar values, mice of KK strain (a Japanese strain) have been shown to be in a diabetic state. 2. KK mice have greater than normal body weights, and moderate obesity. 3. Pancreatic insulin activity and adenohypophyseal growth hormone content of KK mice are greater than those of C75BL mice. 4. The pancreas of KK mice presents many striking changes, such as the hypertrophy and hyperplasia of the islets, the hypertrophy and degranulation of B cells, the abundance of B cell ribosomes and endoplasmic reticulum, the enlargement of the Golgi areas of B cells, and the diminution of the zinc content of insular cells. 5. In other organs (eg. adenohypophysis, liver, adrenal and parathyroid) of KK mice, many changes are also observed. The above features of KK mice are discussed in relation to the presumed metabolic disorder in the mice.

Key-words

Spontaneous Diabetes KK mice Japanese mice Obesity Growth hormone Pituitary Islets of Langerhans Pancreas Ultrastructure Beta cells Insulin in pancreas Sex and diabetes Adrenal cortex Zinc 

Etudes d'une souche diabétique (KK) de souris

Résumé

1. Un état diabétique est démontré chez des souris d'une souche japonaise, la souche KK, mis en évidence par une élévation du sucre sanguin et par une diminution de la tolérance au glucose. 2. Le poids corporel des souris KK dépasse la normale; il s'agit d'une obésité modérée. 3. L'activité insulinique du pancréas et le contenu en hormone de croissance de l'hypophyse de souris KK dépassent ceux observés chez des souris C57BL. 4. Histologiquement, on trouve chez les souris KK une hypertrophie et une hyperplasie des îlots de Langerhans, une hypertrophie et une dégranulation des cellules B, une abondance de ribosomes et de réticulum endoplasmique des cellules B, un développement marqué de la région de Golgi des mêmes cellules, et une diminution du contenu en zinc des cellules insulaires. D'autres anomalies ont également été observées dans d'autres organes des souris KK, plus particulièrement pour l'hypophyse, le foie, les surrénales et la parathyroïde.

Untersuchungen beim diabetischen (KK) Mäusestamm

Zusammenfassung

1. Bei Mäusen des KK-Stammes (japanische Zucht) ist mit Bestimmung von Glucosetoleranz und Blutzuckerwerten ein diabetischer Zustand nachweisbar. 2. Das Körpergewicht von KK-Mäusen liegt über der Norm, und es besteht eine leichte Fettsucht. 3. Die Insulinaktivität des Pankreas und der Gehalt der Hypophyse an Wachstumshormon sind bei KK-Mäusen höher als bei C57BL-Mäusen. 4. Das Pankreas der KK-Mäuse zeigt auffallende Veränderungen, wie Hypertrophie und Degranulierung derβ-Zellen, reichlich Ribosomen und endoplasmatisches Reticulum in denβ-Zellen, sowie starke Ausbildung des Golgiapparates und Verminderung des Zinkgehaltes der Inselzellen. 5. Auch andere Organe (z.B. Adenohypophyse, Leber, Nebenniere und Nebenschilddrüse) der KK-Mäuse weisen Veränderungen auf.

References

  1. Barrnett, R., R. Marshall andA. Seligman: Histochemical demonstration of insulin in the islet of Langerhans. Endocrinology57, 419–438 (1955).Google Scholar
  2. Best, C., C. Jephcott andD. Scott: Insulin in tissue other than the pancreas. Amer. J. Physiol.100, 285–295 (1932).Google Scholar
  3. Bielschowsky, M., andF. Bielschowsky: The New Zealand strain of obese mice. Their response to stilboestrol and to insulin. Aust. J. exp. Biol. med. Sci.34, 181–198 (1956).Google Scholar
  4. Bjorkman, N., C. Hellerström andB. Hellman: The ultrastructure of the islets of Langerhans in normal and obese-hyperglycemic mice. Z. Zellforsch.,58, 803–819 (1963).Google Scholar
  5. Elftman, H.: A chrom-alum fixative for the pituitary. Stain Technol.32, 25–28 (1957).Google Scholar
  6. Gomori, G.: The distribution of phosphatase in normal organs and tissues. J. cell. comp. Physiol.17, 71–83 (1941).Google Scholar
  7. — Observations with differential stains on human islet of Langerhans. Amer. J. Path.17, 395–406 (1941).Google Scholar
  8. — An improved histochemical technic for acid phosphatase. Stain Technol.25, 81, (1950).Google Scholar
  9. Hellerström, C., andB. Hellman: The islets of Langerhans in yellow obese mice. Metabolism12, 527–536 (1963).Google Scholar
  10. Jones, E.: Spontaneous hyperplasia of the pancreatic islets associated with glucosuria in hybrid mice. The Structure and Metabolism of the Pancreatic Islets, edited byBrolin S.E., B. Hellman andH. Knutson, Oxford, Pergamon Press, pp. 189–193, 1964.Google Scholar
  11. Kondo, K., K. Nozawa, T. Tomida andK. Ezaki: Inbred strains resulting from Japanese mice. Bull. exp. Animals6, 107–112 (1957).Google Scholar
  12. Lazarow, A., C. Bauer andA. Lindall: Protein synthesis in islet tissue. The Structure and Metabolism of the Pancreatic Islets, edited byBrolin, S.E., B. Hellman andH. Knutson. Oxford, Pergamon Press, pp. 203–221, 1964.Google Scholar
  13. Lazarus, S.: Demonstration of glucose-6-phosphatase in mammalian pancreas. Proc. Soc. exp. Biol.101, 819–822 (1959).Google Scholar
  14. Lewis, U.J., E.V. Cheever andW.P. Vanderlaan:Studies on the growth hormone of normal and dwarf mice. Endocrinology76, 210–215 (1965).Google Scholar
  15. Like, A., J. Steinke E. Jones andG. Cahiil: Pancreatic studies in mice with spontaneous diabetes. Amer. J. Path.46, 621–644 (1965).Google Scholar
  16. Maeda, T., T. Fujiwara andK. Hironari: Histochemical demonstration of zinc. Bull. Kobe Med. College5, 97–99 (1954).Google Scholar
  17. Nakamura, M.: A diabetic strain of the mouse. Proc. Japan. Acad.38, 348–352 (1962).Google Scholar
  18. — Cytological and histological studies on the pancreatic islets of a diabetic strain of the mouse. Z. Zellforsch.65, 340–349 (1965).Google Scholar
  19. — Estimation of adenohypophyseal growth hormone content in the diabetic “KK” mouse strain by acrylamide gel electrophoresis. Proc. Japan. Acad.42, 512–516 (1966).Google Scholar
  20. —, andK. Yamada, Jr.: A further study of the diabetic (KK) strain of the mouse. F1 and F2 offsprings of the cross between KK and C57BL mice. Proc. Japan. Acad.39, 489–493 (1963).Google Scholar
  21. — — Enzymorphological studies on the pancreatic islets of a diabetic (KK) strain of the mouse. Z. Zellforsch.66, 396–404 (1965).Google Scholar
  22. Ogita, Z.: Disc electrophoresis. Metabolism and Disease2, 419–435 (1965).Google Scholar
  23. Padykula, H., andE. Herman: Factors affecting activity of adenosinetriphosphatase and other phosphatases as measured by histochemical techniques. J. Histochem. Cytochem.3, 161–169 (1955a).Google Scholar
  24. — — Specificity of histochemical method for adenosinetriphosphatase. J. Histochem. Cytochem.3, 170–195 (1955b).Google Scholar
  25. Palade, G.: A study of fixation for electron microscopy. J. exp. Med.95, 285–298 (1952).Google Scholar
  26. Tsuchida, I.: Studies on the mechanism of hyperglycemia and obesity in KK strain of the mice. Japan Diabetic Soc.9, 67–84 (1966).Google Scholar
  27. Wachstein, M.: Histochemical staining reactions of the normally functioning and abnormal kidney. J. Histochem. Cytochem.3, 246–270 (1956).Google Scholar
  28. Wrenshall, G., S. Andrus andJ. Mayer: High levels of pancreatic insulin coexistent with hyperglycemia and degranulation of beta cells in mice with the hereditary obese-hyperglycemic syndrome. Endocrinology56, 335–340 (1954).Google Scholar
  29. Yamada, K., Jr.: The chemical cytology of the mouse parathyroid gland. Z. Zellforsch.65, 805–813 (1965).Google Scholar
  30. - M.Nakamura and K.Yamashita: Light and electron microscopic studies on the adenohypophysis of a Diabetic (KK) strain of the mouse. In press (1966).Google Scholar

Copyright information

© Springer-Verlag 1967

Authors and Affiliations

  • Mitsuo Nakamura
    • 1
  • Kazuyori Yamada
    • 1
  1. 1.Department of AnatomyNagoya University School of MedicineJapan

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