Diabetologia

, Volume 11, Issue 6, pp 495–500

Influence of anoxia on glucose metabolism in pancreatic islets: Lack of correlation between fructose-1,6-diphosphate and apparent glycolytic flux

  • B. Hellman
  • L. -Å. Idahl
  • J. Sehlin
  • I. -B. Täljedal
Originals

Summary

When equilibrated with O2-CO2 (95:5), pancreatic islets of non-inbredob/ob-mice exhibited a sigmoidal dependence of3H2O production on D-(5-3H)-glucose concentration; the rate was most sensitive to changes of glucose concentration around 5mM and tended to be maximum above about 15 mM glucose.3H2O production from more than 5 mM D-(5-3H)-glucose was about twice as fast as the production of14CO2 from equimolar D-(U-14C)-glucose. Islets equilibrated with N2-CO2 (95:5) did not exhibit a sigmoidal dose-response curve for3H2O production, the process being inhibited by anoxia at glucose concentrations above 5mM. Pieces of exocrine pancreas had a slower aerobic3H2O production than the islets and showed a clear enhancement of the process during anoxia. In comparison with oxygenated islets, anoxic islets exhibited decreased concentrations of glucose-6-phosphate and increased concentrations of fructose-l,6-diphosphate. The concomitant inhibition of glycolytic flux may be due to a low lactate dehydrogenase activity in islets yielding a slow reoxidation of NADH and a slow phosphoglyceraldehyde oxidation under anaerobic conditions.

Key words

Anoxia glucose metabolism pancreatic islets Pasteur effect 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • B. Hellman
    • 1
  • L. -Å. Idahl
    • 1
  • J. Sehlin
    • 1
  • I. -B. Täljedal
    • 1
  1. 1.Dept. of HistologyUniv. of UmeåUmeåSweden

Personalised recommendations