, Volume 10, Supplement 1, pp 559–565 | Cite as

Effect of diet limitation on the development of diabetes in prediabetic Chinese hamsters

  • G. C. Gerritsen
  • M. C. Blanks
  • R. L. Miller
  • W. E. Dulin


Prediabetic Chinese hamsters born of two ketonuric diabetic parents were hyperphagic from birth. Carcass lipids and total solids were increased but plasma and pancreatic insulin were not, suggesting that hyperphagia was not due to hyperinsulinism. Hyperphagia was controlled by diet limitation of prediabetic pups. Diet limitation for the weaning period only did not alter development of diabetes, but diet limitation for the first 150 days significantly reduced onset and severity. These 150 day diet-limited prediabetics were switched to nonrestricted feeding and subsequently developed mild diabetes. Prediabetic siblings, fedad libitum, developed glucosuria and ketonuria, and died prematurely compared with diet-limited siblings. Prediabetics limited to a normal food intake for 30 months have remained essentially clinically normal. The data strongly suggest that appetite control mechanisms are abnormal prior to clinical signs of diabetes in the prediabetic Chinese hamster and that control of hyperphagia will retard anameliorate the course of diabetes.

Key words

Chinese hamster prediabetes diabetes diet-limitation diabetes prevention diabetes ameliorati on, and increased longevity. 


  1. 1.
    Gerritsen, G.C., Blanks, M.C.: Preliminary studies on food and water consumption of prediabetic Chinese hamsters. Diabetologia6, 177–179 (1970)PubMedGoogle Scholar
  2. 2.
    Gerritsen, G.C., Chang, A.Y., Schneider, D.I., Needham, L.B., Blanks, M.C.: Studies on prediabetic Chinese hamsters. Diabetes.20, 356 (1971)Google Scholar
  3. 3.
    Gerritsen, G.C., Dulin, W.E.: Effect of diet restriction on onset of development of diabetes in prediabetic Chinese hamsters. Acta diabet. lat.9 (Suppl.), 597–613 (1972)Google Scholar
  4. 4.
    Bouchardat, H.: De La Glucosurie du Diabète Sucré. Paris 1883Google Scholar
  5. 5.
    Pyke, D.A.: In: Clinical diabetes and its biochemical basis (Oakley, W.G., Pyke, D.A., Taylor, K.W., eds.) p. 245. London: Blackwell 1968Google Scholar
  6. 6.
    Gerritsen, G.C., Needham, L.B., Schmidt, F.L., Dulin, W.E.: Studies on the prediction and development of diabetes in offspring of diabetic Chinese hamsters. Diabetologia6, 158–162 (1970)PubMedGoogle Scholar
  7. 7.
    Dulin, W.E., Gerritsen, G.C.: Interaction of genetics and environment on diabetes in the Chinese hamster as compared with human and other diabetic animal species. Acta diabet. lat.9 (Suppl. 1), 48–84 (1972)Google Scholar
  8. 8.
    Gerritsen, G.C., Dulin, W.E.: Characterization of diabetes in the Chinese hamster. Diabetologia3, 74–84 (1967)PubMedGoogle Scholar
  9. 9.
    Blanks, M.C., Gerritsen, G.C.: An ultramicro assay for insulin. Proc. Soc. exp. Biol. (N.Y.)146, 448–452 (1974)Google Scholar
  10. 10.
    Zaharko, D.S., Beck, L.V.: Studies of a simplified insulin immunoassay using cellulose powder. Diabetes17, 444–457 (1968)PubMedGoogle Scholar
  11. 11.
    Folch, J., Lees, M., Sloan Stanley, G.H.: A simple method for the isolation and purification of total lipids from animal tissues. J. biol. Chem.226, 497–509 (1957)PubMedGoogle Scholar
  12. 12.
    Natelson, S.: Microtechniques of clinical chemistry, p. 101. Springfield, Ill.: C.C. Thomas 1961Google Scholar
  13. 13.
    Snell, C.T., Snell, F.D.: Colorimetric methods of analysis, p. 184. Toronto: D. van Nostrand 1945Google Scholar
  14. 14.
    Gerritsen, G.C., Dulin, W.E.: Serum proteins of Chinese hamsters and response of diabetics to tolbutamide and insulin. Diabetes15, 331–335 (1966)PubMedGoogle Scholar
  15. 15.
    Schmidt, F.L., Leslie, L.G., Schultz, J.R., Gerritsen, G.C.: Epidemiological studies of the Chinese hamster. Diabetologia6, 154–157 (1970)PubMedGoogle Scholar
  16. 16.
    May, K.K., Beaton, J.R.: Hyperphagia in the insulin-treated rat. Proc. Soc. exp. Biol. (N.Y.)127, 1201–1204 (1968)Google Scholar
  17. 17.
    Oakley, N.W., Beard, R.W., Turner, R.C.: Effect of sustained maternal hyperglycaemia on the fetus in normal and diabetic pregnancies. Brit. med. J. 1972I, 466–469Google Scholar
  18. 18.
    Krauer, F., Joyce, J., Young, M.: The influence of high maternal plasma gucose levels and maternal blood flow on the placental transfer of glucose in the guinea pig. Diabetologia9, 453–456 (1973)PubMedGoogle Scholar
  19. 19.
    Farquhar, J.W.: The significance of hypoglycemia in the newborn infant of the diabetic woman. Arch. Dis. Childh.31, 203–211 (1956)PubMedGoogle Scholar
  20. 20.
    Cornblath, M., Joassin, G., Weisskopf, B., Swiatek, K.R.: Hypoglycemia in the newborn. Pediat. Clin. N. Amer.13, 905–920 (1966)PubMedGoogle Scholar
  21. 21.
    Gerritsen, G.C., Johnson, M. A., Schultz, J.R., Soret, M.G.: Epidemiology of Chinese hamsters and preliminary evidence for genetic heterogeneity of diabetes. Diabetologia10, 581–588 (1974)PubMedGoogle Scholar
  22. 22.
    Gerritsen, G.C., Blanks, M.C.: Characterization of Chinese hamsters by metabolic balance, glucose tolerance and insulin secretion. Diabetologia10, 493–499 (1974)PubMedGoogle Scholar
  23. 23.
    Coleman, D.L., Hummel, K.P.: Studies with the mutation-diabetes in the mouse. Diabetologia3, 238–248 (1967)PubMedGoogle Scholar
  24. 24.
    Nakamura, M., Yamada, K.: Studies on a diabetic KK strain of mouse. Diabetologia3, 212–221 (1967)PubMedGoogle Scholar
  25. 25.
    Allison, R.S.: Carbohydrate tolerance in overweight and obesity. Lancet 1927I, 537–540Google Scholar
  26. 26.
    Joslin, E.P., Dublin, L.T., Marks, H.H.: Studies in diabetes mellitus. IV. Etiology. Amer. J. med. Sci.192, 9–23 (1936)Google Scholar
  27. 27.
    Martin, M.M., Martin, A.L.A.: Obesity, hyperinsulinism and diabetes mellitus in childhood. J. Pediat.82, 192–201 (1973)PubMedGoogle Scholar
  28. 28.
    Stein, S.P., Charles, E.: Emotional factors in juvenile diabetes mellitus: A study of early life experience of adolescent diabetics. Amer. J. Psychiat.128, 700–704 (1971)PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • G. C. Gerritsen
    • 1
  • M. C. Blanks
    • 1
  • R. L. Miller
    • 1
  • W. E. Dulin
    • 1
  1. 1.Diabetes and Atherosclerosis ResearchThe Upjohn CompanyKalamazooUSA

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