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Journal of Cancer Research and Clinical Oncology

, Volume 122, Issue 10, pp 585–589 | Cite as

Frequent loss in chromosome 8p loci in liver cirrhosis accompanying hepatocellular carcinoma

  • Yosuke Kishimoto
  • Goshi Shiota
  • Kouichirou Wada
  • Masayuki Kitano
  • Kentaro Nakamoto
  • Yoshinori Kamisaki
  • Takeaki Suou
  • Tadao Itoh
  • Hironaka Kawasaki
Original Paper Experimental Oncology

Abstract

Most hepatocellular carcinoma (HCC) is preceded by liver cirrhosis, but the genetic changes involved in cirrhosis are not well understood. We therefore studied loss of heterozygosity (LOH) in cirrhotic and neoplastic foci in livers of 14 patients with HCC. The samples, microdissected from paraffin-embedded tissues, were analyzed using a polymerase-chain-reaction-based assay for dinucleotide repeat polymorphisms on 8p. Of the 14 cases, 13 showed constitutional heterozygosity for the microsatellite markers. In 7 (54%) of these 13 informative cases, LOH was detected in the primary HCC and, in these 7 doubly informative (informative and LOH-positive in primary HCC) cases, LOH was found in 16 (70%) of 23 liver cirrhotic foci. The pattern of 8p allelic loss was identical in each doubly informative tumor; however, some of the liver cirrhotic foci harbored an 8p loss identical to that seen in the primary HCC, some harbored a different 8p loss, and some did not harbor any 8p loss. The remaining 6 cases without LOH on 8p in HCC showed no 8p loss in any cirrhotic foci. Presumably HCC could develop from cirrhotic cells harboring 8p loss.

Key words

Hepatocellular carcinoma Liver cirrhosis Loss of heterozygosity Chromosome 8p 

Abbreviations

HCC

hepatocellular carcinoma

LOH

loss of heterozygosity

PCR

polymerase chain reaction

AH

adenomatous hyperplasia

AAH

atypical adenomatous hyperplasia

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Yosuke Kishimoto
    • 1
    • 2
  • Goshi Shiota
    • 2
  • Kouichirou Wada
    • 1
  • Masayuki Kitano
    • 2
  • Kentaro Nakamoto
    • 1
  • Yoshinori Kamisaki
    • 1
  • Takeaki Suou
    • 2
  • Tadao Itoh
    • 1
  • Hironaka Kawasaki
    • 2
  1. 1.Department of Clinical Pharmacology, Faculty of MedicineTottori UniversityYonagoJapan
  2. 2.Second Department of Internal Medicine, Faculty of MedicineTottori UniversityYonagoJapan

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