Journal of Molecular Evolution

, Volume 41, Issue 3, pp 338–344 | Cite as

Structure of the human CRFB4 gene: Comparison with its IFNAR neighbor

  • G. Lutfalla
  • M. G. McInnis
  • S. E. Antonarakis
  • G. Uzé
Articles

Abstract

The cytokine receptor family consists of a growing number of structurally and evolutionarily related transmembrane receptors. CRFB4 and IFNAR are two of the most similar members of this family. They are encoded by two neighboring genes on both human chromosome 21 and murine chromosome 16. The sequence of the human CRFB4 gene was determined from the first exon to the last intron. The nature of the repetitive sequences present in the introns was analyzed and compared with those present in the human IFNAR gene. This analysis leads to considerations of the antiquity of the duplication that gave rise to both genes from a common ancestor. A pseudogene for USF has been identified in the IFNAR gene and a new definition for the repetitive sequence MER37 is proposed. The polymorphism associated with two CA repeats present in the CRFB4 gene is described.

Key words

Repeated sequences Gene evolution Genetic polymorphism Anchored reference locus Interferon receptor 

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References

  1. Batzer MA, Gudi VA, Mena JC, Foltz DW, Herrera RJ, Deininger PL (1991) Amplification dynamics of human-specific (HS) alu family members. Nucleic Acids Res 19:3619–3623Google Scholar
  2. Bazan JF (1990) Structural design and molecular evolution of a cytokine receptor superfamily. Proc Natl Acad Sci USA 87:6934–6938Google Scholar
  3. Economou EP, Bergen AW, Warren AC, Antonarakis SE (1990) The polyadenylate tract ofAlu repetitive elements is polymorphic in the human genome. Proc Natl Acad Sci USA 87:2951–2954Google Scholar
  4. Gregor PD, Sawadogo M, Roeder RG (1990) The adenovirus major late transcription factor USF is a member of the helix-loop-helix group of regulatory proteins and binds to DNA as a dimer. Genes Dev 4:1730–1740Google Scholar
  5. Iris FJM, Bougueleret L, Prieur S, Caterina D, Primas G, Perrot V, Jurka J, Rodriguez-Tome P, Claverie JM, Dausset J, Cohen D (1993) Dense Alu clustering and a potential new member of the NfκB family within a 90 kilobase HLA class III segment. Nature Genet 3:137–144Google Scholar
  6. Jurka J, Milosavljevic A (1991) Reconstruction and analysis of human Alu genes. J Mol Evol 32:105–121Google Scholar
  7. Jurka J, Walichiewicz J, Milosavljevic A (1992) Prototypic sequences for human repetitive DNA. J Mol Evol 35:286–291Google Scholar
  8. Jurka J, Kaplan DJ, Duncan CH, Walichiewicz J, Milosavljevic A, Murali G, Solus JF (1993) Identification and characterization of new human medium reiteration frequency repeats. Nucleic Acids Res 21:1273–1279Google Scholar
  9. Korenberg JR, Rykowski MC (1988) Human genome organization: Alu, Lines, and the molecular structure of metaphase chromosome bands. Cell 53:391–400Google Scholar
  10. Kremer E, Baker E, D'Andrea RJ, Slim R, Phillips H, Moretti PAB, Lopez AF, Petit C, Vadas MA, Sutherland GR, Goodal GJ (1993) Acytokine receptor gene cluster in the X-Y pseudoamosomal region? Blood 82:22–28Google Scholar
  11. Lutfalla G, Gardiner K, Proudhon D, Vielh E, Uzé G (1992) The structure of the human interferon alpha/beta receptor gene. J Biol Chem 267:2802–2809Google Scholar
  12. Lutfalla G, Gardiner K, Uzé G (1993) A new member of the cytokine receptor gene family maps on chromosome 21 at less than 35 kb from IFNAR. Genomics 16:366–373Google Scholar
  13. McInnis MG, Lutfalla G, Slaugenhaupt S, Peterson MB, Uzé G, Chakravard A, Antonarakis SE (1991) Linkage mapping of highly informative DNA polymorphisms within the human interferon alpha receptor gene on chromosome 21. Genomics 11:573–576Google Scholar
  14. Milatovich A, Kitamura T, Miyajima A, Francke U (1993) Gene for the α-subunit of the human interleukin-3 receptor (IL3RA) localized to the X-Y pseudoamosomal region. Am J Hum Genet 53:1146–1153Google Scholar
  15. Nelson DL, Ledbetter SA, Corbo L, Victoria MF, Ramirez-Solis R, Webster TD, Ledbetter DH, Caskey CT (1989) Alu polymerase chain reaction: a method for rapid isolation of human-specific sequences from complex DNA sources. Proc Natl Acad Sci USA 86:6686–6690Google Scholar
  16. Pearson PL, Kidd KK, Willard HF (1987) Report of the committee on human gene mapping by recombinant DNA techniques. Cytogenet Cell Genet 46:390–566Google Scholar
  17. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning, a laboratory manual, second edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NYGoogle Scholar
  18. Shen MR, Batzer MA, Deininger PL (1991) Evolution of the master Alu gene(s). J Mol Evol 33:311–320Google Scholar
  19. Smit AFA (1993) Identification of a new, abundant superfamily of mammalian LTR-transposons. Nucleic Acids Res 21:1863–1872Google Scholar
  20. Stallings RL, Torney DC, Hildebrand CE, Longmire JL, Deaven LL, Jett JH, Doggett NA, Moyzis RK (1990) Physical mapping of human chromosomes by repetitive sequence fingerprinting. Proc Natl Acad Sci USA 87:6218–6222Google Scholar
  21. Tanzi RE, Romano DM, Berger R, Buraczinska MJ, Gaston SM, Kumit DM, Patterson D, Gusella JF, Steward GD (1992) Sequence-tagged sites (STSs) for a set of mapped markers on chromosome 21. Genomics 14:498–502Google Scholar
  22. Thoreau E, Petridou B, Kelly PA, Djiane J, Mornon J-P (1991) Structural symmetry of the extracellular domain of the cytokine/growth hormone/prolactin receptor family and interferon receptors revealed by hydrophobic cluster analysis. FEBS Lett 282:16–31Google Scholar
  23. Uzé G, Lutfalla G, Gresser I (1990) Genetic transfer of a functional human interferon alpha receptor into mouse cells: cloning and expression of its cDNA. Cell 60:225–234Google Scholar
  24. Watkins P, Tanzi RE, Roy J, Stuart J, Stanislovitis P, Gusella JF (1987) A cosmid clone genetic map of chromosome 21 and localisation of the breast cancer estrogen-inducible (BCEI) gene. Cytogenet Cell Genet 46:712Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • G. Lutfalla
    • 1
  • M. G. McInnis
    • 2
  • S. E. Antonarakis
    • 2
  • G. Uzé
    • 1
  1. 1.Institut de Génétique MoléculaireCNRS UMR 9942Montpellier Cedex 1France
  2. 2.Departments of Pediatrics and MedicineThe Johns Hopkins University School of MedicineBaltimoreUSA

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