Radiation and Environmental Biophysics

, Volume 27, Issue 2, pp 123–132 | Cite as

Action of gamma endonuclease on clustered lesions in irradiated DNA

  • E. Kohfeldt
  • H. Bertram
  • U. Hagen
Article
Received:
Accepted:

Summary

Irradiation of DNA in situ i.e. in phage particles or in the cell leads to alterations of single DNA nucleotides as well as to clustered lesions such as double strand breaks or unpaired DNA regions the latter being sensitive to digestion by S 1 nuclease. A contribution will be made to the configuration of such S 1-nuclease-sensitive sites (S 1 sites). DNA from irradiated lambda phage containing S 1 sites was treated with gamma endonuclease fromM. luteus which is known to split the nucleotide strand at the position of oxidized pyrimidine base. It was found that the gamma endonuclease induces double-strand breaks at some of the S 1 sites indicating double base damage within this site. However, half of the S 1 sites are not converted into a double-strand break by the gamma endonuclease, indicating base damage only on one strand within the unpaired region.

Keywords

Nucleotide Pyrimidine Strand Break Phage Particle Lambda Phage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • E. Kohfeldt
    • 1
  • H. Bertram
    • 1
  • U. Hagen
    • 1
  1. 1.Gesellschaft für Strahlen- und Umweltforschung (GSF) MünchenInstitut für StrahlenbiologieNeuherbergGermany

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