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Hyperthermia enhances the cytotoxicity and platinum-DNA adduct formation of lobaplatin and oxaliplatin in cultured SW 1573 cells

  • R. C. Rietbroek
  • P. J. M. van de Vaart
  • J. Haveman
  • F. A. Blommaert
  • A. Geerdink
  • P. J. M. Bakker
  • C. H. N. Veenhof
Original Paper Experimental Oncology

Abstract

The cytotoxicity of cisplatin and cisplatin-DNA adduct formation in vitro and in vivo is clearly enhanced by hyperthermia. We investigated whether cytotoxicity and platinum-DNA adduct formation of two promising new third-generation platinum derivatives, lobaplatin [1,2-diamminomethylcyclobutane platinum(II) lactate] and oxaliplatin [oxalato-1,2-diaminocyclohexane platinum(II)], are also enhanced by hyperthermia. Cisplatin was used for comparison. SW 1573 cells were incubated with cisplatin, lobaplatin or oxaliplatin at different concentrations for 1 h at 37°, 41° and 43°C. The reproductive capacity of cells was determined by cloning experiments. Immunocytochemical detection of platinum-DNA adducts was performed with the rabbit antiserum NKI-A59. At 37°C, cisplatin was the most cytotoxic, followed by oxaliplatin and lobaplatin. Hyperthermia clearly enhanced the cytotoxicity of cisplatin, lobaplatin and oxaliplatin. There was no further increase in cytotoxicity at 43°C compared to 41°C for cisplatin and oxaliplatin. A further increase in cytotoxicity at 43°C was observed for lobaplatin. At 43°C thermal enhancement was higher for lobaplatin than for oxaliplatin, with the reverse pattern at 41°C. For both drugs, thermal enhancement of cytotoxicity was lower than observed for cisplatin. Immunocytochemical detection of platinum-DNA adducts was feasible for all the drugs. Adduct formation was enhanced at 43°C for cisplatin, lobaplatin and oxaliplatin with a relative increase of 410%, 170% and 180%. These results seem to confirm that an increase in platinum-DNA adduct formation is involved in the in vitro thermal enhancement of cytotoxicity. The observed thermal enhancement of cytotoxicity of lobaplatin and oxaliplatin in vitro warrants further in vivo investigations.

Key words

Hyperthermia induced Lobaplatin Oxaliplatin Cisplatin Platinum-DNA adduct formation Cytotoxicity 

Abbreviation

TER

thermal enhancement ratio

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References

  1. Barlogie B, Corry PM, Drewinko B (1980) In vitro thermochemotherapy of human colon cancer cells withcis-dichlorodiammine-platinum(II) and mitomycin C. Cancer Res 40:1165–1168PubMedGoogle Scholar
  2. Benthem J van, Wild CP, Vermeulen E, Engelse L den, Scherer E (1991) Immunocytochemical localization of DNA adducts induced by a single dose ofN-nitroso-N-methylbenzylamine in target and non-target tissues of tumor formation in the rat. Carcinogenesis 12:1831–1837PubMedGoogle Scholar
  3. Blommaert FA, Saris CP (1995) Detection of platinum-DNA adducts by 32P-postlabelling. Nucleic Acids Res 23:126–132Google Scholar
  4. Blommaert FA, Michael C, Terheggen PMAB, Muggia FM, Kortes V, Schornagel JH, Hart AAM, Engelse L den (1993) Drug induced DNA modification in buccal cells of cancer patients receiving carboplatin and cisplatin combination chemotherapy, as determined by an immunocytochemical method: interindividual variation and correlation with disease response. Cancer Res 53:5669–5675PubMedGoogle Scholar
  5. Dahl O, Mella O (1990) Hyperthermia and chemotherapeutic agents. In: Field SB, Hand JW (eds) Introduction to the practical aspects of clinical hyperthermia. Taylor and Francis, London, pp 108–133Google Scholar
  6. Dijk JDP van, Schneider C, Os R van, Blank LECM, Gonzalez Gonzalez D (1990) Results of deep body hyperthermia with large wave guide radiators. In: Bicher HI (ed) Consensus on hyperthermia for the 1990's. Plenum, New York, pp 315–319Google Scholar
  7. Extra JM, Espie M, Calvo F, Ferme C, Mignot L, Marty M (1990) Phase I study of oxaliplatin in patients with advanced cancer. Cancer Chemother Pharmacol 25:299–303PubMedGoogle Scholar
  8. Fichtinger-Schepman AMJ, Baan RA, Luiten-Schulte A, van Dijk M, Lohman PHM (1985) Immunochemical quantitation of adducts in DNA bycis-diammine dichloroplatinum (II) and analysis of adduct related DNA-unwinding. Chem Biol Interact 55:275–288PubMedGoogle Scholar
  9. Fichtinger-Schepman AMJ, Baan RA, Berends F (1989) Influence of the degree of DNA modification on the immunochemical determination of cisplatin-DNA adduct levels. Carcinogenesis 10:2367–2369PubMedGoogle Scholar
  10. Fiebig HH, Berger DP, Mross K, Quiber W, Aulenbacher P, Hilgard P (1992) Lobaplatin (D-19466): preclinical drug profile and results of the phase I study. Ann Oncol 3 [Suppl 1]:338Google Scholar
  11. Fisher GA, Hahn GM (1982) Enhancement of cisplatinum (II) diamminedichloride by hyperthermia. Natl Cancer Inst Monogr 61:255–257Google Scholar
  12. Gerad H, Egorin MJ, Whitacre M, Van Echo DA, Aisner J (1983) Renal failure and platinum pharmacokinetics in three patients treated with cisdiamminedichloro-platinum (II) and whole body hyperthermia. Cancer Chem Pharmacol 11:162–166Google Scholar
  13. Gietema JA, Veldhuis GJ, Guchelaar HJ, Willemse PH, Uges DR, Cats A, Boonstra H, van der Graaf WT, Sleijfer DT, de Vries EG, Mulder NH (1995) Phase II and pharmacokinetic study of lobaplatin in patients with relapsed ovarian cancer. Br J Cancer 71:1302–1307PubMedGoogle Scholar
  14. Haas GP, Klugo RC, Hetzel FW, Barton EE, Cerny JC (1984) The synergistic effect of hyperthermia and chemotherapy on murine transitional cell carcinoma. J Urol 132:828–833PubMedGoogle Scholar
  15. Hahn GM, Li GC (1982) Interaction of hyperthermia and drugs: treatments and probes. Natl Cancer Inst Monogr 61:317PubMedGoogle Scholar
  16. Issels RD, Prenninger SW, Nagele A, Boehm E, Sauer H, Jauch KW, Denecke H, Berger H (1990) Ifosfamide plus etoposide combined with regional hyperthermia in patients with locally advanced sarcomas: a phase II study. J Clin Oncol 8:1818–1829PubMedGoogle Scholar
  17. Kelland LR (1993) New platinum antitumour complexes. Crit Rev Oncol Hematol 15:191–219PubMedGoogle Scholar
  18. Leng M (1990) Distortions induced in double-stranded oligonucleotides by the binding ofcis- ortrans-diammine dichloroplatinum(II) to the d(GTG) sequence. Nucleic Acids Res 18:4395PubMedGoogle Scholar
  19. Levi F, Perpoint B, Garufi C, Focan C, Chollet P, Depres-Brummer P, Zidani R, Brienza S, Itzhaki M, Iacobelli S, Kunstlinger F, Gastiaburu J, Misset JL (1993) Oxaliplatin activity against metastatic colorectal cancer. A phase II study of 5 days continuous venous infusion at circadian rhythm modulated rate. Eur J Cancer 29A:1280–1284Google Scholar
  20. Levi FA, Zidani R, Vannetzel JM, Perpoint B, Focan C, Faggiuolo R, Chollet P, Garufi C, Itzhaki M, Dogliotti L, Iacobelli S, Adam R, Kunstlinger F, Gastiaburu J, Bismut H, Jasmin C, Misset JL (1994) Chrono-modulated versus fixed-infusion-rate delivery of ambulatory chemotherapy with oxaliplatin, fluorouracil, and folinic acid (leucovorin) in patients with colorectal cancer metastases: a randomized multi-institutional trial. J Nat Cancer Inst 86:1608–1617PubMedGoogle Scholar
  21. Los G, Sminia P, Wondergem J, Mutsaers PHA, Haveman J, Bokkel Huinink D ten, Smals OAG, Gonzalez Gonzalez D, McVie JG (1991) Optimisation of intraperitoneal cisplatin therapy with regional hyperthermia in rats. Eur J Cancer 27:472–477PubMedGoogle Scholar
  22. Los G, Vugt MJH van, Engelse L den, Pinedo HM (1993) Effects of temperature on the interaction of cisplatin and carboplatin with cellular DNA. Biochem Pharmacol 46:1229–1237PubMedGoogle Scholar
  23. Los G, Vugt MJH van, Pinedo HM (1994) Response of peritoneal solid tumours after intraperitoneal chemohyperthermia treatment with cisplatin or carboplatin. Br J Cancer 69:235–241PubMedGoogle Scholar
  24. Mathe G, Kidani Y, Segiguchi M, Eriguchi M, Fredi G, Peytavin G, Misset JL, Brienza, S, De Vassals F, Chenu E (1989) L-OHP, a third generation platinum complex. An experimental and clinical appraisal and preliminary comparison with cisplatin and carboplatin. Biomed Pharmacother 43:237–250PubMedGoogle Scholar
  25. Mella O, Eriksen R, Dahl O, Laenum OD (1987) Acute systemic toxicity of combinedcis-diamminedichloroplatinum and hyperthermia in the rat. Eur J Cancer Clin Oncol 23:365–373PubMedGoogle Scholar
  26. Murthy MS, Rao LN, Khandehar JD, Scanlon EF (1987) Enhancement of the therapeutic efficacy of cisplatin by combination with diethyl dithiocarbonate and hyperthermia in a mouse model. Cancer Res 47:774–779PubMedGoogle Scholar
  27. Overgaard J, Gonzalez Gonzalez D, Hulshof MCMM, Arcangeli G, Dahl O, Mella O, Brentzen SM (1995) Randomised trial of hyperthermia as adjuvant to radiotherapy for recurrent or metastatic malignant melanoma. Lancet 345:540–543PubMedGoogle Scholar
  28. Rice JA, Crothers DM, Pinto AL, Lippard SJ (1989) The major adduct of the antitumor drugcis-diamminedichloroplatinum (II) with DNA bends the duplex by about 40 degrees towards the major groove. Proc Natl Acad Sci USA 85:4158–4161Google Scholar
  29. Rietbroek R, Schilthuis M, Postma A, Zum Vörde Sive Vörding P, Gonzalez Gonzalez D, Bakker A, Bakker P, Veenhof C (1995) Weekly locoregional hyperthermia and cisplatin in patients with a local recurrence of a cervical carcinoma. Proc ASCO 14:287Google Scholar
  30. Rietbroek RC, Bakker PJM, Schilthuis MS, Postma AJ, Zum Vörde Sive Vörding PJ, Gonzalez Gonzalez D, Kurth KH, Bakker AJ, Veenhof CHN (1996) Feasibility, toxicity and preliminary results of weekly locoregional hyperthermia and cisplatin in patients with previously irradiated recurrent cervical cancer or locally advanced bladder cancer. Int J Radiat Oncol Biol Phys 34:887–893PubMedGoogle Scholar
  31. Robins HI, Cohen JD, Schmitt CL, Tutsch KD, Feierabend C, Arzoomanian RZ, Alberti D, D'Oleire F, Longo W, Heiss C, Rushing D, Spriggs D (1993) Phase I clinical trial of carboplatin and 41.8°C whole-body hyperthermia in cancer patients. J Clin Oncol 11:1787–1794PubMedGoogle Scholar
  32. Stege GJJ, Kampinga HH, Konings AWT (1995) Heat-induced intranuclear protein aggregation and thermal radiosensitization. Int J Radiat Biol 67:203–209PubMedGoogle Scholar
  33. Stewart DJ, Benjamin RS, Luna M, Feun L, Caprioli R, Seiffert W, Loo TL (1982) Human tissue distribution of platinum after cis-diamminedichloroplatinum. Cancer Chemother Pharmacol 10:51–54PubMedGoogle Scholar
  34. Tashiro T, Kawada Y, Sahurai Y, Kidani Y (1989) Antitumour activity of a new platinum complex, oxalato (trans-1-1,2-diamminocyclohexane)platinum II: new experimental data. Biomed Pharmacother 43:251–260PubMedGoogle Scholar
  35. Terheggen PMAB, Dijkman R, Begg AC, Dubbelman R, Floot BGJ, Hart AAM, Engelse L den (1988) Monitoring of interaction products ofcis-diamminedichloroplatinum (II) andcis-diammine(1,1-cyclo-butanedicarboxylato) platinum (II) with DNA in cells from platinum treated cancer patients. Cancer Res 48:5597–5603PubMedGoogle Scholar
  36. Terheggen PMAB, Emondt JY, Floot BGJ, Dijkman R, Schrier PI, Engelse L den, Begg AC (1990) Correlation between cell killing bycis-diamminedichloroplatinum(II) in six mammalian cell lines and binding of acis-diamminedichloroplatinum (II)-DNA antiserum. Cancer Res 50:3556–3561PubMedGoogle Scholar
  37. Terheggen PMAB, Floot BGJ, Lempers ELM, Van Tellingen O, Begg AC, Engelse L den (1991) Antibodies against cisplatin-modified DNA and cisplatin-modified (di)nucleotides. Cancer Chemother Pharmacol 28:185–191PubMedGoogle Scholar
  38. Urano M, Kahn J, Majima H, Gerwech LE (1990) The cytotoxic effect of cisdiammine dichloroplatinum (II) on cultured Chinese hamster ovary cells at elevated temperatures: arrhenius plot analysis. Int J Hyperthermia 6:581–590PubMedGoogle Scholar
  39. Voegeli R, Schumacher W, Engel J, Respondek J, Hilgard P (1990) D-19466 a new cyclobutane-platinum complex with antitumour activity. J Cancer Res Clin Oncol 116:419–422Google Scholar
  40. Weiss R, Michaele C (1993) New cisplatin analogues in development. A review. Drugs 46:360–377PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • R. C. Rietbroek
    • 1
  • P. J. M. van de Vaart
    • 3
  • J. Haveman
    • 2
  • F. A. Blommaert
    • 3
  • A. Geerdink
    • 2
  • P. J. M. Bakker
    • 1
  • C. H. N. Veenhof
    • 1
  1. 1.Department of Medical Oncology, F4-224Academic Medical CentreAmsterdamThe Netherlands
  2. 2.Department of Radiotherapy, University of AmsterdamAcademic Medical CentreAmsterdamThe Netherlands
  3. 3.The Netherlands Cancer InstituteAmsterdamThe Netherlands

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