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Journal of Gastroenterology

, Volume 31, Issue 1, pp 12–17 | Cite as

Expression of CD10/neutral endopeptidase in normal and malignant tissues of the human stomach and colon

  • Yukihiko Sato
  • Fumio Itoh
  • Yuji Hinoda
  • Yasuo Ohe
  • Naoaki Nakagawa
  • Ryuzo Ueda
  • Akira Yachi
  • Kohzoh Imai
Article

Abstract

The expression of common acute lymphoblastic leukemia antigen (CALLA; CD10), which is identical to neutral endopeptidase (NEP, EC3.424.11), was examined in the malignant and adjacent noninvaded tissues of the human stomach and colon (n = 27). All of 27 normal and 18 well or moderately differentiated adenocarcioma tissue specimens were positive for monoclonal antibody (mAb) NL-1 against CD10/NEP, whereas the expression level was clearly decreased in all of 9 specimens of poorly differentiated adenocarcinoma. In addition, all of 7 gastric or colorectal carcinoma cell lines tested showed decreased expression of CD10/NEP. Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis of the crude antigen J5 from the normal colon tissue lysate by mAb J5 detected a single band of approximately 100 kDa that was consistent with that of NALM-6 cells used as a positive control. These findings suggest that CD10/NEP is expressed in normal epithelial cells of the human stomach and colon, whereas the expression level is decreased in the poorly differentiated type of adenocarcinoma.

Key words

CD10 neutral endopeptidase stomach colon carcinoma 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Yukihiko Sato
    • 1
  • Fumio Itoh
    • 1
  • Yuji Hinoda
    • 1
  • Yasuo Ohe
    • 1
  • Naoaki Nakagawa
    • 1
  • Ryuzo Ueda
    • 2
  • Akira Yachi
    • 3
  • Kohzoh Imai
    • 1
  1. 1.First Department of Internal MedicineSapporo Medical UniversitySapporoJapan
  2. 2.Laboratory of ChemotherapyAichi Cancer Center Research InsitutegoyaJapan
  3. 3.Sapporo Medical UniversitySapporoJapan

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