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Effects of okadaic acid on cell growth, anchorage-independent growth, and co-cultures of normal (KMS-6), immortalized (KMST-6), and neoplastically transformed (KMST-6T and KMST-6/RAS) human fibroblasts

  • Israt Jahan
  • Mikio Iijima
  • Tadashi Kondo
  • Masayoshi Namba
Original Paper Experimental Oncology

Abstract

The effects of okadaic acid (OA) on normal human (KMS-6), its immortalized (KMST-6) and neoplastically transformed (KMST-6T and KMST-6/RAS) cells were investigated as a model of two-stage carcinogenesis. The presence of OA inhibited cell growth of the normal and immortalized cells but not that of the neoplastic KMST-6T cells. In contrast, cell growth of the other neoplastic KMST-6/RAS cells transformed with the Ha-ras oncogene was inhibited by OA. OA enhanced colony formation of KMST-6T cells in soft agar, but it suppressed that of KMST-6/RAS cells. Co-cultures of KMST-6T cells with normal KMS-6 cells showed an increase in focus formation of KMST-6T cells in the presence of OA, whereas focus formation of KMST-6/RAS cells decreased. These results indicate that OA has growth-promoting effects on certain types of transformed human cells.

Key words

Tumor promoter Okadaic acid Human transformed cells Anchorage-independent growth Cell communication 

Abbreviation

OA

okadaic acid

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Israt Jahan
    • 1
  • Mikio Iijima
    • 1
  • Tadashi Kondo
    • 1
  • Masayoshi Namba
    • 1
  1. 1.Department of Cell Biology, Institute of Cellular and Molecular BiologyOkayama University Medical SchoolOkayamaJapan

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