Advertisement

Matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 expression in paediatric tumour cells. Effects of tumour cell proliferation modulators on gelatinolytic activity

  • Rocío Garcia de Veas
  • Lothar Schweigerer
  • Miguel Angel Medina
Original Paper Experimental Oncology

Abstract

We have examined the expression of 72-kDa gelatinase/type IV collagenase or matrix metalloproteinase-2 (MMP-2) and ist inhibitor, tissue inhibitor of metalloproteinase-2 (TIMP-2), in various cell lines derived from paediatric tumours. In a neuroblastoma model system of tumour progression, the expression level of MMP-2 mRNA was higher in the more malignant cell line. Surprisingly, MMP-2 was not expressed in the highly malignant rhabdomyosarcoma A-204 cell line. TIMP-2 showed higher expression levels in the 007 and U-2OS tumour cell lines than in the more malignant ones, WAC2 and A-204 cells. We have also determined the effect of some tumour cell proliferation modulators on gelatinolytic activity. While basic fibroblast growth factor and retinoic acid produced no apparent change in gelatinolytic activity, genistein induced in partial inhibition of gelatinolytic activity.

Key Words

72-kDa gelatinase Type IV collagenase MMP-2 TIMP-2 Neuroblastoma Osteosarcoma Rhabdomyosarcoma Genistein 

Abbreviations

MMP

matrix metalloproteinase

TIMP

tissue inhibitor of metalloproteinases

bFGF

basic fibroblast growth factor

GAPDH

glyceraldehyde-3-phosphate dehydrogenase

ECM

extracellular matrix

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Akiyama T, Ishida J, Nakagawa S, Ogawara H, Watanabe S, Itoh N, Shibuya M, Fuami Y (1987) Genistein, a specific inhibitor of tyrosine-specific protein kinases. J Biol Chem 262: 5592–5595PubMedGoogle Scholar
  2. Basset P, Bellocq JP, Wolf C, Stoll I, Hutin P, Limacher JM, Podhajcer OL, Chenard MP, Río MC, Chambon P (1990) A novel metalloproteinase gene specifically expressed in stromal cells of breast carcinomas. Nature 348: 699–704PubMedGoogle Scholar
  3. Briozzo P, Morisset M, Capony F, Rougeot G, Rochefort H (1988) In vitro degradation of extracellular matrix withM r 52,000 cathepsin D secreted by breast cancer cells. Cancer Res 48: 3688–3692PubMedGoogle Scholar
  4. Burgess WH, Maciag T (1989) The heparin-binding (fibroblast) growth factor family of proteins. Annu Rev Biochem 58: 575–606PubMedGoogle Scholar
  5. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–159PubMedGoogle Scholar
  6. Collier IE, Wilhelm SM, Eisen AZ, Marmer BL, Grant GA, Seltzer JL, Kronberger A, He C, Bauer EA, Goldberg C (1988) H-ras oncogene transformed human bronchial epithelial cells (TBE-1) secrete a single metalloprotease capable of degrading basement membrane collagen. J Biol Chem 263: 6579–6587PubMedGoogle Scholar
  7. Danø K, Andreasen PA, Grondahl-Hansen J, Kristensen P, Nielsen LS, Skriver L (1985) Plasminogen activators, tissue degradation and cancer. Adv Cancer Res 45: 139–266Google Scholar
  8. Favennec L, Cals MJ (1988) The biological effect of retinoids on cell differentiation and proliferation. J Clin Chem Clin Biochem 26: 479–489PubMedGoogle Scholar
  9. Folkman J, Shing Y (1992) Angiogenesis. J Biol Chem 267: 10931–10934PubMedGoogle Scholar
  10. Fotsis T, Pepper M, Adlercreutz H, Fleischmann G, Hase T, Montesano R, Schweigerer L (1993) Genistein, a novel dietary-derived angiogensis inhibitor. Proc Natl Acad Sci USA 90: 2690–2694PubMedGoogle Scholar
  11. Gottesman M (1990) The role of proteases in cancer. Semin Cancer Biol 1: 97–160Google Scholar
  12. Kao RT, Wong M, Stern R (1982) Elastin degradation by proteinases from cultured human breast cancer cells. Biochem Biophys Res Commun 105: 383–389PubMedGoogle Scholar
  13. Liotta LA, Tryggvason K, Garbisa S, Hart IR, Foltz CM, Shaflie S (1980) Metastatic potential correlates with enzymatic degradation of basement membrane collagen. Nature 284: 67–69PubMedGoogle Scholar
  14. Liotta LA, Rao CN, Barsky SH (1983) Tumor invasion and the extracellular matrix. Lab Invest 49: 636–649PubMedGoogle Scholar
  15. Liotta LA, Steeg PS, Steller-Stevenson WG (1991) Cancer metastasis and angiogenesis: an imbalance of positive and negative regulation. Cell 64: 327–336PubMedGoogle Scholar
  16. Medina MA, Del Castillo-Olivares A, Schweigerer L (1992) Plasma membrane redox activity correlates with N-myc expression in neuroblastoma cells. FEBS Lett 311: 99–101PubMedGoogle Scholar
  17. Miyazaki K, Hattori Y, Umenishi F, Yasumitsu H, Umeda M (1990) Purification and characterization of extracellular matrix-degrading metalloproteinase, matrin (pump-1), secreted from human rectal carcinoma cell line. Cancer Res 50: 7758–7764PubMedGoogle Scholar
  18. Murphy G, Cawston T, Reynolds J (1981) An inhibitor of collagenase from human amniotic fluid. Purification, characterization and action on metalloproteinases. Biochem J 195: 167–170PubMedGoogle Scholar
  19. Nakajima M, Welch D, Belloni PN, Nicolson GL (1987) Degradation of basement membrane type IV collagen and lung subendothelial matrix by rat mammary adenocarcinoma cell clones of differing metastatic potentials. Cancer Res 47: 4869–4876PubMedGoogle Scholar
  20. Okada Y, Gonoki Y, Naka K, Tomita K, Nakanishi I, Iwata K, Yamashita K, Hayakawa T (1992) Matrix metalloproteinase 9 (92 kDa gelatinase/type IV collagenase) from HT 1080 human fibrosarcoma cells. J Biol Chem 267:21712–21719PubMedGoogle Scholar
  21. Poole AR, Tiltman KJ, Recklies AD, Stocker TAM (1990) Differences in secretion of the proteinase cathepsine B at the edge of human breast carcinomas and fibrosarcomas. Nature 273: 545–549Google Scholar
  22. Salo T, Liotta LA, Tryggvason K (1983) Purification and characterization of a murine basement membrane collagen-degrading enzyme secreted by metastatic tumor cells. J Biol Chem 258: 3058–3063PubMedGoogle Scholar
  23. Samuel SK, Hurta RAR, Kondaiah P, Khalil N, Turley EA, Wright JA, Greenberg AH (1992) Autocrine induction of tumor protease production and invasion by a metallothioniein-regulated TGF-β1 (Ser223,225). EMBO J 11: 1599–1605PubMedGoogle Scholar
  24. Sappino AP, Busso N, Belin D, Vassali JD (1987) Increase of urokinase type plasminogen activator gene expression in human lung and breast carcinoma. Cancer Res 47: 4043–4046PubMedGoogle Scholar
  25. Schwab M (1990) Amplification of the gene MYCN in human neuroblastoma. Crit Rev Oncogenesis 2: 35–51PubMedGoogle Scholar
  26. Schweigerer L, Neufeld G, Gospodarowicz D (1987) Basic fibroblast growth factor as a growth inhibitor for cultured human tumor cells. J Clin Invest 80: 1516–1520PubMedGoogle Scholar
  27. Schweigerer L, Breit S, Wenzel A, Tsunamoto K, Ludwig R, Schwab M (1990) Augmented MYCN expression advances the malignant phenotype of human neuroblastoma cells: evidence for induction of autocrine growth factor activity. Cancer Res 50: 4411–4416PubMedGoogle Scholar
  28. Schweigerer L, Christeleit K, Fleischmann G, Adlercreutz H, Wähälä K, Hase T, Schwab M, Ludwig R, Fotsis T (1992) Identification in human urine of a natural growth inhibitor for cells derived from solid pediatric tumors. Eur J Clin Invest 22: 260–264PubMedGoogle Scholar
  29. Stetler-Stevenson WG, Krutsch HC, Liotta LA (1989) Tissue inhibitor of metalloproteinase (TIMP-2). J Biol Chem 264: 17374–17378PubMedGoogle Scholar
  30. Troen BR, Ascherman D, Atlas D, Gottesman MM (1988) Cloning and expression of the gene for the major excreted protein of transformed mouse fibroblasts. A secreted lysosomal protease regulated by transformation. J Biol Chem 263: 254–261PubMedGoogle Scholar
  31. Turpeeniemi-Hujanen T, Thorgeirsson UP, Hartl IR, Grant S, Liotta LA (1985) Expresiion of collagenase IV (basement membrane collagenase) activity in murine tumor cell hybrids that differ in metastatic potential. J Natl Cancer Inst 75: 99–108PubMedGoogle Scholar
  32. Urdiales JL, Matés JM, Núñez de Castro I, Sánchez-Jiménez FM (1992) Chlorpheniramine inhibits the ornithine decarboxilase induction of Ehrlich carcinoma growing in vivo. FEBS Lett 305: 260–264PubMedGoogle Scholar
  33. Welgus HG, Jeffrey JJ, Eisen AZ (1981) The collagen substrate specificity of human skin fibroblast collagenase. J Biol Chem 256: 9511–9515PubMedGoogle Scholar
  34. Yang TT, Hawkes SP (1992) Role of the 21 kDa protein TIMP-3 in oncogenic transformation of cultured chicken embryo fibroblasts. Proc Natl Acad Sci USA 89: 10676–10680PubMedGoogle Scholar
  35. Zheng ZS, Polakowska R, Johnson A, Goldsmith LA (1992) Transcriptional control of epidermal growth factor receptor by retinoic acid. Cell Growth Differ 3: 225–232PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Rocío Garcia de Veas
    • 1
  • Lothar Schweigerer
    • 2
  • Miguel Angel Medina
    • 1
    • 2
  1. 1.Laboratorio de Bioquímica y Biología Molecular, Facultad de CienciasUniversidad de MálagaMálagaSpain
  2. 2.Division of Hematology/OncologyChildren's University Hospital, INF 150HeidelbergGermany

Personalised recommendations