Matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 expression in paediatric tumour cells. Effects of tumour cell proliferation modulators on gelatinolytic activity

  • Rocío Garcia de Veas
  • Lothar Schweigerer
  • Miguel Angel Medina
Original Paper Experimental Oncology


We have examined the expression of 72-kDa gelatinase/type IV collagenase or matrix metalloproteinase-2 (MMP-2) and ist inhibitor, tissue inhibitor of metalloproteinase-2 (TIMP-2), in various cell lines derived from paediatric tumours. In a neuroblastoma model system of tumour progression, the expression level of MMP-2 mRNA was higher in the more malignant cell line. Surprisingly, MMP-2 was not expressed in the highly malignant rhabdomyosarcoma A-204 cell line. TIMP-2 showed higher expression levels in the 007 and U-2OS tumour cell lines than in the more malignant ones, WAC2 and A-204 cells. We have also determined the effect of some tumour cell proliferation modulators on gelatinolytic activity. While basic fibroblast growth factor and retinoic acid produced no apparent change in gelatinolytic activity, genistein induced in partial inhibition of gelatinolytic activity.

Key Words

72-kDa gelatinase Type IV collagenase MMP-2 TIMP-2 Neuroblastoma Osteosarcoma Rhabdomyosarcoma Genistein 



matrix metalloproteinase


tissue inhibitor of metalloproteinases


basic fibroblast growth factor


glyceraldehyde-3-phosphate dehydrogenase


extracellular matrix


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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Rocío Garcia de Veas
    • 1
  • Lothar Schweigerer
    • 2
  • Miguel Angel Medina
    • 1
    • 2
  1. 1.Laboratorio de Bioquímica y Biología Molecular, Facultad de CienciasUniversidad de MálagaMálagaSpain
  2. 2.Division of Hematology/OncologyChildren's University Hospital, INF 150HeidelbergGermany

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