Variability of vitamin E content in pumpkin seeds (Cucurbita pepo L)

  • M. Murkovic
  • A. Hillebrand
  • J. Winkler
  • W. Pfannhauser
Original Paper


Pumpkin (Cucurbita pepo L.) seed oil is a common salad oil which is produced in the southern parts of Austria, Slovenia and Hungary. It is dark green and has a high content of free fatty acids. Due to its colour, the oil cannot be used for cooking. The content of vitamin E, especiallyγ-tocopherol, is very high. The oil content of the pumpkin seed is about 50%. The seed itself can be eaten. Therefore a pumpkin variety with high vitamin E content is desirable. The aim of this work was to find a variety ofCucurbita pepo which has a high oil yield and a high vitamin E content. A total of 100 breeding lines were tested for their tocopherol content. The tocopherols and tocotrienols are extracted with hexane and analysed by NP-HPLC/FLD with hexane/dioxan (96/4) as eluent, with fluorescence detection at 292/335 nm. The/gg-tocopherol content, which is about 5–10 times as much as that of α-tocopherol varies over a broad range (41–620 mg/kg dry pumpkin seeds).β- andδ-tocopherol are found at low levels.

Key words

Pumpkin seed oil Vitamin E Tocopherols Tocotrienols Cucurbita pepo 


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  1. 1.
    Schuster W (1977) Adv Agron Crop Sci 4: 5–53Google Scholar
  2. 2.
    Abe K, Matsumoto A (1993) Quantitative determination of tocopherols. In: Mino M, Nakamura H, Diplock AT, Kayden HJ (eds) Vitamin E-Its usefulness in health and in curing diseases. Japan Scientific Societies Press, Tokyo and Karger, BaselGoogle Scholar
  3. 3.
    Nitsch-Fitz R, Egger E, Wutzel H, Maruna H (1979) Acta Med Empirica 12: 1009–1013Google Scholar
  4. 4.
    Dawson RMC, Elliott DC, Elliott WH, Jones KM (1986) Data for biochemical research, 3rd edn. Calendron Press, OxfordGoogle Scholar
  5. 5.
    Tran K, Chan AC (1992) Lipids 27: 38–41PubMedGoogle Scholar
  6. 6.
    Ames SR (1972) Tocopherols: occurrence in foods. In: Sebrell WH, Harris RS (eds) The vitamins, 2nd edn, vol V. Academic Press, New YorkGoogle Scholar
  7. 7.
    Bourgeois C (1992) Determination of vitamin E: tocopherols and tocotrienols. Elsevier, LondonGoogle Scholar
  8. 8.
    Ball GFM (1988) Fat-soluble vitamin assays in food analysis. Elsevier, LondonGoogle Scholar
  9. 9.
    Balz MK, Schulte E, Thier HP (1993) Fat Sci Technol 95: 215–220Google Scholar
  10. 10.
    Deutsche Gesellschaft für Ernährung (1991) Empfehlungen für die Nährstoffzufuhr, 5th edn. Umschau, FrankfurtGoogle Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • M. Murkovic
    • 1
  • A. Hillebrand
    • 1
  • J. Winkler
    • 2
  • W. Pfannhauser
    • 1
    • 2
  1. 1.Department of Bio- and Food ChemistryGraz University of TechnologyGrazAustria
  2. 2.SZGGleisdorfAustria

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