Journal of Assisted Reproduction and Genetics

, Volume 9, Issue 1, pp 41–44 | Cite as

Culture of human spare preembryos: Association between blastocyst formation and pregnancy

  • Anita Sjögren
  • Peter Sjöblom
  • Lars Hamberger
Original Manuscripts
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Accepted:

Abstract

The rate of blastocyst development was studied in cultures of human fertilized eggs. A total of 195 spare embryos from 86 cycles in 73 in vitro fertilization (IVF) patients resulted in a total of 77 expanded blastocysts, corresponding to an overall blastocyst development of 39%. In the group of patients where pregnancy occurred, at least one of the spare embryos developed into the blastocyst stage in 100% of the patients, while the corresponding figure for nonpregnant patients was 53%. Superficially these results seem to indicate that blastocyst development could be of decisive importance for the success rate in IVF. A detailed analysis revealed, however, that this is not the case as long as in vitro culture before replacement is limited to 2–3 days.

Key words

blastocyst embryo culture in vitro fertilization 
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References

  1. 1.
    Sjöblom P, Wikland M: A follow-up study of sperm preparation for IVF by swim-up in a solution of hyaluronate. Hum Reprod 1991;6:722–726Google Scholar
  2. 2.
    Yovich JL: Embryo quality and pregnancy rates in vitro fertilization. Lancet 1985;1:283Google Scholar
  3. 3.
    Cummins JM, Breen TM, Harrison KL, Shaw JM, Wilson LM, Henessey JF: A formula for scoring human embryo growth rates in in vitro fertilization: Its value in predicting pregnancy and in comparison with visual estimates of embryo quality. J Vitro Fert Embryo Transfer 1986;3:284–295Google Scholar
  4. 4.
    Bolton VN, Hawes SM, Taylor CM, Parsons JH: Development of spare human preimplantation embryos in vitro: An analysis of the correlations among gross morphology, cleavage rates, and development to the blastocyst. J Vitro Fert Embryo Transfer 1989;6:30–35Google Scholar
  5. 5.
    Bordson BL, Dickey RP, Dunaway HE, Taylor SN, Curole DN: Correlation of pregnancy following in vitro fertilization (IVF) and embryo transfer (ET) with normal blastulation of sibling embryos remaining in culture. Fifth World Congress on in Vitro Fertilization and Embryo Transfer, Norfolk, VA, USA, 1987, Program supplement, p 74 (abstr)Google Scholar
  6. 6.
    Claman P, Armant DR, Seibel MM, Wang TA, Oskowitz SP, Taymor ML: The impact of embryo quality and quantity on implantation and the establishment of viable pregnancy. J Vitro Fert Embryo Transfer 1987;4:218–222Google Scholar
  7. 7.
    Hill GA, Freeman M, Bastias MC, Rogers BJ, Herbert III CM, Osteen KG, Wentz AC: The influence of oocyte maturity and embryo quality on pregnancy rate in a program for in vitro fertilization-embryo transfer. Fertil Steril 1989;52:801–806Google Scholar
  8. 8.
    Abramovici H, Dirnfeld M, Weisman Z, Sorokin Y, Lissak A, Rofe A, Sheinfeld M: Pregnancies following the interval double-transfer technique in an in vitro fertilization-embryo transfer program. J Vitro Fert Embryo Transfer 1988;5:175–176Google Scholar
  9. 9.
    Caspi E, Ron-El R, Golan A, Herman A, Nachum H: Early, late and sequential embryo transfer in an in vitro fertilization program: A preliminary report. Fertil Steril 1989;52:146–148Google Scholar
  10. 10.
    Fehilly CB, Cohen J, Simons RF, Fishel SB, Edwards RG: Cryopreservation of cleaving embryos and expanded blastocysts in the human: A comparative study. Fertil Steril 1985;44:638–644Google Scholar
  11. 11.
    Cohen J, Simons RF, Edwards RG, Fehilly CB, Fishel SB: Pregnancies following the frozen storage of expanding human blastocysts. J Vitro Fertil Embryo Transfer 1985;2:59–64Google Scholar
  12. 12.
    Hartshorne GM, Elder K, Crow J, Dyson H, Edwards RG: The influence of in-vitro development upon post-thaw survival and implantation of cryopreserved human blastocysts. Hum Reprod 1991;6:136–141Google Scholar
  13. 13.
    Ranoux C, Aubriot FX, Dubuisson JB, Cardone V, Foulot H, Poirot C, Chevalier O: A new in vitro fertilization technique: intravaginal culture. Fertil Steril 1988;49:654–657Google Scholar
  14. 14.
    Asch RH, Balmaceda JP, Cittadini E, Figueroa Casas P, Gomel V, Hohl MK, Johnston I, Leeton J, Rodrigues Escudero FJ, Noss K, Wong PC: Gamete Intrafallopian Transfer. International cooperative study of the first 800 cases.In In Vitro Fertilization and Other Assisted Reproduction, Jones HW Jr, Schrader C (eds). Ann NY Acad Sci 1988;541:722–727Google Scholar
  15. 15.
    Cohen J, Lampe LG, Quigley MM, Saunders DM, Hagglund L: World Congress in Vitro Fertilization and Alternate Assisted Reproduction, 1989Google Scholar
  16. 16.
    Medical Research International and Society of Assisted Reproductive Technology, The American Fertility Society. In vitro fertilization/embryo transfer (IVF/ET) in the United States: 1989 results from the National IVF-ET Registry. Fertil Steril 1991;55:14–23Google Scholar

Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • Anita Sjögren
    • 1
  • Peter Sjöblom
    • 1
  • Lars Hamberger
    • 1
  1. 1.Department of Obstetrics and GynaecologyUniversity of Göteborg, Sahlgrenska University HospitalGöteborgSweden

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