Effects of superoxide dismutase on mouse in vitro fertilization and embryo culture system

  • Takafumi Nonogaki
  • Yoichi Noda
  • Katsuhiko Narimoto
  • Yoh Umaoka
  • Takahide Mori
Animal Investigations

Purpose

We recently found that, for mouse embryos fertilized in vivo, the two-cell block could be attenuated by adding superoxide dismutase (SOD), a scavenger of superoxide radicals, to the culture medium. In this study, we evaluated the effects of SOD on the process of fertilization and on the further development of the embryos fertilized in vitro.

Methods

We performed incubation of mouse epididymal spermatozoa, in vitro fertilization, and further cultivation in Biggers—Whitten—Whittingham's medium supplemented with various concentrations of Cu · Zn—SOD.

Results

High concentrations (2000 µg/ml or more) of SOD prevented loss of motility in mouse sperm over time. The addition of SOD (less than 2000 µg/ml) to the basic medium showed no significant difference in the fertilization rate. Also, no significant difference was observed in the rate of polyspermy or parthenogenesis between the basic and the SOD-supplemented media. However, 18% of the two-cell-stage embryos developed to the expanded blastocyst stage in the 500 µg/ml SOD-supplemented medium, while no blastocysts were found in the basic medium. Furthermore, the addition of SOD 7 hr after insemination increased the expanded blastocyst rate (28%).

Conclusions

These results indicate that the addition of SOD exerts a protecting effect from oxidative stress both on sperm viability and on the development of embryos fertilized in vitro as well as in vivo, while its addition showed no effect on the process of fertilization.

Key words

superoxide dismutase oxidative stress sperm motility mouse in vitro fertilization two-cell block 

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Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • Takafumi Nonogaki
    • 1
  • Yoichi Noda
    • 1
  • Katsuhiko Narimoto
    • 1
  • Yoh Umaoka
    • 1
  • Takahide Mori
    • 1
  1. 1.Department of Gynecology and Obstetrics, Faculty of MedicineKyoto UniversityKyotoJapan

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