Experimental & Applied Acarology

, Volume 11, Issue 4, pp 307–316 | Cite as

Identification of xanthine and hypoxanthine as components of assembly pheromone in excreta of argasid ticks

  • František Dusbábek
  • Petr Šimek
  • Alexandr Jegorov
  • Jan Tříska
Article

Abstract

In addition to guanine, xanthine and hypoxanthine were identified in white spherules in excreta of five species ofArgas andOrnithodoros ticks by a reverse-phase high-performance liquid chromatography (HPLC) and a gas chromatographic method with mass spectrometric detection, (GC/MS). The mutual relationships of these purines in excreta ofArgas (Persicargas) persicus were found to be less than 1.5% for hypoxanthine, less than 9.0% for xanthine and 89.8–98.6%, for guanine. In excreta of other species, the relationships of purines were similar, with the exception ofArgas (A.) reflexus andA. (A.) polonicus, where the amount of hypoxanthine was rather elevated. Uric acid was also identified in some cases. The assembly efficacy of xanthine and hypoxanthine is similar to that of guanine, but xanthine significantly enhances the assembly efficacy of commercial guanine when mixed in ratio of about 1∶25. Thus, xanthine seems to be the second important component of assembly pheromone of argasid ticks.

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References

  1. Dusbábek, F., 1985. Pheromonal communication of homogamic and heterogamic populations ofArgas (Persicargas) persicus (Acarina: Argasidae). Věst. Česk. Spol. Zool., 49: 244–252.Google Scholar
  2. Gothe, R., 1987. Tick pheromones. Onderstepoort J. Vet. Res., 54: 439–442.PubMedGoogle Scholar
  3. Gothe, R. and Kraiss, A., 1982. Localization of pheromone emission and perception inArgas (Persicargas) walkerae Kaiser and Hoogstraal, 1969. Zentralbl. Veterinärmed., Reihe B, 29: 573–582.Google Scholar
  4. Gothe, R., Weck, P. and Kraiss, A., 1984. Zur pheromonal induzierten Kommunikation vonArgas (Persicargas) walkerae und biologisch-chemischen Bekämpfung durch kombinierten Einsatz eines Pheromones und Pheromon-Analogous mit Flumethrin. Zentralbl. Veterinärmed., Reihe B., 31: 161–179.Google Scholar
  5. Hamdy, B.H., 1972. Biochemical and physiological studies of certain ticks (Ixodoidea). Nitrogenous excretory products ofArgas (Persicargas) arboreus Kaiser, Hoogstraal, and Kohls and other argasid and ixodid species. J. Med. Entomol., 9: 346–350.PubMedGoogle Scholar
  6. Hamdy, B.H., 1973a. Biochemical and physiological studies of certain ticks (Ixodoidea). Cycle of nitrogenous excretion inArgas (Persicargas) arboreus Kaiser Hoogstraal, and Kohls (Argasidae). J. Med. Entomol., 10: 53–57.PubMedGoogle Scholar
  7. Hamdy, B.H., 1973b. Biochemical and physiological studies of certain ticks (Ixodoidea). Cycle of nitrogenous excretion ofHyalomma dromedarii Koch (Ixodidae). J. Med. Entomol., 10: 345–348.PubMedGoogle Scholar
  8. Hamdy, B.H. and Sidrak, W., 1982. Guanine, biosynthesis in the ticks (Acari)Dermacentor andersoni (Ixodidae) andArgas (Persicargas) arboreus (Argasidae). Fate of labelled guanine precursors. J. Med. Entomol. 19: 569–572.PubMedGoogle Scholar
  9. Hassanali, A., Nyandat, E., Obenchain, F.A., Otieno, D.A. and Galun, R., 1989. Humidity effects on response ofArgas persicus (Oken) to guanine, an assembly pheromone of ticks. J. Chem. Ecol., 15: 791–797.Google Scholar
  10. Hunter, G., 1936. On the hydrolysis of guanine. Biochem. J., 30: 1183–1188.Google Scholar
  11. Jegorov, A., Šimek, P., Tříska, J. and Dusbábek, F., 1991. Determination of purine and pyrimidine bases and nucleosides in excreta of arthropods by conventional and microbore high performance liquid chromatography and gas chromatography with mass spectrometric detector. J. Chromatogr. (in press).Google Scholar
  12. Kland, M.J. and Johnson, L.A., 1957. A kinetic study of the ultraviolet decomposition of biochemical derivatives of nucleic acid. I. Purines. J. Am. Chem. Soc., 79: 6187–6192.Google Scholar
  13. Kraiss, A., Neitz, A.W.H. and Gothe, R., 1984. The functional significance ofArgas (Persicargas) walkerae pheromones and guanine as information carriers. Zentralbl. Bakteriol. Hyg., Ser. A, 1258: 411–412.Google Scholar
  14. Leahy, M.G., Vandehey, R. and Galun, R., 1973. Assembly pheromone(s) in the soft tickArgas persicus (Oken). Nature, 246: 515–517.Google Scholar
  15. Leahy, M.G., Karuhize, G., Mango, C. and Galun, R., 1975a. An assembly pheromone and its perception in the tickOrnithodoros moubata (Murray) (Acari: Argasidae). J. Med. Entomol., 12: 284–287.PubMedGoogle Scholar
  16. Leahy, M.G., Sternberg, S., Mango, C. and Galun, R., 1975b. Lack of specificity in assembly pheromone of soft ticks (Acari: Argasidae). J. Med. Entomol., 12: 413–414.PubMedGoogle Scholar
  17. Likeš, J. and Laga, J., 1978. Basic Statistical Tables, SNTL, Praha, 488 pp. (In Czech).Google Scholar
  18. Neitz, A.W.H. and Gothe, R., 1984. Investigation into the volatility of female pheromones and the aggregation-inducing property of guanine inArgas (Persicargas) walkerae. Onderstepoort J. Vet. Res., 51: 197–201.PubMedGoogle Scholar
  19. Otieno, D.A., Hassanali, A., Obenchain, F.D., Sternberg, A. and Galun, R., 1985. Identification of guanine as an assembly pheromone of ticks. Insect. Sci. Appl., 6: 667–670.Google Scholar
  20. Sonenshine, D.E., 1985. Pheromones and other semiochemicals of the Acari. Annu. Rev. Entomol., 30: 1–28.PubMedGoogle Scholar
  21. Sonenshine, D.E., 1986. Tick pheromones: an overview. In: J.R. Sauer and J.A. Hair (Editors), Morphology, Physiology, and Behavioural Biology of Ticks. Ellis Horwood, Chichester, UK, pp. 342–360.Google Scholar
  22. Sonenshine, D.E., Silverstein, R.M. and Rechav, Y., 1982. Tick pheromone mechanisms. In: F.D. Obenchain and R. Galun (Editors), Physiology of Ticks. Pergamon Press, Oxford, pp. 439–468.Google Scholar
  23. Tatchell, R., 1964. Digestion in the tickArgas persicus Oken. Parasitology, 54: 423–440.PubMedGoogle Scholar

Copyright information

© Elsevier Science Publishers B.V. 1991

Authors and Affiliations

  • František Dusbábek
    • 1
  • Petr Šimek
    • 2
  • Alexandr Jegorov
    • 2
  • Jan Tříska
    • 2
  1. 1.Institute of ParasitologyCzechoslovak Academy of SciencesČeské BudějoviceCzechoslovakia
  2. 2.Laboratory of Analytical Chemistry, South Bohemian Biological CentreCzechoslovak Academy of SciencesČeské BudějoviceCzechoslovakia

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