World Journal of Microbiology and Biotechnology

, Volume 8, Issue 5, pp 494–499 | Cite as

Distribution of pathogenic vibrios and other bacteria in imported frozen shrimps and their decontamination by gamma-irradiation

  • H. O. Rashid
  • H. Ito
  • I. Ishigaki
Research Papers


The distribution of pathogenic vibrios and other bacteria in eight samples of imported frozen shrimps and the effect of irradiation on these bacteria were investigated. Total aerobic bacteria were at 2×104 to 4×106/g. Coliforms consisted mainly ofEnterobacter. No salmonella were detected. A total of 66 isolates, includingVibrio parahaemolyticus, V. mimicus, V. alginolyticus, V. vulnificus, V. fluvialis and a few ofListeria monocytogenes, were obtained. The gamma-radiation dose needed to reduce by 10−4 the number of vibrio isolates andAeromonas hydrophila was about 3 kGy in frozen shrimps, whereas about 3.5 kGy was required forL. monocytogenes.

Key words

Frozen shrimps gamma-irradiation microflora pathogenic vibrio 


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  1. Anon. 1976Bacteriological Analytical Manual for Foods. Washington, DC: United States Food and Drug Administration.Google Scholar
  2. Bandekar, J.R., Chander, R. & Nerkar, D.P. 1987 Radiation control ofVibrio parahaemolyticus in shrimp.Journal of Food Safety 8, 83–88.Google Scholar
  3. Baumann, P. & Schubert, R.H.W. 1984 inBergey's Manual of Systematic Bacteriology, Vol. 1, eds Kyieg, N.R. & Holt, T.G., pp. 516–547. Baltimore: Williams and Wilkins.Google Scholar
  4. Brackett, R.E. 1988 Presence and persistence ofListeria monocytogenes in food and water.Food Technology 42, April, 162–164, 178.Google Scholar
  5. Brackett, R.E. & Beuchat, L. 1990 Pathogenicity ofListeria monocytogenes grown on crabmeat.Applied and Environmental Microbiology 56, 1216–1220.Google Scholar
  6. Chan, K.-Y., Woo, M.L., Lam, L.Y. & French, G.L. 1989Vibrio parahaemolyticus and other halophilic vibrios associated with seafood in Hong Kong.Journal of Applied Bacteriology 66, 57–64.Google Scholar
  7. Cowan, S.T. & Steel, K.J. 1974Identification of Medical Bacteria, 2nd edn. Cambridge: Cambridge University Press.Google Scholar
  8. DePaola, A., Hopkins, L.H., Peeler, J., Wentz, B. & McPhearson, R.M. 1990 Incidence ofVibrio parahaemolyticus in U.S. coastal waters and oysters.Applied and Environmental Microbiology 56, 2299–2302.Google Scholar
  9. Ingham, S.C. & Potter, N.N. 1988 Survival and growth ofAeromonas hydrophila, Vibrio parahaemolyticus, andStaphylococcus aureus on cooked mince and surimis made from atlantic pollock.Journal of Food Protection 51, 634–638.Google Scholar
  10. Ito, H., Adulyatham, P., Sangthong, N. & Ishigaki, I. 1989 Effect of gamma-irradiation on frozen shrimps to reduce microbial contamination.Radiation and Physical Chemistry 34, 1009–1011.Google Scholar
  11. Ito, H., Begum, A., Kume, T. & Takehisa, M. 1986 Distribution of microorganisms in fish meals of animal feed and their disinfection by radiation.Agricultural Chemical Society of Japan 57, 9–16.Google Scholar
  12. Ito, H. & Iizuka, H. 1978 Effect of gamma-irradiation to prevent the spoilage of retainer type Kamaboko.Food Science and Technology (Japan) 25, 14–21.Google Scholar
  13. Ito, H., Sato, T. & Iizuka, H. 1976 Study of the intermediate type ofMoraxella andAcinetobacter occurring in radurized vienna sausages.Agricultural and Biological Chemistry 40, 867–873.Google Scholar
  14. Ito, H., Watanabe, H., Takahisa, M. & Iizuka, H. 1983 Isolation and identification of radiation-resistant cocci belonging to the genusDeinococcus from sewage sludges and animal feeds.Agricultural and Biological Chemistry 47, 1239–1247.Google Scholar
  15. Jones, D. & Collins, M.D. 1986 InBergey's Manual of Systematic Bacteriology, eds. Sneeth, P.A., Mair, N.S., Sharpe, M.E. & Holt, J.G. Vol. 2. pp. 1261–1281. Baltimore: Williams and Wilkins.Google Scholar
  16. Josephson, E.F. 1981 Food irradiation and sterilization.Radiation and Physical Chemistry 18, 223–239.Google Scholar
  17. Kitamura, H. 1985 Present status of contamination of pathogenic bacteria from imported fishery products.Journal of Food Microbiology (Japan) 2, 55–60.Google Scholar
  18. Lovett, J. 1988 Isolation and enumeration ofListeria monocytogenes.Food Technology 42, April, 172–175.Google Scholar
  19. Matches, J.R. & Liston, J. 1971 Radiation sensitivity ofV. parahaemolyticus.Journal of Food Science 36, 339–342.Google Scholar
  20. Venkateswaran, K., Kiyukia, C., Takaki, M., Nakano, H., Matsuda, H., Kawakami, H. & Hashimoto, H. 1989 Characterization of toxigenic vibrios isolated from the freshwater environment of Hiroshima, Japan.Applied and Environmental Microbiology 56, 2613–2618.Google Scholar
  21. West, P.A., Brayton, P.R., Bryant, T.N. & Colwell, R.R. 1986 Numerical taxonomy of vibrios isolated from aquatic environments.International Journal of Systematic Bacteriology 36, 531–543.Google Scholar
  22. Yoh, M. & Honda, T. 1988 Food poisoning by vibrios.Journal of Antibacterial and Antifungal Agents 16, 345–351.Google Scholar

Copyright information

© Rapid Communications of Oxford Ltd. 1992

Authors and Affiliations

  • H. O. Rashid
    • 1
  • H. Ito
    • 1
  • I. Ishigaki
    • 1
  1. 1.Takasaki Radiation Chemistry Research EstablishmentJapan Atomic Energy Research InstituteGunmaJapan

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