Bestimmung natürlich vorkommender steroidaler Sexualhormone (Androgene und Gestagene) in Rindfleisch

  • Mathias Hartwig
  • Sonja Hartmann
  • Hans Steinhart
Originalarbeit
Received:
Revised:

Determination of naturally occurring sex steroids (androgens and progestogens) in beef

Abstract

The content of 10 naturally occurring steroidal sex hormones (androgens and progestogens), their biosynthetic precursors and metabolites was determined in 62 samples of beef (bulls, steers, heifers). After enzymatic hydrolysis of their conjugates, the steroids were extracted from the tissue by liquid-liquid extraction and purified by solid phase extraction. The identification and quantification was carried out by GC-MS of the trimethylsilyl ethers. The progestogens progesterone and pregnenolone were quantitatively dominant (43,7 and 6,5 μg/kg respectively). The highest steroid concentrations were determined in female cattle.

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Mathias Hartwig
    • 1
  • Sonja Hartmann
    • 1
  • Hans Steinhart
    • 1
  1. 1.Institut für Biochemie und LebensmittelchemieUniversität HamburgHamburgGermany

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