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Experimental & Applied Acarology

, Volume 7, Issue 1, pp 43–58 | Cite as

Vaccination againstBoophilus microplus: Localization of antigens on tick gut cells and their interaction with the host immune system

  • D. H. Kemp
  • R. D. Pearson
  • J. M. Gough
  • P. Willadsen
Article

Abstract

Cattle have been vaccinated againstBoophilus microplus with antigens derived from partially fed female ticks. The immune response of the host lyses the gut cells of adult ticks, causing a reduction in the number, weight and reproductive capacity of engorging ticks. This response is different from the immunity that cattle acquire after repeated tick infestation. Evidence is presented that the antigens used in vaccination are located on the plasma membrane of the gut cells and it is unlikely that these antigens are secreted into the host during feeding. Vaccination using such ‘concealed’ antigens may not encounter the mechanisms of immune evasion that parasites usually demonstrate.

In-vitro assays suggest that vaccination immunity is not dependent on the need to stimulate cell-mediated responses. Immunoglobulin G alone, or with the aid of complement, is enough to damage tick gut.

The normal function of the one protein antigen isolated so far is unknown but we speculate that it serves some vital function on the cell plasma membrane.

Keywords

Immune Response Plasma Membrane Normal Function Protein Antigen Host Immune System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Agbede, R.I.S. and Kemp, D.H., 1986. Immunisation of cattle againstBoophilus microplus using extracts derived from adult female ticks: histopathology of ticks feeding on vaccinated cattle. Int. J. Parasitol., 16: 35–41.PubMedGoogle Scholar
  2. Alger, N.E. and Cabrera, E.J., 1972. An increase in death rate ofAnopheles stephensi fed on rabbits immunized with mosquito antigen. J. Econ. Entomol., 65: 165–168.PubMedGoogle Scholar
  3. Allen, J.R. and Humphreys, S.J., 1979. Immunisation of guinea pigs and cattle against ticks. Nature (London), 280: 491–493.Google Scholar
  4. Balashov, Y.S., 1972. Bloodsucking ticks (Ixodoidea). Vectors of diseases of man and animals. Misc. Publ. Entomol. Soc. Am., 8: 161–376.Google Scholar
  5. Binnington, K.C., 1978. Sequential changes in salivary gland structure during attachment and feeding of the cattle tick,Boophilus microplus. Int. J. Parasitol., 8: 97–115.PubMedGoogle Scholar
  6. Binnington, K.C. and Obenchain, F.D., 1982. Structure and function of the circulatory, nervous and neuroendocrine system of ticks. In: F.D. Obenchain and R. Galun (Editors), Physiology of Ticks. Pergamon Press, Oxford, pp. 351–398.Google Scholar
  7. Cobon, G.S., Rand, K., Moore, T., Sriskantha, A., Spring, K. and Willadsen, P., 1988. Cloning and expression of gene coding for a protective antigen from the cattle tickBoophilus microplus. Proc. Conf. Vaccines 88, 6–9 November 1988, Lorne, Australia, p. G67.Google Scholar
  8. Eggenberger, L.R., Lamoreaux, W.J. and Coons, L.B., 1988. Hemocytic encapsulation inDermacentor variabilis (Say). Proc. XVIII International Congress of Entomology, 3–9 July 1988, Vancouver, Canada. Abstract, p. 136.Google Scholar
  9. Galun, R., 1978. Research into alternative arthropod control measures against livestock pests (Part 1). In: K.C. Thompson (Editor), Proc. 1975 Workshop on the Ecology and Control of External Parasites of Economic Importance on Bovines in Latin America. CIAT, Colombia, pp. 155–161.Google Scholar
  10. Goudswaard, J., van der Donk, J.A., Noordzij, A., van Dam, R.H. and Vaerman, J.-P., 1978. Protein A reactivity of various mammalian immunoglobulins. Scand. J. Immunol., 8: 21–28.PubMedGoogle Scholar
  11. Gupta, A.P., 1986. Cellular elements in the haemolymph. In: G.A. Kerkut and L.I. Gilbert (Editors), Comprehensive Insect Physiology Biochemistry and Pharmacology. Pergamon Press, Oxford, pp. 401–451.Google Scholar
  12. Haff, L.A. and Easterday, R.L., 1978. Sephacryl gels: Physical properties and evaluation of performance in gel filtration. J. Liquid Chromatogr., 1: 811–832.Google Scholar
  13. Johnston, L.A.Y., Kemp, D.H. and Pearson, R.D., 1986. Immunisation of cattle againstBoophilus microplus using extracts derived from adult female ticks: effects of induced immunity on tick populations. Int. J. Parasitol., 16: 27–34.PubMedGoogle Scholar
  14. Kaufman, S.E., Kaufman, W.R. and Phillips, J.E., 1982. Mechanism and characteristics of coxal fluid excretion in the argasid tickOrnithodorus moubata. J. Exp. Biol., 98: 343–352.Google Scholar
  15. Kemp, D.H., Agbede, R.I.S., Johnston, L.A.Y. and Gough, J.M., 1986. Immunisation of cattle againstBoophilus microplus using extracts derived from adult female ticks: Feeding and survival of the parasite on vaccinated cattle. Int. J. Parasitol., 16: 115–120.PubMedGoogle Scholar
  16. Kerlin, R.L. and Watson, D.L., 1987. Effect of dextran sulphate on IgG subclass of antibody in efferent popliteal lymph of sheep. Immunol. Cell Biol., 65: 411–417.PubMedGoogle Scholar
  17. MacDonald, R.S., Surgeoner, A.G., Solomon, K.R. and Harris, C.R., 1983. Effect of four spray regimes on the development of permethrin and dichlorvos resistance in the laboratory, by the housefly (Diptera: Muscidae). J. Econ. Entomol., 76: 417–422.PubMedGoogle Scholar
  18. Nolan, J. and Schnitzerling, H.J., 1986. Drug resistance in arthropod parasites. In: W.C. Campbell and R.S. Rew (Editors), Chemotherapy of Parasitic Diseases. Plenum Press, New York, pp. 603–620.Google Scholar
  19. Ribeiro, J.M.C., 1989. Role of saliva in tick/host interactions. Exp. Appl. Acarol., 7: 15–20.PubMedGoogle Scholar
  20. Schlein, Y. and Lewis, C.T., 1976. Lesions in haematophagous flies after feeding on rabbits immunized with fly tissues. Physiol. Entomol., 1: 55–59.Google Scholar
  21. Slot, J.W. and Geuze, H.J., 1985. A new method of preparing gold probes for multiple-labelling cytochemistry. Europ. J. Cell Biol., 38: 87–93.PubMedGoogle Scholar
  22. Tatchell, R.J. and Moorhouse, D.E., 1968. The feeding processes of the cattle tickBoophilus microplus (Canestrini). II. The sequence of host-tissue changes. Parasitology, 58: 441–459.PubMedGoogle Scholar
  23. Walladde, S.M., Kemp, D.H. and Rice, M.J., 1079. Feeding electrograms and fluid uptake measurement of cattle tickBoophilus microplus attached on artificial membranes. Int. J. Parasitol., 9: 89–95.Google Scholar
  24. Wharton, R.H., 1976. Tick-borne livestock diseases and their vectors. 5. Acaricide resistance and alternative methods of tick control. World Anim. Rev., 20: 8–15.Google Scholar
  25. Willadsen, P., 1987. Immunological approaches to the control of ticks. Int. J. Parasitol., 17: 671–677.PubMedGoogle Scholar
  26. Willadsen, P., Kemp, D.H. and RcKenna, R.V., 1984. Bloodmeal ingestion and utilization as a component of host specificity in the tickBoophilus microplus. Z. Parasitenkd., 70: 415–420.Google Scholar
  27. Willadsen, P., McKenna, R.V. and Riding, G.A., 1988a. Isolation from the cattle tick,Boophilus microplus, of antigenic material capable of eliciting a protective response in the bovine host. Int. J. Parasitol., 18: 183–189.PubMedGoogle Scholar
  28. Willadsen, P., Riding, G.A., McKenna, R.V., Kemp, D.H. and Cobon, G.A., 1988b. Isolation of a protective antigen fromBoophilus microplus. Proc. Conf. Vaccines '88, 6–9 November 1988, Lorne, Australia, p. G67.Google Scholar
  29. Willadsen, P. and Kemp, D.H., 1988. Vaccination with ‘concealed’ antigens for tick control. Parasitol. Today, 4: 196–198.PubMedGoogle Scholar

Copyright information

© Elsevier Science Publishers B.V. 1989

Authors and Affiliations

  • D. H. Kemp
    • 1
  • R. D. Pearson
    • 1
  • J. M. Gough
    • 1
  • P. Willadsen
    • 1
  1. 1.Division of Tropical Animal Production, Long Pocket LaboratoriesCSIROIndooroopilly(Australia)

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