Modification of an in-vitro method for estimating the bioavailability of zinc and calcium from foods

  • Li -hua Shen
  • Joop Luten
  • Harry Robberecht
  • Jacques Bindels
  • Hendrik Deelstra
Original Paper

Abstract

A continuous flow in-vitro method for estimating the bioavailability of minerals and trace elements was modified. This modified method includes a simulated gastric digestion with pepsin, gradual pH change during the first 30 min of dialysis in an Amicon stirred cell, and a further 2 h of continuous dialysis accompanied by intestinal digestion with pancreatin-bile extract. The percentage of continuously dialysed minerals or trace elements (dialysability) is used to express the bioavailability. Comparison of different in-vitro methods by using the dialysability of zinc and calcium from milk- and soy-based formula samples revealed that with the modified method the results are closer to the in-vivo situation and could be used as a relative index for predicting the bioavailability of some minerals and trace elements.

Modifikation einer in-vitro-Methode zur Bestimmung der Bioverfügbarkeit von Zink und Calcium aus Lebensmitteln

Zusammenfassung

Eine continuous-flow-Methode zur in-vitro-Bestimmung der Bioverfügbarkeit von Mineralien und Spurenelementen wurde verbessert. Die Proben werden mit Pepsin in einer Dialysezelle verdaut, der pH-Wert dabei graduell verändert und bei der nachfolgenden Verdauung mit Pankreas-Galle-Extrakt die freigesetzten Mineralstoffe kontinuierlich durch Dialyse abgezogen. Der dialysierbare Anteil gilt als Maß für die Bioverfügbarkeit. Ein Vergleich der so erhaltenen Daten für Zink und Calcium aus Milch- und Soja-Formulanahrung zeigt gute Übereinstimmung mit in-vivo-Messungen. Die Methode dürfte sich zur Vorhersage der Bioverfügbarkeit für Mineralstoffe eignen.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Li -hua Shen
    • 1
  • Joop Luten
    • 2
  • Harry Robberecht
    • 1
  • Jacques Bindels
    • 3
  • Hendrik Deelstra
    • 1
  1. 1.Laboratory of Food Sciences, Department of Pharmaceutical SciencesUniversity of Antwerp (UIA)WilrijkBelgium
  2. 2.Netherlands Institute for Fisheries Research (RIVO-DLO)AB IJmuidenThe Netherlands
  3. 3.Nutricia BVHA ZoetermeerThe Netherlands

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