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Journal of Neurocytology

, Volume 24, Issue 10, pp 775–781 | Cite as

Remyelination of mouse spinal cord axons demyelinated by local injection of lysolecithin

  • N. D. Jeffery
  • W. F. Blakemore
Article

Summary

Local injections of lysolecithin are commonly used to produce areas of demyelination in the CNS. For the mouse, the demyelinating initial phase of lesion development has been described previously but the spontaneous repair which follows has not. In this study we describe the morphological sequelae which result from the injection of 2 μl of 1% lysolecithin into the spinal cord of adult mice. Lesion length was variable, extending 8mm or more in 33% of lesioned animals. By 7 days after injection very little extracellular myelin debris was detected and remyelination had commenced. Remyelination progressed rapidly so that almost all axons were invested by myelin sheaths by 23 days. Remyelination was accompanied by a prominent astrocytosis. The long lesion length and the rapidity of repair has important implications for studies designed to assess the ability of transplanted myelinogenic cells to migrate towards demyelinating lesions.

Keywords

Spinal Cord Important Implication Initial Phase Adult Mouse Myelin Sheath 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman and Hall 1995

Authors and Affiliations

  • N. D. Jeffery
    • 1
  • W. F. Blakemore
    • 1
  1. 1.Department of Clinical Veterinary MedicineCambridgeUK

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