Biotechnology Letters

, Volume 17, Issue 3, pp 305–308 | Cite as

Regioselectivity enhancement by partial purification of lipase fromAspergillus niger

  • Hao-Ping Chen
  • Kwo-Feng Hsiao
  • Shih-Hsiung Wu
  • Kung-Tsung Wang
Article

Abstract

Lipase fromAspergillus niger was partially purified by a single step of ion-exchange chromatography and two major fractions I and II could be obtained. Two substrates, peracetylated 1-methyl and 1-thioethyl β-D-glucopyranosides, were deacetylated by fraction II to give 3-OH derivatives as sole products with very high yield, respectively.

Keywords

Chromatography Purification Lipase Single Step Major Fraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1. (a)
    Faber,K., Riva,S.Synthesis,1992, 895.Google Scholar
  2. 1. (b)
    Boland. W, Frobl, C.Lorenz,U.Synthesis,991, 1048.Google Scholar
  3. 1. (c)
    Chen, C.S., Sih, C.J.Angew.Chem. Int. Eng. Ed. 1989, 28, 695.Google Scholar
  4. 2. (a)
    Cintas, P.Tetrahedron,1991, 47, 6079.Google Scholar
  5. 2. (b)
    Hanessian, S.Total Synthesis of Natural Products: The Chiron Approach, Pergamon, Oxford,1983.Google Scholar
  6. 3. (a)
    Haines, A.H.Adv. Carbohydr. Chem. Biochem.,1976, 33, 11.Google Scholar
  7. 3. (b)
    Haines, A.H.Adv. Carbohydr. Chem. Biochem.,1981, 39, 13.Google Scholar
  8. 4.
    Aspinall, G.O. in G.O. Aspinall (Ed.), in Int. Rev. Sci.,Org. Chem. Ser. Two,Butterworths, London,1976, 7, 201.Google Scholar
  9. 5. (a)
    Holla, W.Angew. Chem. Int. Ed. Engl. 1989, 28, 220.Google Scholar
  10. 5. (b)
    Therisod, M., Klibanov, A.M.J. Am. Chem. Soc. 1987, 109, 3977.Google Scholar
  11. 5. (c)
    Hene, W.J.,Sweers, H.M.,Wang, Y.F.,Wong, C.H.J.Org. Chem. 1988, 52, 4939.Google Scholar
  12. 5. (d)
    Shaw, J.F.,Klibanov, A.M.Biotecnol. Bioeng. 1987, 29, 648.Google Scholar
  13. 6.
    Hsiao, K.F., Wu, S.H., Wang, K.T.BioMed Chem. Lett. 1993,3, 2125.Google Scholar
  14. 7.
    Sweers, H.M., Wong, C.H.,J. Am. Chem. Soc. 1986,108, 6421.Google Scholar
  15. 8.
    Hennen, W. J. Sweers, H. M.; Wang, Y. F. and Wong, C. H.J. Org. Chem.,1988,53, 4939.Google Scholar

Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • Hao-Ping Chen
    • 1
  • Kwo-Feng Hsiao
    • 2
  • Shih-Hsiung Wu
    • 3
  • Kung-Tsung Wang
    • 2
    • 3
  1. 1.Department of BiochemistryChina Medical CollegeTaichungTaiwan
  2. 2.Department of ChemistryNational Taiwan UniversityTaipeiTaiwan
  3. 3.Institute of Biological Chemistry, Academia SinicaTaipeiTaiwan

Personalised recommendations