Journal of Neurocytology

, Volume 21, Issue 2, pp 94–104 | Cite as

Localization of actin in the retina of the crayfishProcambarus clarkii

  • G. S. Hafner
  • T. R. Tokarski
  • J. Kipp
Article
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Revised:
Accepted:

Summary

The distribution of actin in the retina of the crayfish was investigated at the LM level using FITC-phalloidin. Fluorescent staining was associated with the main rhabdom and eighth cell rhabdom, the zonula adherens junctions between retinula cells, and the basement membrane of the retina. EM and S1 decoration were used to confirm the presence of actin and identify its structural relationships. Phalloidin staining of the rhabdom and S1 decoration of actin filaments in the rhabdom microvilli confirmed earlier findings that actin is a component of the microvillus cytoskeleton in the crayfish. At the zonula adherens junctions, actin filaments, identified by S1 decoration, run longitudinally within the plaque of the junction. At the extreme proximal end of the rhabdom, actin filaments associated with the junctions fill each small area of retinula cell cytoplasm. In the basement membrane, EM and S1 decoration show that basilar cells contain large bundles of actin filaments which are associated with cell-matrix adherens junctions. Foot cells which lie immediately below the rhabdom also contain similar junctions and actin is tentatively identified in these cells. The functional role of actin at these various locations is discussed in relation to retinal organization in the crayfish and other invertebrates.

Keywords

Retina Basement Membrane Actin Filament Phalloidin Structural Relationship 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Chapman and Hall Ltd 1992

Authors and Affiliations

  • G. S. Hafner
    • 1
  • T. R. Tokarski
    • 1
  • J. Kipp
    • 1
  1. 1.School of OptometryIndiana UniversityBloomingtonUSA

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