Journal of Neurocytology

, Volume 18, Issue 5, pp 647–660 | Cite as

Morphometric studies on the microvasculature of pre- and paravertebral sympathetic ganglia in the adult and aged rat by light and electron microscopy

  • D. M. Baker
  • R. M. Santer
  • A. S. Blaggan
Article
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Revised:
Accepted:

Summary

Morphometric measurements have been made by light and electron microscopy on sections of perfused sympathetic ganglia from rats of 6–24 months of age with special reference to the microvascular bed. Capillaries, postcapillary venules and small venules comprised the majority of the vessels studied but small arterioles were, in addition, included in the light microscopical part of the study. Light microscopical measurements using image analysis showed that there was a decrease in the density of the microvascular bed (number of vascular profiles/area) and in the total vascular luminal area with age in both ganglia. The ratio of neurons to microvessels remained constant in the superior cervical ganglion (SCG) but decreased with age in the coeliac-superior mesenteric ganglion (CSMG). However, the distribition of microvessels in relation to individual neurons remained unchanged throughout life in both ganglia. Ultrastructural studies revealed fenestrations in 12% of SCG microvessel profiles and in 38% of CSMG microvessels at 6 months, but the percentage of fenestrated profiles in the CSMG had declined by 24 months. There were no significant differences in the number of fenestrations per fenestrated profile. The basal lamina surrounding the microvessels increased significantly (almost doubling) in thickness with age. The range and distribution of microvessel wall thicknesses, expressed as harmonic mean wall thicknesses, were comparable in both ganglia at both ages and increased linearly according to the amount of pericyte covering present. No consistently significant relationships could be discerned between the microvessel wall thickness and luminal diameter or between the wall thickness and the area available to a given volume of blood for exchange of metabolites (luminal perimeter/luminal area). We conclude that the ganglionic capillary bed is similar in both ganglia and, in old age, accommodates structural changes that occur in the ganglion by maintaining its structure and relationship to individual neurons.

Keywords

Luminal Basal Lamina Individual Neuron Sympathetic Ganglion Superior Cervical Ganglion 
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References

  1. Arvidson, B. (1979a) Distribution of protein tracers in peripheral ganglia. A light and electron microscopic study in rodents after various modes of tracer administration.Acta Universitatis Upsaliensis 344, 1–72.Google Scholar
  2. Arvidson, B. (1979b) Distribution of intravenously injected protein tracers in peripheral ganglia of adult mice.Experimental Neurology 63, 388–410.Google Scholar
  3. Baker, D. M. &Sanier, R. M. (1988a) Morphometric studies on pre- and paravertebral sympathetic neurons in the rat: changes with age.Mechanisms of Ageing and Development 42, 139–45.Google Scholar
  4. Baker, D. M. &Santer, R. M. (1988b) A quantitative study of the effects of age on the noradrenergic innervation of Auerbach's plexus in the rat.Mechanisms of Ageing and Development 42, 147–58.Google Scholar
  5. Baluk, P. &Gabella, G. (1987) Fine structure of the autonomie ganglia of the mouse pulmonary vein.Journal of Neurocytology 16, 169–84.Google Scholar
  6. Bar, T. (1978) Morphological evaluation of capillaries in different laminae of rat cerebral cortex by automatic image analysis: changes during development and aging.Advances in Neurology 20, 1–9.Google Scholar
  7. Bell, M. A. &Ball, M. J. (1981) Morphometric comparison of hippocampal microvasculature in ageing and demented people: diameters and densities.Acta Neuropatho-logica (Berlin) 53, 299–318.Google Scholar
  8. Bell, M. A. &Ball, M. J. (1985) Laminar variation in the microvascular architecture of normal human visual cortex (area 17).Brain Research 335, 139–43.Google Scholar
  9. Bell, M. A. &Weddell, A. G. M. (1984) A morphometric study of intrafascicular vessels of mammalian sciatic nerve.Muscle & Nerve 7, 524–34.Google Scholar
  10. Bergmann, L. (1943) The blood vessels of human coeliac ganglia and changes in their vascular pattern associated with age.Anatomical Record 85, 117–33.Google Scholar
  11. Burns, E. M., Kruckeberg, T. W. &Gaetano, P. K. (1981) Changes with age in cerebral capillary morphology.Neurobiology of Aging 2, 285–91.Google Scholar
  12. Deface, D. M. (1981) Morphologic study of the blood vessels of the superior cervical ganglion of the Albino rat.Acta Anatomica 109, 238–46.Google Scholar
  13. Donahue, S. &Pappas, G. (1961) The fine structure of capillaries in the cerebral cortex of the rat at various stages of development.American Journal of Anatomy 108, 331–47.Google Scholar
  14. Dunning, H. S. &Wolff, H. G. (1937) The relative vascularity of the various parts of the central and peripheral nervous system of the cat and its relation to function.Journal of Comparative Neurology 67, 433–50.Google Scholar
  15. Elfvin, L.-G., Hokfelt, T. &Goldstein, M. (1975) Fluorescence microscopical, immunohistochemical and ultrastructural studies on sympathetic ganglia of the guinea-pig, with special reference to the SIF cells and their catecholamine content.Journal of Ultrastructure Research 51, 377–96.Google Scholar
  16. Gabella, G., Trigg, P. &McPhail, H. (1988) Quantitative cytology of ganglion neurons and satellite glial cells in the superior cervical ganglion of the sheep. Relationship with ganglion neuron size.Journal of Neurocytology 17, 753–69.Google Scholar
  17. Grillo, M. A., Jacobs, L. &Comroe, J. H. (1974) A combined fluorescent histochemical and electron microscopical method for studying special monoamine containing (SIF) cells.Journal of Comparative Neurology 153, 1–14.Google Scholar
  18. Hervonen, A., Partanen, M., Helen, P., Koisti-Naho, J., Alho, H., Baker, D. M., Johnson, J. E. &Santer, R. M. (1986) The sympathetic neuron as a model of neuronal aging. InNeurohistochemistry: Modern Methods and Applications (edited byPanula, P. et al.), pp. 569–86. New York: Alan R. Liss.Google Scholar
  19. Heym, C. &Williams, T. H. (1979) Evidence for autonomic paraneurons in sympathetic ganglia of a shrew (Tupaia glis).Journal of Anatomy 129, 151–64.Google Scholar
  20. Hicks, P., Rolsten, C., Brizzee, D. &Samorajski, T. (1983) Age-related changes in rat brain capillaries.Neuro-biology of Aging 4, 69–75.Google Scholar
  21. Hunziker, O., Abdel'al, S. &Schulz, U. (1979) The aging human cerebral cortex: a stereological characterization of changes in the capillary net.Journal of Gerontology 34, 345–50.Google Scholar
  22. Hunziker, O., Frey, H. &Schulz, U. (1974) Morphometric investigations of capillaries in the brain cortex of the cat.Brain Research 65, 1–11.Google Scholar
  23. Ishikawa, H. (1959) A metrological study of the blood capillary density of the rhombencephalon, spinal cord and the peripheral nerves in the cat.Fukuoka Acta Medica 50, 4275–92.Google Scholar
  24. Jacobs, J. M. (1977) Penetration of systemically injected horseradish peroxidase into ganglia and nerves of the autonomie nervous system.Journal of Neurocytology 6, 607–18.Google Scholar
  25. Leuenberger, P. M. (1973) Ultrastructure of the ageing retinal vascular system, with special reference to quantitative and qualitative changes of capillaries basement membranes.Gerontologia 19, 1–15.Google Scholar
  26. Marcarian, H. Q. &Smith, R. D. (1968) A quantitative study on the vasa nervorum in the ulnar nerve of cats.Anatomical Record 161, 105–10.Google Scholar
  27. Mancardi, G. L., Perdelli, F., Rivano, C., Leonardi, A. &Bugiani, O. (1980) Thickening of the basement membrane of cortical capillaries in Alzheimer's disease.Acta Neuropathologica (Berlin) 49, 79–83.Google Scholar
  28. Matthews, M. R. &Raisman, G. (1969) The ultrastructure and somatic efferent synapses of small granule-containing cells in the superior cervical ganglion.Journal of Anatomy 105, 255–82.Google Scholar
  29. McDonald, D. M. (1983) A morphometric analysis of blood vessels and perivascular nerves in the rat carotid body.Journal of Neurocytology 12, 155–99.Google Scholar
  30. Meier-Ruge, W., Hunziker, O., Schulz, U., Tobler, H. -J. &Schweizer, A. (1980) Stereological changes in the capillary network and nerve cells of the aging human brain.Mechanisms of Ageing and Development 14, 233–43.Google Scholar
  31. Partanen, M., London, E. D. &Rapoport, S. I. (1982) Glucose utilization in sympathetic ganglia of male Fischer-344 rats at different ages.Journal of the Autonomic Nervous System 5, 391–8.Google Scholar
  32. Reis, D. J., Ross, R. A. &Joh, T. H. (1977) Changes in the activity and amounts of enzymes synthesizing catechola-mines and acetylcholine in brain, adrenal medulla and sympathetic ganglia of aged rat and mouse.Brain Research 136, 465–74.Google Scholar
  33. Rosenstein, J. M. &Brightman, M. W. (1983) Circumventing the blood-brain barrier with autonomie ganglion transplants.Science 221, 879–81.Google Scholar
  34. Santer, R. M. (1979) Fluorescence histochemical evidence for decreased noradrenaline synthesis in sympathetic neurones of aged rats.Neuroscience Letters 15, 177–80.Google Scholar
  35. Santer, R. M. &Kabeer, U. (1987) Morphometric analysis of alkaline phosphatase stained capillaries in rat sympathetic ganglia: changes during development and ageing.Experimental Brain Research Series 16, 278–81.Google Scholar
  36. Santer, R. M., Lu, K. -S., Lever, J. D. &Presley, R. (1975) A study of the distribution of chromaffin-positive (CH+) and small intensely fluorescent (SIF) cells in sympathetic ganglia of the rat at various ages.Journal of Anatomy 119, 589–99.Google Scholar
  37. Santer, R. M., Partanen, M. &Hervonen, A. (1980) Glyoxylic acid fluorescence and ultrastructural studies of neurones in the coeliac-superior mesenteric ganglion of aged rats.Cell and Tissue Research 211, 475–85.Google Scholar
  38. Tsubaki, S., Brightman, M. W., Nakagawa, H., Owens, E. &Blasberg, R. G. (1987) Local blood flow and vascular permeability of autonomie ganglion-transplants in the brain.Brain Research 424, 71–83.Google Scholar

Copyright information

© Chapman and Hall Ltd. 1989

Authors and Affiliations

  • D. M. Baker
    • 1
  • R. M. Santer
    • 1
  • A. S. Blaggan
    • 1
  1. 1.Department of AnatomyUniversity of Wales College of CardiffCardiffUK

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