Biotechnology Letters

, Volume 5, Issue 8, pp 531–536

The nature of inhibitory materials present in pretreated lignocellulosic substrates which inhibit the enzymatic hydrolysis of cellulose

  • Mary Mes-Hartree
  • J. N. Saddler


Wheat straw and aspen wood chips were pretreated by steam explosion and the various fractions were assayed for the presence of materials which inhibited the enzymatic hydrolysis of the cellulose component of the lignocellulosic substrates to glucose. The inhibitory material could be removed from all of the fractions by simple water extraction. The inhibitory substances were shown to primarily inhibit the B-glucosidase component of the cellulase complex of Trichoderma harzianum E58. The furan derivatives, furfural and hydroxymethyl furfural were not inhibitory at concentrations normally found in steam exploded lignocellulosic substrates.


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  1. Andren, R.K., Mandes, M.H., Medeiros, J.E. (1975) Applied Polymer Symp. No. 29 ed T.E. Timell 205–219Google Scholar
  2. Chahal, D.S., McGuire, S., Pikor, H. and Noble, G. (1982) Biomass2, 127–137Google Scholar
  3. Jurasek, L. (1979) Dev. Ind. Microbiol20, 177–183Google Scholar
  4. Marsden, W.L., Gray, P.P. and Dunn, N.W. (1982) Biotechnol. Letts.4 589–594Google Scholar
  5. Mes-Hartree, M., Hogan, C., Hayes, R.D. and Saddler, J.N. (1983) Biotechnol. Letts.5 101–106Google Scholar
  6. Miller, G.L. (1959) Anal Chem31, 426–428Google Scholar
  7. Puls, J. and Dietrichs, H.H. (1980) Proc. of Int. Conf. Biomass, Brighton, England, Nov., 348–353Google Scholar
  8. Raabo, E. and Terkildsen, T.C. (1960) Scan. J. Clin. Lab. Invest.12 420–406Google Scholar
  9. Saddler, J.N. (1982) Enzyme Microl Technol4 414–418Google Scholar
  10. Saddler, J.N. and Brownell, H.H. (1982) Proc. Int. Symp. on Ethanol from Biomass. Winnipeg, Canada Oct. 206–230Google Scholar
  11. Saddler, J.N., Brownell, H.H., Clermont, L.P. and Levitin, N. (1982) Biotech Bioeng.24, 1389–1402Google Scholar
  12. Sinitsyn, A.P., Clesceri, L.S. and Bungay, H.R. (1982). Appl. Biochem. and Biotechnol7 455–458Google Scholar
  13. Su, T.-M, Lamed, R.J., Lobos, J., Brennan, M., Smith, J.F., Tabor, D. and Brooks, R. (1980) Final report for U.S. Dept. of Energy for period Dec. 1, 1979–Dec. 31, 1980. SERI/TR-8271-1-77Google Scholar
  14. Taylor, J.D. (1980) Proc. of Int. Conf. Biomass, Brighton, England. Nov. 330–336.Google Scholar
  15. Yu, E.K.C., Levitin, N. and Saddler, J.N. (1982) Biotechnol Letts4 741–746.Google Scholar

Copyright information

© Kluwer Academic Publishers 1983

Authors and Affiliations

  • Mary Mes-Hartree
    • 1
  • J. N. Saddler
    • 1
  1. 1.Biotechnology GroupForintek Canada Corp.OttawaCanada

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