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Theoretical and Applied Genetics

, Volume 87, Issue 3, pp 347–352 | Cite as

Male-sterile chicory cybrids obtained by intergeneric protoplast fusion

  • C. Rambaud
  • J. Dubois
  • J. Vasseur
Article

Abstract

Male-sterile chicory plants were obtained by fusion of chicory mesophyll protoplasts and hypocotyl protoplasts derived from male-sterile sunflower plants. The protoplasts of both species were fused by the PEG method and the products were selected manually and cultivated at very low density in a liquid medium. Three to twenty percent of the heterokaryocytes divided and evolved into microcalli, then into calli where budding could be induced. The mitochondrial genome of ten male-sterile or totally sterile plants was studied. Restriction endonuclease profiles of mitochondrial DNA and molecular hybridization with specific genes of the mitochondrial genome used as probes indicated that mitochondrial DNA rearrangement had occurred between sunflower and chicory and the intensity of the rearrangements correlated with the degree of sterility of the different plants.

Key words

Cichorium intybus Protoplast fusion Cytoplasmic male sterility Mitochondrial DNA 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • C. Rambaud
    • 1
  • J. Dubois
    • 1
  • J. Vasseur
    • 1
  1. 1.Laboratoire de Physiologie Cellulaire et Morphogenèse Végétales, Université des Sciences et Technologies de Lille-Flandres-ArtoisVilleneuve d'Ascq cedexFrance

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