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Journal of Neurocytology

, Volume 22, Issue 9, pp 735–742 | Cite as

Thirty years of synaptosome research

  • V. P. Whittaker
Article

Summary

Detached synapses (synaptosomes), first isolated by the author in 1958 and identified as such in 1960, are sealed presynaptic nerve terminals often with a portion of the target cell — sometimes amounting to a complete dendritic spine — adhering to their external surface. They can be prepared in high yield from brain tissue and also in decreasing yield from spinal cord, retina, sympathetic ganglia, myenteric plexus and electric organs. They are sealed structures which, under metabolizing conditions, respire, take up oxygen and glucose, extrude Na+, accumulate K+, maintain a normal membrane potential and, on depolarization, release transmitter in a Ca2+-dependent manner. They thus provide an excellent preparation with which to investigate synaptic function without the complications encountered with synapsesin situ. They also serve as the parent fraction for preparations of synaptic vesicles and other synaptic components.

Keywords

Spinal Cord Retina Membrane Potential External Surface Dependent Manner 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman and Hall Ltd 1993

Authors and Affiliations

  • V. P. Whittaker
    • 1
  1. 1.Arbeitsgruppe Neurochemie, Anatomisches InstitutJohannes Gutenberg-Universität MainzMainzGermany

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