Advertisement

Planta

, Volume 188, Issue 1, pp 106–114 | Cite as

Spectral-dependence of light-inhibited hypocotyl elongation in photomorphogenic mutants ofArabidopsis: evidence for a UV-A photosensor

  • Jeff C. Young
  • Emmanuel Liscum
  • Roger P. Hangarter
Article

Abstract

Photon fluence rate-response curves at different wavelengths were generated for the light-induced inhibition of hypocotyl elongation in seedlings of wildtype and photomorphogenic mutants ofArabidopsis thaliana. (L.) Heynh. Treatment of wild-type seedlings with continuous low-fluence-rate light (< 1.0 μmol photons · m−2 · s−1) induced some inhibition of hypocotyl elongation at all wavelengths tested, with maximum inhibition in blue light. At higher fluence rates, inhibition reached a maximum of 70–80% in UV-A, blue, and far-red light. Fluence rate-response curves for seedlings ofblu1, a blue light-response mutant, showed a specific reduction in their response to blue light, but their response to UV-A, red, and far-red light was similar to that in wild-type seedlings. In contrast, the phytochromedeficient mutanthy6 showed a loss of response to lowfluence-rate light at all wavelengths, as well as to highfluence-rate far-red light. However,hy6 seedlings retained sensitivity to high-fluence-rate blue and UV-A light. The data support the conclusion that blue-lightand phytochrome-dependent photosensory systems regulate hypocotyl elongation independently and in an additive manner. Furthermore, hypocotyl inhibition in wild-type,blul, hy6 andblul-hy6 double mutants was indistinguishable in UV-A light, whereas marked differences were observed at other wavelengths, indicating the involvement of a third photosensory system with an absorption maximum in the UV-A.

Key words

Arabidopsis Photomorphogenesis Blue/UV-A light Hypocotyl elongation Phytochrome 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adamse, P., Jaspers, P.A.P.M., Bakker, J.A., Wesselius, J.C., Heeringa, G.H., Kendrick, R.E., Koornneef, M. (1988a) Photophysiology of a tomato mutant deficient in labile phytochrome. J. Plant Physiol. 127, 481–491Google Scholar
  2. Adamse, P., Kendrick, R.E., Koornneef, M. (1988b) Photomorphogenic mutants of higher plants. Photochem. Photobiol.48, 833–841Google Scholar
  3. Ballare, C.L., Casal, J.J., Kendrick, R.E. (1991) Responses of lightgrown wild-type and longhypocotyl mutant cucumber seedlings to natural and simulated shade light. Photochem. Photobiol.54, 819–826Google Scholar
  4. Baskin, T.B., Iino, M. (1987) An action spectrum in the blue and ultraviolet for phototropism inAlfalfa. Photochem. Photobiol.46, 127–136Google Scholar
  5. Beggs, C.J., Holmes, M.G., Jabben, M., Schäfer, E. (1980) Action Spectra for the inhibition of hypocotyl growth by continuous irradiation in light and dark grownSinapis alba L. seedlings. Plant Physiol.80, 615–618Google Scholar
  6. Brodhun, B., Hader, D.-P. (1990) Photoreceptor proteins and pigments in the paraflagellar body of the flagellateEuglena gracilis. Photochem. Photobiol.52, 865–871Google Scholar
  7. Chory, J., Peto, C.A., Ashbaugh, M., Saganich, R., Pratt, L., Ausubel, F. (1989) Different roles for phytochrome in etiolated and green plants deduced from characterization ofArabidopsis thaliana mutants. Plant Cell1, 867–880PubMedGoogle Scholar
  8. Coohill, T.P. (1989) Ultraviolet action spectra (280 to 380 nm) and solar effectiveness spectra for higher plants. Photochem. Photobiol.50, 451–457Google Scholar
  9. Dehesh, K., Tepperman, J., Christensen, A.H., Quail, P.H. (1991)phyB is evolutionarily conserved and constititively expressed in rice seedling shoots. Mol. Gen. Genet.225, 305–313PubMedGoogle Scholar
  10. Drumm-Herrel, H., Mohr, H. (1981) A novel effect of UV-B in a higher plant (Sorgum vulgare). Photochem. Photobiol.33, 391–398Google Scholar
  11. Evans, J.T., Hendricks, S.B., Borthwick, H.A. (1965) The role of light in supressing hypocotyl elongation in lettuce andPetunia. Planta64, 201–218Google Scholar
  12. Furuya, M. (1989) Molecular properties of hypocotyl elongation in de-etiolatedCucumis sativus L. The end of day response to phytochrome. Planta164, 264–271Google Scholar
  13. Galland, P., Senger, H. (1988a) The role of flavins as photoreceptors. J. Photochem. Photobiol.1, 277–294Google Scholar
  14. Galland, P., Senger, H. (1988b) The role of pterins in the photoreception and metabolism of plants. Photochem. Photobiol.48, 811–820Google Scholar
  15. Galland, P., Keiner, P., Dörmemann, D., Senger, H., Brodhun, B., Häder, D.-P. (1990) Pterin- and flavin-like fluorescence associated with isolated flagella ofEuglena gracilis. Photochem. Photobiol.51, 675–680Google Scholar
  16. Giese, A.C., (1968) Ultraviolet action spectra in perspective: with special reference to mutation. Photochem. Photobiol.8, 527–546PubMedGoogle Scholar
  17. Häder, D.-P., Brodhun, B. (1991) Effects of ultraviolet radiation on the photoreceptor proteins and pigments in the paraflagellar body of the flagellate,Euglene gracilis J. Plant Physiol.137, 641–646Google Scholar
  18. Hartmann, K.M. (1967) Ein Wirkungsspektrum der Photomorphogenese unter Hochenergiebedingungen; seine Interpretation auf der Basis des Phytochroms (Hypokotylwachstumshemmung beiLactuna sativa L.). Z. Naturforsch.22, 1172–1175Google Scholar
  19. Hohl, N., Galland, P., Senger, H. (1992a) Altered pterin patterns in photobehavioral mutants ofPhycomyces blakesleeanus. Photochem. Photobiol.55, 239–245PubMedGoogle Scholar
  20. Hohl, N., Galland, P., Senger, H. (1992a) Altered flavin patterns in photobehavioral mutants ofPhycomyces blakesleeanus. Photochem. Photobiol.55, 247–255PubMedGoogle Scholar
  21. Holmes, M.G., Schafer, E. (1981) Action spectra for changes in the “high irradiance reaction” in hypocotyls ofSynapsis alba L. Planta153, 267–272Google Scholar
  22. Jabben, M., Deitzer, G.F. (1979) Effects of the herbicide SAN 9789 on photomorphogenic responses. Plant Physiol.63, 481–485Google Scholar
  23. Jorns, M.S., Wang, B., Jordan, S.P., Chanderkar, L.P. (1990) Chromophore function and interaction inEscherichia coli DNA photolyase: Reconstitution of the apoenzyme with pterin and/or flavin derivatives. Biochemistry29, 552–561PubMedGoogle Scholar
  24. Jose, A.M., Vince-Prue, D. (1977) Action spectra for the inhibition of growth in radish hypocotyls. Planta136, 131–134Google Scholar
  25. Koornneef, M., Rolff, E., Spruit, C.J.P. (1980) Genetic control of light-induced hypocotyl elongation inArabidopsis thaliana (L.) HEYNH. Z. Pflanzenphysiol.100, 147–160Google Scholar
  26. Lercari, B., Sodi, F, di Paola, M.L. (1990) Photomorphogenic responses to UV radiation: Involvement of phytochrome and UV photoreceptors in the control of hypocotyl elongation inLycopersicon esculentum. Physiol. Plant.79, 668–672Google Scholar
  27. Liscum, E., Hangarter, R.P. (1991)Arabidopsis Mutants lacking blue light-dependent inhibition of hypocotyl elongation. Plant Cell.3, 685–694PubMedGoogle Scholar
  28. Massey, V., Palmer, G. (1966) On the existence of spectrally distinct classes of flavoprotein semiquinones. A new method for the quantitative production of flavoprotein semiquinones. Biochemistry5, 3181–3189PubMedGoogle Scholar
  29. Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant.15, 473–497Google Scholar
  30. Nagatani, A., Chory, J., Furuya, M. (1991) Phytochrome B is not detectable in thehy3 mutant ofArabidopsis, which is deficient in responding to end-of-day far-red light treatments. Plant Cell Physiol.32, 1119–1122Google Scholar
  31. Orbović, V., Poff, K. (1991) Role of carotenoids in first positive phototropism of etiolatedArabidopsis thaliana seedlings. Plant Physiol.96(S), 119Google Scholar
  32. Parks, B.M., Shanklin, J., Koornneef, M, Kendrick, R.E., Quail, P.H. (1989) Immunochemically detectable phytochrome is present at normal levels but is photochemically nonfunctional in thehy1 andhy2 long hypocotyl mutants ofArabidopsis thaliana. Plant Mol. Biol.12, 425–437Google Scholar
  33. Parks, B.M., Quail, P.H. (1991) Phytochrome-deficienthy1 andhy2 long hypocotyl mutants ofArabidopsis are defective in phytochrome biosynthesis. Plant Cell3, 1177–1186PubMedGoogle Scholar
  34. Presti, D., Hsu, W.J., Delbruck, M. (1977) Phototropism inPhycomyces mutants lacking β-carotene. Photochem. Photobiol.26, 403–405Google Scholar
  35. Quail, P.H. (1991) Phytochrome: A light-activated molecular switch that regulates plant gene expression. Annu. Rev. Genet.25, 389–409PubMedGoogle Scholar
  36. Sharrock, R.A., Quail, P.H. (1989) Novel phytochrome sequences inArabidopsis thaliana: structure, evolution, and differential expression of a plant regulatory photoreceptor family. Genes Dev.3, 1745–1757PubMedGoogle Scholar
  37. Shropshire, W. Jr., (1972) Action spectroscopy. In: Phytochrome pp 159–181, Mitrakos, K., Shropshire W., Jr., eds. Academic Press, LondonGoogle Scholar
  38. Smith, H., Whitelam, G.C. (1990) Phytochrome, a family of photoreceptors with multiple physiological roles. Plant Cell Environ.13, 695–708Google Scholar
  39. Somers, D.E., Sharrock, R.A., Tepperman, J.M., Quail, P.H. (1991) Thehy3 long hypocotyl mutant ofArabidopsis is deficient in phytochrome B. Plant Cell3, 1263–1274PubMedGoogle Scholar
  40. Vierstra, R., Poff, K. (1981) Role of carotenoids in the phototropic response of corn seedlings. Plant Physiol.68, 798–801Google Scholar
  41. Wang, B., Jordan, S.P., Jorns, M.S. (1988) Identification of a pterin derivative inEscherichia coli DNA photolyase. Biochemistry27, 4222–4226PubMedGoogle Scholar
  42. Wang, Y.-C., Stewart, S.J., Cordonnier, M.-M., Pratt, J.H. (1991)Avena sativa L. contains three phytochromes, only one of which is abundant in etiolated tissue. Planta184, 96–104Google Scholar
  43. Whitelam, G.C., Smith, H. (1991) Retention of phytochromemediated shade avoidance responses in phytochrome-deficient mutants ofArabidopsis, cucumber and tomato. J. Plant Physiol.139, 119–125Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Jeff C. Young
    • 1
  • Emmanuel Liscum
    • 1
  • Roger P. Hangarter
    • 1
  1. 1.Department of Plant BiologyOhio State UniversityColumbusUSA

Personalised recommendations