Journal of Neurocytology

, Volume 12, Issue 6, pp 939–948 | Cite as

A morphometric Golgi analysis of the Purkinje cell dendritic tree after long-term alcohol consumption in the adult rat

  • M. A. Tavares
  • M. M. Paula-Barbosa
  • E. G. Gray
Article

Summary

Applying a morphometric analysis, the effects of chronic alcohol consumption on Golgi-impregnated Purkinje cells from the rat cerebellar cortex were evaluated. Alcohol-fed animals received ethanol up to 1, 3, 6, 12 and 18 months and controls were given the same amount of food and an aqueous solution with sucrose replacing the ethanol isocalorifically. The following parameters of Purkinje cell dendritic trees were studied: dendritic field area, branching density, total branch length, spine density and total number of spines. Results showed a progressive decrease of all parameters in alcohol-fed animals. Significant differences were found in all rats after 12 months of alcohol intake. The reduction of the dendritic network was found to be related to a drop in mean total number of dendritic branches after 6 months of alcohol consumption. It is concluded that long-term alcohol consumption induces a decrease of the synaptic receptive area of Purkinje cells which may well lead to functional changes in the cerebellum.

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References

  1. Berry, M., Hollingworth, J., Anderson, E. M. &Flinn, R. M. (1975) Application of network analysis to the study of the branching patterns of dendritic fields. InAdvances in Neurology, Vol. 12, Physiology and Pathology of Dendrites (edited byKreutzberg, G. W.), pp. 217–45. New York: Raven Press.Google Scholar
  2. Chen, S. &Hillman, D. E. (1982) Marked reorganization of Purkinje cell dendrites and spines in adult rat, following vacating of synapses due to deafferentation.Brain Research 245, 131–5.Google Scholar
  3. Cowan, W. H. (1970) Anterograde and retrograde transneuronal degeneration in the central and peripheral nervous system. InContemporary Research Methods in Neuroanatomy (edited byNauta, W. J. H. &Ebbesson, S. O. E.), pp. 217–51. Berlin, Heidelberg, New York: Springer-Verlag.Google Scholar
  4. Freund, G. (1973) Chronic central nervous system toxicity of alcohol.Annual Review of Pharmacology 13, 217–27.Google Scholar
  5. Kalant, K. (1975) Direct effects of ethanol on the nervous system.Federation Proceedings 34, 1930–41.Google Scholar
  6. Matthews, M. R. &Powell, T. P. S. (1962) Some observations on transneuronal cell degeneration in the olfactory bulb of the rabbit.Journal of Anatomy 96, 89–102.Google Scholar
  7. Palay, S. L. &Chan-Palay, V. (1974)Cerebellar Cortex-Cytology and Organization. Berlin, Heidelberg, New York: Springer-Verlag.Google Scholar
  8. Pentney, R. J. (1982) Quantitative analysis of ethanol effects on Purkinje cell dendritic tree.Brain Research 249, 397–401.Google Scholar
  9. Pysh, J. J., Perkins, R. E. &Beck, L. S. (1979) The effect of postnatal undernutrition on the development of the mouse Purkinje cell dendritic tree.Brain Research 169, 165–70.Google Scholar
  10. Riley, J. N. (1977) Alterations in dendritic morphology following chronic alcohol consumption: a Golgi analysis. PhD dissertation, University of Florida.Google Scholar
  11. Riley, J. N. &Walker, D. W. (1978) Morphological alterations in hippocampus after long-term alcohol consumption in mice.Science 201, 646–8.Google Scholar
  12. Rogers, J., Siggins, G. R., Schulman, J. A. &Bloom, F. E. (1980) Physiological correlates of ethanol intoxication, tolerance and dependence in rat cerebellar Purkinje cells.Brain Research 196, 183–98.Google Scholar
  13. Tavares, M. A. &Paula-Barbosa, M. M. (1982) Alcohol-induced granule cell loss in the cerebellar cortex of the adult rat.Experimental Neurology 78, 574–82.Google Scholar
  14. Tavares, M. A. &Paula-Barbosa, M. M. (1983a) Lipofuscin granules in Purkinje cells after long-term alcohol consumption.Alcoholism: Clinical and Experimental Research. In press.Google Scholar
  15. Tavares, M. A. &Paula-Barbosa, M. M. (1983b) Mitochondrial changes in rat Purkinje cells after prolonged alcohol consumption. A morphologic assessment.Journal of Submicroscopic Cytology 15, 713–20.Google Scholar
  16. Tewari, S., Fleming, E. W. &Noble, E. P. (1975) Alterations in brain RNA metabolism following chronic alcohol ingestion.Journal of Neurochemistry 24, 561–9.Google Scholar
  17. Underwood, E. E. (1970)Quantitative Stereology, pp. 48–71. Massachusetts, London: Addison-Wesley.Google Scholar
  18. Valverde, F. (1970) The Golgi method: a tool for comparative structural analysis. InContemporary Research Methods in Neuroanatomy (edited byNauta, W. J. H. &Ebbesson, S. O. E.), pp. 12–31. Berlin, Heidelberg, New York: Springer-Verlag.Google Scholar
  19. Victor, M. &Adams, R. D. (1961) On the etiology of the alcoholic neurologic diseases with special reference to the role of nutrition.American Journal of Nutrition 9, 379–97.Google Scholar
  20. Walker, D. W., Barnes, D. E., Zornetzer, S. F., Hunter, B. E. &Kubanis, P. (1980) Neuronal loss in hippocampus induced by prolonged ethanol consumption in rats.Science 209, 711–3.Google Scholar
  21. Walker, D. W., Hunter, B. E. &Abraham, W. C. (1981) Neuroanatomical and functional deficits subsequent to chronic ethanol administration in animals.Alcoholism: Clinical and Experimental Research 5, 267–82.Google Scholar
  22. Weiss, G. M. &Pysh, J. J. (1978) Evidence for loss of Purkinje cell dendrites during late development: a morphometric Golgi analysis in the mouse.Brain Research 154, 219–30.Google Scholar

Copyright information

© Chapman and Hall Ltd 1983

Authors and Affiliations

  • M. A. Tavares
    • 1
  • M. M. Paula-Barbosa
    • 1
  • E. G. Gray
    • 2
  1. 1.Department of AnatomyOporto School of MedicineOportoPortugal
  2. 2.National Institute for Medical ResearchLondonUK

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