Neuroscience and Behavioral Physiology

, Volume 16, Issue 4, pp 285–289

Nuclease sensitivity and methylation of liver chromatin DNA in rats in initial period of effect of glucocorticoids

  • G. A. Romanov
  • E. N. Zhavoronkova
  • S. V. Savel'ev
  • B. F. Vanyushin
Article

Conclusions

  1. 1.

    Sensitivity of chromatin DNA of rat liver to DNase I and the level of its methylation do not change noticeably 3.5 h after administration of an inducing dose of hydrocortisone to animals.

     
  2. 2.

    Loci activated by glucocorticoids must initially possess potentially active conformation sensitive to DNase I.

     

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. 1.
    G. A. Romanov, N. A. Romanova, V. B. Rozen, et al., Mol. Biol.,15, No. 4, 857–874 (1981).Google Scholar
  2. 2.
    F. Payvar, O. Wrange, J. Carlstedt-Duke, et al., Proc. Natl. Acad. Sci. USA,78, 6628–6632 (1981).Google Scholar
  3. 3.
    J. G. Compton, W. T. Schrader, and B. W. O'Malley, Biochem. Biophys. Res. Commun.,105, 96–104 (1982).Google Scholar
  4. 4.
    E. R. Mulvihill, J.-P. LePennec, and P. Chambon, Cell,28, 621–632 (1982).Google Scholar
  5. 5.
    A. P. Kuz'menko, V. S. Dashkevich, G. A. Romanov, et al., Dokl. Akad. Nauk SSSR,272, No. 3, 739–741 (1983).Google Scholar
  6. 6.
    R. T. Simpson, in: Gene Amplification and Analysis, Vol. 2, New York (1981), pp. 347–368.Google Scholar
  7. 7.
    B. F. Vanyushin, Usp. Biol. Khimii,24, 15 (1983).Google Scholar
  8. 8.
    G. Schütz and P. Feigelson, Anal. Biochem.,46, 149–155 (1972).Google Scholar
  9. 9.
    G. A. Romanov, N. A. Sokolova, V. B. Rozen, et al., Biokhimiya, No. 12, 2140–2149 (1976).Google Scholar
  10. 10.
    A. S. Spirin, Biokhimiya, No. 5, 656–662 (1958).Google Scholar
  11. 11.
    K. Burton, Methods Enzymol.,12, 163 (1968).Google Scholar
  12. 12.
    G. Schütz, L. Killewich, G. Chen, et al., Proc. Natl. Acad. Sci. USA,75, 1017–1020 (1975).Google Scholar
  13. 13.
    W. G. Roewekamp. E. Hofer, and C. E. Sekeris, Eur. J. Biochem.,70, 259–268 (1976).Google Scholar
  14. 14.
    F. Kenney and F. Kull, Proc. Natl. Acad. Sci. USA,50, 493 (1963).Google Scholar
  15. 15.
    H. Grote, J. Voigt, and C. E. Sekeris, Biochem. J.,212, 305–312 (1983).Google Scholar
  16. 16.
    G. S. Arbuzova, I. M. Gryaznova, T. M. Morozova, et al., Mol. Biol.,2, No. 3, 308–312 (1968).Google Scholar
  17. 17.
    R. I. Salganik, T. M. Morozova, and N. A. Zakharov, Biochim. Biophys. Acta, 174, 755–757 (1969).Google Scholar
  18. 18.
    L. K. Johnson and J. D. Baxter, J. Biol. Chem.,253, 1991–1997 (1978).Google Scholar
  19. 19.
    V. V. Adler, M. A. Krasil'nikov, G. Yu. Bochkarev, et al., Biokhimiya, No. 6, 915–920 (1982).Google Scholar
  20. 20.
    N. A. Dudareva, V. S. Dashkevich, and R. I. Salganik, Biokhimiya, No. 7, 1305–1311 (1980).Google Scholar
  21. 21.
    J. C. Gasson, T. Ryden, and S. Bourgeois, Nature,302, 621–623 (1983).Google Scholar
  22. 22.
    S. J. Compere and R. D. Palmiter, Cell,25, 233–240 (1981).Google Scholar
  23. 23.
    J.-N. Lapeyre, A. L. Maizel, and F. F. Becker, Biochem. Biophys. Res. Commun.,95, 630–637 (1980).Google Scholar
  24. 24.
    R. D. Palmiter, E. R. Mulvihill, G. S. Mcknight, et al., Cold Spring Harbor Symp. Quant. Biol.,42, 639–647 (1978).Google Scholar
  25. 25.
    A. Garel and R. Axel, Proc. Natl. Acad. Sci. USA,74, 4867–4871 (1977).Google Scholar
  26. 26.
    M. T. Kuo, J. L. Mandel, and P. Chambon, Nucleic Acids Res.,7, 2106–2113 (1979).Google Scholar
  27. 27.
    A. F. Wilks, P. J. Cozens, I. W. Mattaj, et al., Proc. Natl. Acad. Sci. USA,79, 4252–4255 (1982).Google Scholar
  28. 28.
    G. J. Dimitriadis and J. R. Tata, Biochem. J.,202, 491–497 (1982).Google Scholar
  29. 29.
    J. L. Williams and J. R. Tata, Nucleic Acids Res.,11, 1151–1166 (1983).Google Scholar
  30. 30.
    V. B. Rozen and A. N. Smirnov, Receptors and Steroid Hormones [in Russian], Moscow (1981), pp. 65–76.Google Scholar

Copyright information

© Plenum Publishing Corporation 1986

Authors and Affiliations

  • G. A. Romanov
    • 1
  • E. N. Zhavoronkova
    • 1
  • S. V. Savel'ev
    • 1
  • B. F. Vanyushin
    • 1
  1. 1.A. N. Belozerskii Interdepartmental Laboratory of Molecular Biology and Bioorganic ChemistryMoscow State UniversityMoscow

Personalised recommendations