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Transport and utilization of ferrioxamine-E-bound iron inErwinia herbicola (Pantoea agglomerans)

Summary

We have analyzed ferrioxamine-E-mediated iron uptake and metabolization inErwinia herbicola K4 (Pantoea agglomerans) by means of in vivo Mössbauer spectroscopy and radioactive labeling techniques. A comparison of cell spectra with the spectrum of ferrioxamine clearly demonstrates that ferrioxamine E is not accumulated in the cell, indicating a fast metal transfer. Only two major components of iron metabolism can be detected, a ferric and a ferrous species. At 30 min after uptake, 86% of the internalized metal corresponded to a ferrous ion compound and 14% to a ferric iron species. Metal transfer apparently involves a reductive process. With progressing growth, the oxidized species of the two major proteins becomes dominant. The two iron metabolites closely resemble species previously isolated fromEscherichia coli. These components of iron metabolism differ from bacterio-ferritin, cytochromes and most iron-sulfur proteins. All other iron-containing cellular components are at least one order of magnitude lower in concentration. We suggest that the ferrous and ferric iron species correspond to two different oxidation states of a low-molecular mass protein.

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Correspondence to Berthold F. Matzanke.

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Matzanke, B.F., Berner, I., Bill, E. et al. Transport and utilization of ferrioxamine-E-bound iron inErwinia herbicola (Pantoea agglomerans). Biol Metals 4, 181–185 (1991). https://doi.org/10.1007/BF01141312

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Key words

  • Erwinia herbicola (Pantoea agglomerans)
  • Ferrioxamine E
  • In vivo M6ssbauer spectroscopy
  • Iron metabolism
  • Iron transport
  • Siderophores