A simple method is described for the electrofusion of plant protoplasts. Protoplasts were aggregated in a radio-frequency field (10 V RMS, 0.5 MHz) for 15-30 s with an inter-electrode distance of J mm. They were then fused with a 300-V DC pulse. The protoplasts were able to divide after this treatment. A trans-ferrable electrode permitted electrofusion of l-ml volumes of culture in standard tissue-culture dishes in about 20 s.
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Barton KA & Winston JB (1983) Science219, 671–676.
Keller WA & Melchers G (1973) Z. Naturforsch.28c, 737–741.
Kao KN & Michayluk MR (1974) Planta115, 355–367.
Evans DA (1938) Biotechnology1, 253–261.
Senda M, Takeda J, Abe S & Nakamura T (1979) Plant Cell Physiol.20, 1441–1443.
Zimmermann U & Scheurich P (1981) Planta151, 26–32.
Bates GW, Gaynor JJ & Shekhawat NS (1983) Plant Physiol.72, 1110–1113.
Motoyoshi F, Bancroft JB, Watts JW & Burgess J (1973) J. Gen. Virol.20, 177–193.
Chupeau Y, Bourgin JP, Missonier C, Dorion N & Morel G (1974) C.R. Acad. Sci.278, 1565–1568.
Jarvis NP & Murakishi HH (1980) J. Gen. Virol.48, 365–376.
Nagata T & Takebe I (1970) Planta92, 301–308.
Takebe I & Otsuki Y (1969) Proc. Natl. Acad. Sci. U.S.A.64, 843–848.
Dawson JRO, Dickerson PE, King JM, Sakai F, Trim ARH & Watts JW (1978) Z. Naturforsch.33c, 548–551.
Pethig R (1979) Dielectric and Electronic Properties of Biological Materials, John Wiley & Sons, Chichester.
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Watts, J.W., King, J.M. A simple method for large-scale electrofusion and culture of plant protoplasts. Biosci Rep 4, 335–342 (1984). https://doi.org/10.1007/BF01140497
- Plant Protoplast