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Pregnancies following the frozen storage of expanding human blastocysts

  • Jacques Cohen
  • Roger F. Simons
  • Robert G. Edwards
  • Carole B. Fehilly
  • Simon B. Fishel
Article

Abstract

Human blastocysts were frozen in Earle's solution containing pyruvate and human serum, using glycerol as cryoprotectant, and stored in liquid nitrogen. Thawed blastocysts were replaced in 11 patients, which resulted in two pregnancies. One blastocyst giving a pregnancy was hatching when replaced. Three parameters appeared to be important for embryo survival and implantation: the interval between ovulation and replacement of the thawed blastocysts, satisfactory embryonic development before freezing, and the stage of blastulation when cooling began.

Key Words

cryostorage expanded human blastocysts 

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Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Jacques Cohen
    • 1
  • Roger F. Simons
  • Robert G. Edwards
    • 2
  • Carole B. Fehilly
  • Simon B. Fishel
    • 2
  1. 1.Bourn Hall ClinicCambridgeUK
  2. 2.Physiological LaboratoryUniversity of CambridgeCambridgeUK

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