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The use of tritiated cellulose in screening for cellulolytic microorganisms

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Phosphoric acid swollen cellulose has been reduced with3H-KBH4 and the resulting radioactive amorphous cellulose is broken down by a number of cellulolytic bacteria and fungi with accumulation of radioactive label in the culture filtrate. This is detected by scintillation counting. Non-cellulolytic microorganisms do not behave in a similar manner.

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Stewart, J.C., Stewart, C.S. & Heptinstall, J. The use of tritiated cellulose in screening for cellulolytic microorganisms. Biotechnol Lett 4, 459–464 (1982). https://doi.org/10.1007/BF01134595

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  • Cellulose
  • Phosphoric Acid
  • Scintillation Counting
  • Culture Filtrate
  • Amorphous Cellulose