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The hemizona assay (HZA): A predictor of human sperm fertilizing potential in in vitro fertilization (IVF) treatment

  • Daniel R. Franken
  • Sergio Oehninger
  • Lani Johnson Burkman
  • Charles C. Coddington
  • Thinus F. Kruger
  • Zev Rosenwaks
  • Anibal A. Acosta
  • Gary D. Hodgen
Special Contributions

Abstract

The hemizona assay (HZA) was developed to assess human sperm fertilizing potential. This blinded study investigated the relationship between sperm binding to the hemizona and in vitro fertilization (IVF) success (36 patients). Nonliving human oocytes were recovered from excised ovaries and stored. Each zona pellucida was cut into equal hemispheres by micromanipulation. For the HZA, one droplet exposed a hemizona to abnormal spermatozoa, while the control droplet contained the matching hemizona and spermatozoa from normal semen. After 4 hr, the number of tightly bound spermatozoa was counted. Binding to the hemizona was significantly higher for those having IVF success (mean of 36.1±7, versus 10.4±4 from the failure group;P<0.05). Fewer sperm from the failure group had a strictly normal morphology (3,2 versus 12.7%;P<0.05, Kruger method). Tight zona binding was significantly correlated with the percentage motile sperm, percentage normal morphology, and seminal sperm concentration. These results enhanced our confidence that the HZA is diagnostic for identification of patients at high risk of failing to achieve fertilization in vitro.

Key Words

zona pellucida human fertility sperm binding in vitro fertilization (IVF) 

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Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • Daniel R. Franken
    • 1
  • Sergio Oehninger
    • 4
  • Lani Johnson Burkman
    • 4
  • Charles C. Coddington
    • 2
  • Thinus F. Kruger
    • 3
  • Zev Rosenwaks
    • 4
  • Anibal A. Acosta
    • 4
  • Gary D. Hodgen
    • 4
  1. 1.Department of Obstetrics and Gynecology, Faculty of MedicineUniversity of the Organe Free StateBloemfonteinRepublic of South Africa
  2. 2.Department of Obstetrics and GynecologyPortsmouth Naval HospitalPortsmouth
  3. 3.Infertility ClinicTygerberg Hospital and University of StellenboschParow
  4. 4.The Jones I Institute for Reproductive Medicine, Department of Obstetrics and GynecologyEastern Virginia Medical SchoolNorfolk

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