Implementation of an in vitro fertilization program

  • Wulf H. Utian
  • James M. Goldfarb
  • Leon A. Sheean
Article

Abstract

The establishment of an in vitro fertilization program is described. Organization of the physical laboratory, media formulation, and preliminary mouse embryo culture are discussed. Parameters of patient eligibility, ovarian stimulation, and laporoscopy are also defined. At the conclusion of Phase I, 17 patients were induced; 10 went to laparoscopy, and at least one four-cell embryo was returned to 7, resulting in one continuing pregnancy.

Keywords

Public Health Developmental Biology Patient Eligibility Mouse Embryo Ovarian Stimulation 
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References

  1. 1.
    Biggers JD, Whitten WK, Whittingham DG: The culture of mouse embryos in vitro.In Methods in Mammalian Embryology, JC Daniel (ed). San Francisco, WH Freeman, 1971, pp 86–116Google Scholar
  2. 2.
    Trounson AD, Mohr LR, Wood C, Leeton JF: Effect of delayed insemination on in vitro fertilization, culture and transfer of human embryos. J Reprod Fertil 1982;64:285–294PubMedGoogle Scholar
  3. 3.
    Edwards RG, Prudy MM, Steptoe PC, Walters DE: The growth of human preimplantation embryos in vitro. Am J Obstet Gynecol 1981;141:408–416PubMedGoogle Scholar
  4. 4.
    Wentz AC, Torbit CA, Daniell JF, et al.: Combined screening laparoscopy and timed follicle aspiration for human in vitro fertilization. Fertil Steril 1983;39:270–276PubMedGoogle Scholar
  5. 5.
    Wortham JWE Jr, Veeck LL, Witmyer J, Jones HW: Vital initiation of pregnancy (VIP) using human menopausal gonadotropin and human chorionic gonadotropin ovulation induction: Phase I—1981. Fertil Steril 1983;39:785–792PubMedGoogle Scholar
  6. 6.
    Jones HW Jr, Acosta AA, Garcia JE, Sandow BA, Veeck L: On the transfer of conceptuses from oocytes fertilized in vitro. Fertil Steril 1983;39:241–243PubMedGoogle Scholar
  7. 7.
    Jones HW Jr, Jones GS, Andrews MC, et al: The program for in vitro fertilization at Norfolk. Fertil Steril 1982;38: 14–20PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1984

Authors and Affiliations

  • Wulf H. Utian
    • 1
  • James M. Goldfarb
    • 1
  • Leon A. Sheean
    • 1
  1. 1.Department of Obstetrics and Gynecology, The Mt. Sinai Medical Center of ClevelandCase Western Reserve University, University CircleCleveland

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