Further evaluation of the kinetic data for Fe3+ removal from isolated differic and monoferric transferrins by the tricatechol ligand 3,4-LICAMS has allowed full characterization of the four microscopic rate constants. A very small cooperativity exists between the two iron-binding sites with respect to their rates of iron release. The activation free energy profile for the system is presented.
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Kretchmar SA, Raymond KN (1986) Biphasic kinetics and temperature dependence of iron removal from transferrin by 3,4-LICAM. J Am Chem Soc 108:6212–6218
Aisen P (1989) Physical properties of the transferrins: Update 1984–1988. In: Loehr TM (ed) Iron carriers and iron proteins. VCH Press, Weinheim
Chasteen ND (1983) Transferrin: a perspective. In: Theil EC, Eichorn GL, Marzilli LG (eds) Advances in inorganic biochemistry, vol 5. Elsevier, New York, pp 201–233
Kretchmar SA, Teixeira M, Huynh B-H, Raymond KN (1988) Mössbauer studies of electrophoretically purified monoferric and diferric human transferrin. Biol Metals 1:26–32
Baldwin D (1980) The kinetics of iron release from human transferrin by EDTA. Effects of salts and detergents. Biochim Biophys Acta 623:183–198
Bertini I, Hirose J, Lichinat C, Messori L, Piccioli M, Scozzafava A (1988) Kinetic studies on metal removal from transferrins by pyrophosphate. Inorg Chem 27:2405–2409
Harris WR, Rezvani AB, Bali PK (1987) Removal of iron from transferrin by pyrophosphate and tripodal phosphonate ligands. Inorg Chem 26:2711–2716
Carrano CJ, Raymond KN (1979) Ferric ion sequestering agents. 2. Kinetics and mechanism of iron removal from transferrin by enterobactin and synthetic tricatechols. J Am Chem Soc 101:5401–5404
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Kretchmar, S.A., Raymond, K.N. Further evaluation of the biphasic kinetics of iron removal from transferrin by 3,4-LICAMS. Biol Metals 2, 65–68 (1989). https://doi.org/10.1007/BF01129202
- Biphasic kinetics
- Microscopic rate constants
- Catechol ligand