Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

Ferricrocin functions as the main intracellular iron-storage compound in mycelia ofNeurospora crassa

  • 92 Accesses

  • 25 Citations

Summary

Neurospora crassa produces several structurally distinct siderophores: coprogen, ferricrocin, ferrichrome C and some minor unknown compounds. Under conditions of iron starvation, desferricoprogen is the major extracellular siderophore whereas desferriferricrocin and desferriferrichrome C are predominantly found intracellularly. Mössbauer spectroscopic analyses revealed that coprogen-bound iron is rapidly released after uptake in mycelia of the wild-typeN.crassa 74A. The major intracellular target of iron distribution is desferriferricrocin. No ferritin-like iron pools could be detected. Ferricrocin functions as the main intracellular iron-storage peptide in mycelia ofN. crassa. After uptake of ferricrocin in both the wild-typeN. crassa 74A and the siderophore-free mutantN. crassa arg-5 ota aga, surprisingly little metabolization (11%) could be observed. Since ferricrocin is the main iron-storage compound in spores ofN. crassa, we suggest that ferricrocin is stored in mycelia for inclusion into conidiospores.

This is a preview of subscription content, log in to check access.

References

  1. Brambl S, Dunkle LD, Van Etten JL (1978) Nucleic acid and protein synthesis during fungal spore germination. In: Smith JE, Berry DR (eds) The filamentous fungi. Arnold, London, pp 94–118

  2. Brody S (1981) Genetic and biochemical studies on Neurospora conidia germination and formation. In: Turian G, Hohl HR (eds) The fungal spore: morphogenetic controls. Academic Press, New York, pp 605–626

  3. Chung TDY, Matzanke BF, Winkelmann G, Raymond KN (1986) Inhibitory effect of the partially resolved coordination isomers of chromic desferricoprogen on coprogen uptake inNeurospora crassa. J Bacteriol 165:283–287

  4. Dahlberg KR, Van Etten JL (1982) Physiology and biochemisty of fungal sporulation. Annu Rev Phytopathol 20:281–301

  5. Ernst JF, Winkelmann G (1977) Enzymatic release of iron from sideramines in fungi NADH:sideramine oxidoreductase inNeurospora crassa. Biochim Biophys Acta 500:27–41

  6. Horowitz NH, Charlang G, Horn G, Williams NP (1976) Isolation and identification of the conidial germination factor ofNeurospora crassa. J Bacteriol 127:135–140

  7. Huschka H, Naegeli HU, Leuenberger-Ryf H, Keller-Schierlein W, Winkelmann G (1985) Evidence for a common siderophore transport system but different siderophore receptors inNeurospora crassa. J Bacteriol 162:715–721

  8. Huschka H, Jalal MAF, Helm D van der, Winkelmann G (1986) Molecular recognition of siderophores in fungi: role of iron-surroundingN-acyl residues and the peptide backbone during membrane transport inNeurospora crassa. J Bacteriol 167:1020–1024

  9. Matzanke BF (1987) Mössbauer spectroscopy of microbial iron uptake and metabolism. In: Winkelmann G, Heim D van der, Neilands JB (eds) Iron transport in microbes, plants, and animals. Verlag Chemie, Weinheim, pp 251–284

  10. Matzanke B, Winkelmann G (1981) Siderophore iron transport followed by M6ssbauer spectroscopy. FEBS Lett 130:50–53

  11. Matzanke BF, Ecker DJ, Yang T-S, Huynh BH, Müller G, Raymond KN (1986a) Iron enterobactin uptake inEscherichia coli followed by Mössbauer spectroscopy. J Bacteriol 167:674–680

  12. Matzanke BF, Bill E, Winkelmann G, Trautwein AX (1986b) A57Fe Mössbauer study of iron assimilation inN. crassa mediated by siderophores. Hyperf Interact 29:1415–1418

  13. Matzanke BF, Bill E, Winkelmann G, Trautwein AX (1987a) Metabolization of57Fe-coprogen inN. crassa. An in vivo Mössbauer study. Eur J Biochem 162:643–650

  14. Matzanke BF, Bill E, Winkelmann G, Trautwein AX (1987b) A novel main component of microbial iron metabolism detected by in vivo Mössbauer spectroscopy. Rec Trav Chim Pays-Bas 106:258

  15. Matzanke BF, Bill E, Winkelmann G, Trautwein AX (1987c) Role of siderophores in iron storage compounds in spores ofN. crassa andA. ochraceus. J Bacteriol 169:5873–5876

  16. Matzanke BF, Müller-Matzanke G, Raymond KN (1988) Siderophore mediated iron transport; chemistry, biology and physical properties. In: Lever ABP, Gray HB (eds) Physical bio-inorganic chemistry series, vol IV. Addison-Wesley,Reading, MA (in press)

  17. Raymond KN, Miller GI, Matzanke BF (1984) Complexation of iron by siderophores. A review of their solution and structural chemistry and biological function. Top Curr Chem 123:49–102

  18. Tufano TP, Raymond KN (1981) Coordination chemistry of microbial iron compounds. 21. Kinetics and mechanism of iron exchange in hydroxamate siderophore complexes J Am Chem Soc 103:6617–6624

  19. Van Etten JL, Dahlberg KR, Russo GM (1981) Nucleic acids. In: Turian G, Hohl HR (eds) The fungal spore: morphogenetic controls. Academic Press, New York, pp 277–302

  20. Wong GB, Kappel MJ, Raymond KN, Matzanke B, Winkelmann G (1983) Coordination chemistry of microbial iron transport compounds. 24. Characterization of coprogen and ferricrocin, two ferric hydroxamate siderophores. J Am Chem Soc 105:810–815

Download references

Author information

Correspondence to Berthold F. Matzanke.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Matzanke, B.F., Bill, E., Trautwein, A.X. et al. Ferricrocin functions as the main intracellular iron-storage compound in mycelia ofNeurospora crassa . Biol Metals 1, 18–25 (1988). https://doi.org/10.1007/BF01128013

Download citation

Key words

  • Siderophores
  • Ferricrocin
  • Iron storage
  • Sporulation
  • In vivo Mössbauer spectroscopy