Biology of Metals

, Volume 1, Issue 1, pp 9–17 | Cite as

High-performance liquid chromatography of siderophores from fungi

  • Silvia Konetschny-Rapp
  • Hans-Georg Huschka
  • Günther Winkelmanne
  • Günther Jung
Original Articles

Summary

A reversed-phase HPLC separation of iron(III) chelates of 16 representative fungal siderophores including ferrichromes, coprogens and triacetylfusarinine C was established in order to investigate siderophore production of fungi. For comparison purposes, the widely used bacterial siderophore ferrioxamine B was included. Culture filtrates of the fungiPenicillium resticulosum, Fusarium dimerum, Aspergillus fumigatus andNeurospora crassa were quantitatively analyzed for the presence of known and unknown siderophores after growth in low-iron culture media and adsorption on XAD-2 columns using this HPLC separation system. Photodiode array detection allowed the distinction between siderophores and non-siderophores. According to their ultraviolet/visible spectra, a further classification of the siderophores into four types due to the number of anhydromevalonic acid residues per molecule (0–3) was possible.

Key words

Siderophores HPLC separation Identification Fungi 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Silvia Konetschny-Rapp
    • 1
  • Hans-Georg Huschka
    • 2
  • Günther Winkelmanne
    • 2
  • Günther Jung
    • 1
  1. 1.Mikrobiologie 1Institut für Organische ChemieTübingenGermany
  2. 2.Mikrobiologie 1Institut für Biologie ITübingenGermany

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