Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

Partial purification of a boar sperm membrane protein inducing sperm agglutinating antibody

  • 8 Accesses

Abstract

For the development of immunological contraception, attention is being concentrated on the possibility of using a sperm membrane antigen. Boar sperm membrane was extracted with triton-X 100 and fractionated by Sephadex G-150 column chromatography. The glycosylated and nonglycosylated portions of protein peaks from the gel filtration were obtained by fractionating on concanavalin A-Sepharose and eluting the bound protein with 0.3 M methyl mannoside. A glycosylated fraction was found to induce sperm agglutinating antibodies in rabbit. The partially purified protein has a molecular weight of 30 kilodaltons, as determined by sodium dodecyl polyaccyrlamide gel electrophoresis. Further work is planned on the histochemical determination of the origin of this protein and species cross-activity of the antibody.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Naz, R. K., Saxe, J. M. and Merger, A. C. (1983)Biol. Reprod. 28:249.

  2. 2.

    O'Rand, M. G. (1981)Biol. Reprod. 25:611.

  3. 3.

    Schwartz, M. A. and Koehler, J. K. (1979)Biol. Reprod. 21:1295.

  4. 4.

    Gabel, C. A., Eddy E. M. and Shapiro, B. M. (1979)J. Cell Biol. 82:742.

  5. 5.

    Tung, K. S. K. (1975)Clin. Exp. Immunol. 20:93.

  6. 6.

    Herr, J. C. and Eddy, E. M. (1980)Biol. Reprod. 22:1263.

  7. 7.

    Myles, D. C., Primakoff, P. and Bellve, A. R. (1981)Cell 23:433.

  8. 8.

    Wolf, D. P., Sokoloski, J. E., Dandekar, P. and Bechtol, K. B. (1983)Biol. Reprod. 29:713.

  9. 9.

    Tung, K. S. K., Okada, A. and Yanagimachi, R. (1980)Biol. Reprod. 23:877.

  10. 10.

    O'Rand, M. G. and Porter, J. R. (1979)J. Immunol. 122:1248.

  11. 11.

    Hagopian, A., Jackson, J. J., Carlo, D. J., Limjuco, G. A. and Eylar, E. H. (1975)J. Immunol. 115:1731.

  12. 12.

    Wang, L. F., Miao, S. Y., Cao, S. L., Wu, B. Y. and Koide, S. S. (1986)Arch. Androl. 16:55.

  13. 13.

    Gillis, G., Peterson, R. N., Russell, L., Hook, L. and Freund, M. (1978)Prep. Biochem. 8:363.

  14. 14.

    Peterson, R. N., Russell, L., Bundman, D. and Freund, M. (1979)Biol. Reprod. 21:583.

  15. 15.

    Peterson, R. N., Russell, L., Bundman, D. and Freund, M. (1980)Science 207: 73.

  16. 16.

    Peterson, R. N., Russell, L., Spaulding, G., Bundman, G., Buchanan, J. and Freund, M. (1981a)J. Androl. 2:300.

  17. 17.

    Peterson, R. N., Russell, L., Bundman, D., Conway, M. and Freund, M. (1981b)Dev. Biol. 84:144.

  18. 18.

    Helenius, A. and Simon, K. (1975)Biochem Biophys. Acta 415:29.

  19. 19.

    Tanford, C. and Reynolds, J. (1976)Biochem. Biophys. Acta 457:133.

  20. 20.

    Klint, M., Sege, K., Curman, B., Ploan, L. and Peterson, P. A. (1985)Gamete Res. 11:335.

  21. 21.

    Lowry, G. H., Rosebrough, N. J., Far, A. L. and Randall, R. J. (1951)J. Biol. Chem. 193:265.

  22. 22.

    Glenys, A., Bloomfield, M., Faith, R. and Pierce, J. C. (1978)Biochim. Biophys. Acta 553:371.

  23. 23.

    Vaitukaitis, J., Robbins, J. B., Nieschlag, E. and Ross, C. T. (1971)J. Clin Endocr. 33:988.

  24. 24.

    Friberg, J. (1974)Acta Obstet Gynaecol Scand. 36:21.

  25. 25.

    Laemli, U. K. (1970)Nature 227:680.

  26. 26.

    Haas, G. C., Jr. Cines, D. B. and Schreiber, A. D. (1980)N. Engl. Med. 303:722.

  27. 27.

    Isojima, S., Tsuchiya, K., Koyama, K., Tonaka, C., Naka, O. and Adachi, H. (1972)Am J Obstet Gynecol,112:199.

  28. 28.

    Metz, C. B. (1973)Fed Prod. 32:2037.

  29. 29.

    Isahakia, M. and Alexander, N. J. (1984)Biol. Reprod. 30:1015.

  30. 30.

    Fawcett, D. M., Anderson, W. and Phillips, D. M. (1977)Dev. Biol. 26:220.

  31. 31.

    Flechon, J. E. (1979)Gamete Res. 2, 43.

  32. 32.

    Paulsen and Hjort, T. (1981)J. Clin. Lab. Immunol. 6:69.

  33. 33.

    Goodman, S. A. and Young, L. G. (1981)J. Reprod. Immunol. 3, 99.

  34. 34.

    Yan, Y. C., Wang, L. F. and Koide, S. S. (1987)Arch. Androl. 18:245.

  35. 35.

    Li, T. S. and Shulmain, S. (1971)Int. J. Fertil. 16:87.

  36. 36.

    Mettler, L. and Gradl, I. (1975)Res Exp Med 165:169.

Download references

Author information

Correspondence to Oyewole Adeyemo.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Adeyemo, O., Okegbile, E.O. & Olorunsogo, O.O. Partial purification of a boar sperm membrane protein inducing sperm agglutinating antibody. Biosci Rep 10, 131–139 (1990). https://doi.org/10.1007/BF01116572

Download citation

Key Words

  • membrance protein
  • boar sperm
  • sperm agglutinating antibody