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Partial purification of a boar sperm membrane protein inducing sperm agglutinating antibody

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For the development of immunological contraception, attention is being concentrated on the possibility of using a sperm membrane antigen. Boar sperm membrane was extracted with triton-X 100 and fractionated by Sephadex G-150 column chromatography. The glycosylated and nonglycosylated portions of protein peaks from the gel filtration were obtained by fractionating on concanavalin A-Sepharose and eluting the bound protein with 0.3 M methyl mannoside. A glycosylated fraction was found to induce sperm agglutinating antibodies in rabbit. The partially purified protein has a molecular weight of 30 kilodaltons, as determined by sodium dodecyl polyaccyrlamide gel electrophoresis. Further work is planned on the histochemical determination of the origin of this protein and species cross-activity of the antibody.

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Correspondence to Oyewole Adeyemo.

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Adeyemo, O., Okegbile, E.O. & Olorunsogo, O.O. Partial purification of a boar sperm membrane protein inducing sperm agglutinating antibody. Biosci Rep 10, 131–139 (1990).

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Key Words

  • membrance protein
  • boar sperm
  • sperm agglutinating antibody