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Bioscience Reports

, Volume 5, Issue 5, pp 383–391 | Cite as

The amino acid sequence of human sperm protamine P1

  • D. J. McKay
  • B. S. Renaux
  • G. H. Dixon
Article

Abstract

Human sperm protamines have been extracted from spermatozoa pooled from several donors, converted to their S-pyridylethylated derivatives and resolved into two major components, P1 and PI, by Bio-Rex 70 chromatography. Protamine P1 was further purified by Bio-Gel P-10 chromatography and sequenced directly on a gas phase protein sequencer for 43 residues. To complete the sequence, P1 was cleaved at methionine 36 and the C-terminal tetradecapeptide was purified by h.p.i.c , and sequenced completely. The 50 residue sequence is: 10 20 30 40 ARYRC CRSQS RSRYY RQRQR SRRRR RRSCQ TRRRA MRCCR 50 PRYRP RCRRH. This sequence has a calculated molecular weight of 6674 and is homologous with four other published mammalian protamine sequences.

Keywords

Molecular Weight Amino Acid Sequence Methionine Protein Sequencer Protamine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© The Nobel Foundation 1985

Authors and Affiliations

  • D. J. McKay
    • 1
  • B. S. Renaux
    • 1
  • G. H. Dixon
    • 1
  1. 1.Department of Medical Biochemistry, Faculty of MedicineUniversity of Calgary, Health Sciences CentreCalgaryCanada

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