Bioscience Reports

, Volume 5, Issue 5, pp 383–391 | Cite as

The amino acid sequence of human sperm protamine P1

  • D. J. McKay
  • B. S. Renaux
  • G. H. Dixon


Human sperm protamines have been extracted from spermatozoa pooled from several donors, converted to their S-pyridylethylated derivatives and resolved into two major components, P1 and PI, by Bio-Rex 70 chromatography. Protamine P1 was further purified by Bio-Gel P-10 chromatography and sequenced directly on a gas phase protein sequencer for 43 residues. To complete the sequence, P1 was cleaved at methionine 36 and the C-terminal tetradecapeptide was purified by h.p.i.c , and sequenced completely. The 50 residue sequence is: 10 20 30 40 ARYRC CRSQS RSRYY RQRQR SRRRR RRSCQ TRRRA MRCCR 50 PRYRP RCRRH. This sequence has a calculated molecular weight of 6674 and is homologous with four other published mammalian protamine sequences.


Molecular Weight Amino Acid Sequence Methionine Protein Sequencer Protamine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Balhorn R, Gledhill BL & Wyrobek AJ (1977) Biochem.16, 4074–4080.Google Scholar
  2. Bellvé AR, Anderson E & Hanley-Bowdoin L (1975) Dev. Biol.47, 349–365.Google Scholar
  3. Bloch DP (1969) Genetics61, Suppl. 1, 93–111.Google Scholar
  4. Coelingh JP, Pozijn TH & Monfoort CH (1969) Biochim. Biophys. Acta188, 353–356.Google Scholar
  5. Coelingh JP, Monfoort CH, Rozijn TH, Leuven JAG, Schiphof R, Steyn-Parve EP, Braunitzer G, Schrank B & Ruhfus A (1972) Biochim. Biophys. Acta285, 1–14.Google Scholar
  6. Dayhoff MO, Eck RV & Park CM (1972) In: Atlas of Protein Sequence and Structure (Dayhoff MO, ed), Washington, National Biomedical Research Foudation.Google Scholar
  7. Dixon GH, Aiken JM, Jankowski JM, McKenzie DI, Moir R & States JC (1985) Proc. Advanced Study Institute, Sitges, Spain, in press.Google Scholar
  8. Gaastra W, Hofstra JL & Kolk AHJ (1978) Biochem. Genetics16, 525–529.Google Scholar
  9. IUPAC — ‘A One Letter Notation for Amino Acid Sequeces’ (1968) Eur. J. Biochem5, 151–153. A=Alanine, C=Cysteine, F=Phenylalanine, G=Glycine, H=Histidine, I=Isoleucine, K=Lysine, L=Leucine, M=Methionine, P=Proline, Q=Glutamine, R=Arginine, S=Serine, T=Threonine, V=Valine, and Y=Tyrosine.Google Scholar
  10. Kleene KC, Distel RJ & Hecht NB (1985) Biochemistry24, 719–722.Google Scholar
  11. Kolk AHJ & Samuel T (1975) Biochim. Biophys. Acta393, 307–319.Google Scholar
  12. Monfoort CH, Schiphof R, Rozijn TH & Steyn-Parve EP (1973) Biochim. Biophys. Acta322, 173–177.Google Scholar
  13. Pongsawasdi P & Svasti J (1976) Biochim. Biophys. Acta434, 462–473.Google Scholar
  14. Sautiere P, Belaiche D, Martinage A & Loir M (1984) Eur. J. Biochem.144, 121–125.Google Scholar
  15. Svasti J & Talupphet N (1979) Biochim. Biophys. Acta577, 221–225.Google Scholar
  16. Tobita T, Tsutsumi H, Kato A, Suzuki H, Nomoto M, Nakano M & Ando T (1983) Biochim. Biophys. Acta744, 141–146.Google Scholar

Copyright information

© The Nobel Foundation 1985

Authors and Affiliations

  • D. J. McKay
    • 1
  • B. S. Renaux
    • 1
  • G. H. Dixon
    • 1
  1. 1.Department of Medical Biochemistry, Faculty of MedicineUniversity of Calgary, Health Sciences CentreCalgaryCanada

Personalised recommendations