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A radioimmunocytological quantitative method for the rapid detection of ras oncogene p21 protein in mammalian cells

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Abstract

In order to provide a sensitive and quantitative detection method of ras p21 at the cytological level, the monoclonal antibody Y 13 259 and iodinated protein A were used to locate theras protein in various mammalian cell lines. The subsequent autoradiograph can be analysed by a computer-assisted system which showed in these reported experiments that the relative levels of p21 detected in these cells corresponded to results obtained earlier using conventional biochemical methods.

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Spandidos, D.A., Dimitrov, T. & Kam, E.Y. A radioimmunocytological quantitative method for the rapid detection of ras oncogene p21 protein in mammalian cells. Biosci Rep 6, 375–379 (1986). https://doi.org/10.1007/BF01116424

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Key Words

  • Radioimmuno assay
  • ras oncogene
  • oncogene
  • p21 protein