Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

Assembly of DNA onto the histone octamer facilitates the B-to-Z transition

  • 10 Accesses


Nucleosomal core particles containing the right- and left-handed conformations of DNA were examined for their ability to support the B→Z or Z→B transition. Nucleosomes were assembled onto the B- and Z-conformations of poly[d(Gm5C)] and the B-conformation of poly[d(GC)] as previously described (1). Absorbance and circular dichroic spectroscopy indicated that the DNA on all three core particle populations could undergo the conformational B↔Z transition. Further, the right- to left-handed transition for both poly[d(Gm5C)] and poly[d(GC)] appeared to be facilitated by the DNAs association with the histone octamer. The DNA remained associated with the protein core subsequent to the transition, and electron microscopy and sedimentation velocity analysis indicated that there were no gross changes in nucleosomal structure. However, a change in the sedimentation value of the poly[d(Gm5C)] core particles was detected when the conformation of the DNA was altered from B to Z, resulting in a lower S20,w value for the Z-form particles than for the corresponding B-form particles.

This is a preview of subscription content, log in to check access.


  1. 1.

    Miller, F. D., Dixon, G. H., Rattner, J. B., and van de Sande, J. H. (1985).Biochem. 24:102–109.

  2. 2.

    Miller, F. D., Rattner, J. B., and van de Sande, J. H. (1982)Cold Spring Harbor Symp. Quant. Biol. 47:571–575.

  3. 3.

    Klevan, L., and Schumaker, V. N. (1982).Nuc. Acids Res. 10:6809–6817.

  4. 4.

    Seeman, N. C., Rosenberg, J. M., and Rich, A. (1976).Proc. Natl. Acad. Sci. USA 73: 804–808.

  5. 5.

    Russell, W. C., Precious, B., Martin, S. R., and Bayley, P. M. (1983).The EMBO J. 2:1647–1653.

  6. 6.

    Gill, J. E., Mazirmas, J. A., and Bishop, C. C. Jr. (1974).Biochim. Biophys. Acta 335: 330–348.

  7. 7.

    van der Westhuizen, D. R., and von Holt, C. (1971)FEBS Letts. 14:333–337.

  8. 8.

    van de Sande, J. H., McIntosh, L. P., and Jovin, T. M. (1982).The EMBO J. 1: 777–782.

  9. 9.

    van de Sande, J. H., and Jovin, T. M. (1982).The EMBO J. 1:115–120.

  10. 10.

    Nickol, J., Behe, M., and Felsenfeld, G. (1982).Proc. Natl. Acad. Sci. USA 79: 1771–1775.

  11. 11.

    Ichimura, S., Mita, K., and Zama, M. (1982).Biochem. 21:5329–5334.

  12. 12.

    Rill, R. L., and Oosterhof, D. K. (1982).J. Biol. Chem. 257:14875–14880.

  13. 13.

    McGhee, J. D., and Felsenfeld, G. (1979).Proc. Natl. Acad Sci. USA 76:2133–2137.

  14. 14.

    Cantor, C. (1976).Proc. Natl. Acad. Sci. USA 73:3391–3393.

  15. 15.

    Nordheim, A., and Rich, A. (1983a).Nature (London) 303:674–679.

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Miller, F.D., Rattner, J.B. & van de Sande, J.H. Assembly of DNA onto the histone octamer facilitates the B-to-Z transition. Biosci Rep 6, 467–476 (1986). https://doi.org/10.1007/BF01116138

Download citation

Key Words

  • B-Z DNA
  • nucleosome
  • histone