Planta

, Volume 196, Issue 4, pp 706–711 | Cite as

An auxin-resistant mutant ofNicotiana plumbaginifolia Viv. is impaired in 1-naphthaleneacetic acid-induced hyperpolarization of hypocotyl cell membranes in intact seedlings

  • Petra Stirnberg
  • Patrick J. King
  • Hélène Barbier-Brygoo
Article
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Accepted:

Abstract

The electrical response to the synthetic auxin 1-naphthaleneacetic acid (1-NAA) ofNicotiana plumbaginifolia wild type and the monogenic, dominant auxinresistant mutant R25 was studied. Membrane potentials were continuously recorded in hypocotyl cells of light-grown, intact seedlings, and the time course of the response to 1-NAA addition was followed. Wild-type cells responded to ⩾ 10−5 M 1-NAA with a delayed, transient hyperpolarization. The R25 cells hyperpolarized significantly only in response to 1-NAA at 10−3 M, and with maximal amplitudes lower than those recorded with the wild type. In contrast, the two genotypes reacted similarly in terms of kinetics and amplitude to 10−5 M fusicoccin, which rapidly and strongly hyperpolarized the cells, and to 10−3 M benzoic acid, which induced rapid and weak hyperpolarization. The resting membrane potentials of the wild type and R25 were also not significantly different. Unlike wild-type hypocotyls, those of R25 ceased elongating before the time chosen for the electrophysiological measurements, but control experiments performed at a time when the elongation of both genotypes had terminated indicated that the difference in electrical response to auxin is independent of hypocotyl growth. The inefficiency of 1-NAA in inducing hyperpolarization of R25 hypocotyl cells suggests a defect at an early step in auxin action.

Key words

Auxin response mutant Fusicoccin Hypocotyl Membrane potential Nicotiana weak acid 

Abbreviation

1-NAA

1-naphthaleneacetic acid

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Petra Stirnberg
    • 1
  • Patrick J. King
    • 1
  • Hélène Barbier-Brygoo
    • 2
  1. 1.Friedrich Miescher-InstitutBaselSwitzerland
  2. 2.Institut des Sciences Végétales, C.N.R.S., Bât. 22Gif-sur-Yvette CedexFrance

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