Mycopathologia

, Volume 124, Issue 3, pp 189–194

Immunosuppressive effects of aflatoxin in growing rats

  • S. Raisuddin
  • K. P. Singh
  • S. I. A. Zaidi
  • B. N. Paul
  • P. K. Ray
Mycotoxicoses And Mycotoxins

DOI: 10.1007/BF01103737

Cite this article as:
Raisuddin, S., Singh, K.P., Zaidi, S.I.A. et al. Mycopathologia (1993) 124: 189. doi:10.1007/BF01103737

Abstract

The immunosuppressive potential of aflatoxin B1 (AFB1), the carcinogenic metabolite ofAspergillus flavus, was evaluated in growing rats. The weanling rats were subchronically exposed to 60, 300 and 600 µg AFB1/kg body weight for four weeks on alternate days by oral feeding. Various parameters of cell mediated immunity (CMI) and humoral immunity were assessed in control and treated animals. CMI was evaluated by measuring delayed type of hypersensitivity (DTH) response and humoral by plaque forming cell (PFC) assay. The lymphoproliferative response assay for T- and B-cells was also performed. It was observed that AFB1 selectively suppressed cell mediated immunity in growing rats. AFB1 suppressed CMI at the 300 and 600 µg dose levels only as measured by DTH response assay. It is concluded that continuous low level exposure of aflatoxin to growing host may enhance its susceptibility to infection and tumorigenesis.

Key words

Aflatoxin B1 Cellular immunity Immunosuppression Weanling rats 

Abbreviations

AF

Aflatoxin

AFB1

Aflatoxin B1

CMI

Cell mediated immunity

CPM

Counts per minute

DTH

Delayed type of hypersensitivity

GST

Glutathione-S-transferase

LPS

Lipopolysaccharide

PFC

Plaque forming cell

PHA

Phytohemagglutinin

SRBC

Sheep red blood cells

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • S. Raisuddin
    • 1
  • K. P. Singh
    • 2
  • S. I. A. Zaidi
    • 3
  • B. N. Paul
    • 2
  • P. K. Ray
    • 1
  1. 1.Environmental Sciences SectionBose InstituteCalcuttaIndia
  2. 2.Industrial Toxicology Research Centre, Mahatma Gandhi MargLucknowIndia
  3. 3.Department of DermatologyVeterans Administration Medical CentreClevelandUSA

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