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Preharvest aflatoxin contamination of maize inoculated withAspergillus flavus andFusarium moniliforme


A two-year factorial experiment was utilized to test plants field-inoculated singly and in combination withAspergillus flavus andFusarium moniliforme. Pinbar inoculations were made through the husks with conidial suspensions, and 10-ear maize samples were harvested at 60 days post-silking for aflatoxin determinations. When ears were inoculated with both fungi simultaneously,F. moniliforme reduced aflatoxin formation byA. flavus isolate NRRL 3357 by approximately two-thirds.F. moniliforme had no significant effect on naturally occurring aflatoxin contamination byA. flavus. This may be due to the timing of infection by both fungi in the field. In nature,A. flavus andF. moniliforme respond differently to the environment, offering one explanation of whyF. moniliforme did not measurably affect the other fungus.

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  1. 1.

    Widstrom NW. Aflatoxin in developing maize: Interaction among involved biota and pertinent econiche factors. In: Bhatnager D, Lillehoj EB, Arora DK (eds), Handbook of applied mycology, Vol. 5. New York: Marcel Dekker, 1992; 23–57.

  2. 2.

    Widstrom NW, McMillian WW, Beaver RW, Wilson DM. Weather-associated changes in aflatoxin contamination of preharvest maize. J Prod Agric 1990; 3: 196–99.

  3. 3.

    McMillian WW, Widstrom NW, Beaver RW, Wilson DM. Aflatoxin in Georgia: Factors associated with its formation in corn. Iowa Agric and Home Econ Exp Stn Res Bull 1991; 599: 329–34.

  4. 4.

    Wicklow DT, Hesseltine CW, Shotwell OL, Adams GL. Interference competition and aflatoxin levels in corn. Phytopathology 1980; 70: 761–64.

  5. 5.

    Horn BW, Wicklow DT. Factors influencing the inhibition of aflatoxin production in corn byAspergillus niger. Can J Microbiol 1983; 29: 1087–91.

  6. 6.

    Cuero R, Smith JE, Lacey J. Mycotoxin formation byAspergillus flavus andFusarium graminearum in irradiated maize grains in the presence of other fungi. J Food Prot 1988; 51: 452–56

  7. 7.

    Devi GR, Polasa H. Interference in toxin production among toxigenicAspergillus species. J Stor Prod Res 1987; 23: 149–50.

  8. 8.

    Choudhary AK. Influence of microbial co-inhabitants on aflatoxin synthesis of Aspergillus flavus on maize kernels. Lett Appl Microbiol 1992; 14: 143–47.

  9. 9.

    Wicklow DT, Horn BW, Shotwell OL. Aflatoxin formation in preharvest maize ears coinoculated withAspergillus flavus andAspergillus niger. Mycologia 1987; 79: 679–82.

  10. 10.

    Wicklow DT, Horn BW, Shotwell OL, Hesseltine CW, Caldwell, RW. Fungal interference withAspergillus flavus infection and aflatoxin contamination of maize grown in a controlled environment. Phytopathology 1988; 78: 68–74.

  11. 11.

    Ehrlich K, Ciegler A, Klich M, Lee L. Fungal competition and mycotoxin production on corn. Experientia 1985; 41: 691–693.

  12. 12.

    Lillehoj EB, Kwolek WJ, Guthrie WD, Barry D, McMillian WW, Widstrom NW. Aflatoxin accumulation in preharvest maize kernels: interaction of three fungal species, European corn borer and two hybrids. Plant Soil 1982; 65: 95–102.

  13. 13.

    Brown RL, Cotty PJ, Cleveland TE. Reduction in aflatoxin content of maize by atoxigenic strains ofAspergillus flavus. J Food Prot 1991; 54: 623–26.

  14. 14.

    Zummo N, Scott GE. Interaction ofFusarium moniliforme andAspergillus flavus on kernel infection and aflatoxin contamination in maize ears. Plant Dis 1992; 76: 771–73.

  15. 15.

    Zummo N, Scott GE. Relative aggressiveness ofAspergillus flavus andA. parasiticus on maize in Mississippi. Plant Dis 1990; 74: 978–81.

  16. 16.

    Zummo N. Concurrent infections of individual corn kernels with white and green isolates ofAspergillus flavus. Plant Dis 1991; 75: 910–13.

  17. 17.

    Wilson DM, McMillian WW, Widstrom NW. Use ofAspergillus flavus andA. parasiticus color mutants to study aflatoxin contamination. In: Llewellan G, O'Rear C, Barry S (eds), Biodeterioration, Vol. 6. London: International Biodeterioration Society, 1986: 284–88.

  18. 18.

    Choudhary AK, Sinha KK. Competition between a toxigenic Aspergillus flavus strain and other fungi on stored maize kernels. J Stored Prod Res 1993; 29: 75–80.

  19. 19.

    Thean JF, Lorenz DR, Wilson DM, Rodgers K, Gueldner RC. Extraction, cleanup, and quantitative determination of aflatoxin in corn. J Assoc Off Anal Chem 1980; 63: 631–33.

  20. 20.

    Trucksess MW, Stack ME, Nesheim S, Page SW, Albert RH, Hansen TJ, Donahue KF. Immunoaffinity column coupled with solution fluorometry or liquid chromatography postcolumn derivitization for determination of aflatoxins in corn, peanuts and peanut butter: collaborative study. J Assoc Off Anal Chem 1991; 74: 81–88.

  21. 21.

    Cochran WG, Cox GM. Experimental Designs, 2nd Ed. New York: John Wiley, 1957.

  22. 22.

    Steel RGD, Torrie JH. 1960. Principles and Procedures of Statistics. New York: McGraw-Hill, 1960.

  23. 23.

    Waller RA, Duncan DB. A Bayes rule for the symmetric multiple comparison problem. J Am Stat Assoc 1969; 64: 1484–99.

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Correspondence to N. W. Widstrom.

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Widstrom, N.W., McMillian, W.W., Wilson, D.M. et al. Preharvest aflatoxin contamination of maize inoculated withAspergillus flavus andFusarium moniliforme . Mycopathologia 128, 119–123 (1994). https://doi.org/10.1007/BF01103019

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Key words

  • Competition effects
  • Corn
  • Inoculation
  • Mycotoxin
  • Zea mays L.