The effects of μ, δ, and κ receptor-agonists on forskolin stimulated cyclic adenosine-3′, 5′-monophosphate (cAMP) formation were examined in astroglial enriched primary cultures from the cerebral cortex of newborn rats. Intracellular cAMP accumulation was quantified by radioimmunoassay. Morphine was used as a μ-receptor agonist, D-Ala-D-Leu-Enkephalin (DADLE) as a δ-receptor agonist and dynorphine 1–13 (Dyn) as a κ-receptor agonist. Basal cAMP levels were unaffected by either the opiate agonists or the antagonists used. In the presence of the cAMP stimulator forskolin, morphine had no significant effect on the cytoplasmic cAMP levels. DADLE caused a dose related inhibition of the forskolin stimulated cAMP accumulation. The effects of this δ receptor stimulation was blocked with the selective antagonist ICI 174.864. In the presence of Dyn, the forskolin stimulated cAMP accumulation was inhibited in a dose related manner. This κ receptor stimulation was blocked with the selective antagonist MR 2266. Co-administration of DADLE and Dyn resulted in a non additive inhibition of the forskolin stimulated accumulation of cAMP. These findings indicate that astroglial enriched cultures from the cerebral cortex of rats express δ and κ-receptors co-localized ont he same population of cells, and that these receptors are inhibitory coupled to adenylate cyclase.
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Attali, B., Saya, D., and Vogel, Z. 1989. κ-Opiate agonists inhibit adenylate cyclase and pruduce heterologous desensitization in rat spinal cord. J. Neurochem. 52:360–369.
Barr, G. A., Paredes, W., Erickson, K. L., and Zukin, R. S. 1986. κ-opioid receptor-mediated analgesia in the developing rat. Dev. Brain Res. 29:145–152.
Bunn, S. J., Hanley, M. R., and Wilkin, G. P. 1985. Evidence for a kappa-opioid receptor on pituitary astrocytes: an autoradiographic study. Neurosci. Lett. 55:317–323.
Chneiweiss, H., Glowinski, J., and Premont, J. 1988. Mu and delta opiate receptors coupled negatively to adenylate cyclase on embryonic neurons from the mouse striatum in primary cultures. J. Neurosci. 9:3376–3382.
Cholewinski, A. J., Hanley, M. R., and Wilkin, G. P. 1988. A Phosphoinositide-linked peptide response in astrocytes: Evidence for regional heterogeneity. Neurochem. Res. 13:389–394.
Cholewinski, A. J., and Wilkin, G. P. 1988. Astrocytes from forebrain, cerebellum, and spinal cord differ in their responses to vasoactive intestinal peptide. J. Neurochem. 51:1626–1633.
Cummins, C. J., Lust, W. D., and Passonneau, J. V. 1983. Regulation of glycogen metabolism in primary and transformed astrocytes in vitro. J. Neurochem. 40:128–136.
Hansson, E. 1988. Astroglia from defined brain regions as studied with primary cultures. Prog. Neurobiol. 30:369–397.
Hansson, E. 1986. Primary astroglial cultures. A biochemical and functional evaluation. Neurochem. Res. 11:759–767.
Hansson, E., Eriksson, P., and Nilsson, M. 1985. Amino acid and monoamine transport in primary astroglial cultures from defined brain regions. Neurochem. Res. 10:1335–1341.
Hansson, E., and Rönnbäck, L. 1989. Regulation of glutamate and GABA transport by adrenoceptors in primary astroglial cell cultures. Life Sci. 44:27–34.
Hansson, E., and Rönnbäck, L. 1988. Interaction between catecholamines and vasoactive intestinal peptide in cultured astrocytes. Neuropharmacology. 27:295–300.
Hansson, E., Rönnbäck, L., Persson, L. I., Lowenthal, A., Noppe, M., Alling, C., and Karlsson, B. 1984. Cellular composition of primary cultures from cerebral cortex, striatum, hippocampus, brainstem and cerebellum. Brain Res. 300:9–18.
Hansson, E., Simonsson, P., and Alling, C. 1987. 5-hydroxytryptamine stimulates the formation of inositol phosphate in astrocytes from different regions of the brain. Neuropharmacology 26:1377–1382.
Law, P.-Y., Hom, D. S., and Loh, H. H. 1985. Multiple affinity states of opiate receptor in Neuroblastoma x glioma NG108-15 hybrid cells. J. Biol. Chem. 260:3561–3569.
Law, P. Y., Wu, J., Koehler, J. E., and Loh, H. H. 1981. Demonstration and characterization of opiate inhibition of the striatal adenylate cyclase. J. Neurochem. 36:1834–1846.
Lightman, S. L., Nincovic, M., Hunt, S. P., and Iversen, L. L. 1983. Evidence for opiate receptors on pituicytes. Nature. 305:235–237.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.
Maderspach, K., and Solomonia, R. 1988. Glial and neuronal opioid receptors: apparent positive cooperativity observed in intact cultured cells. Brain Res. 441:41–47.
Mansour, A., Lewis, M. E., Khachaturian, H., Akil, H., and Watson, S. J. 1986. Pharmacological and anatomical evidence of selective μ, δ, and κ opioid receptor binding in rat brain. Brain Res. 399:69–79.
McCarthy, K. D., Prime, J., Harmon, T., and Pollenz, R. 1985. Receptor-mediated phosphorylation of astroglial intermediate filament proteins in cultured astroglia. J. Neurochem. 44:723–730.
McDowell, J., and Kitchen, I. 1986. Ontogenesis of δ-opioid receptors in rat brain using [3H][D-pen2, D-pen5]enkephalin as a binding ligand. Eur. J. Pharmacol. 128:287–289.
McDowell, J., and Kitchen, I. 1987. Development of opiod systems: peptides, receptors and pharmacology. Brain Res. Rev. 12:397–421.
McLean, S., Rothman, R. B., and Herkenham, M. 1986. Autoradiographic localization of μ- and δ-opiate receptors in the forebrain of the rat. Brain Res. 378:49–60.
Murphy, S., and Pearce, B. 1987. Functional receptors for neurotransmitters on astroglial cells. Neuroscience. 22:381–394.
Pearce, B., Cambray-Deakin, M., and Murphy, S. 1985. Astrocyte opioid receptors: Activation modifies the noradrenaline-evoked increase in 2-[14C]deoxyglucose incorporation into glycogen. Neurosci. Lett. 55:157–160.
Schoffelmeer, A. N. M., Hogenboom, F., and Mulder, A. H. 1987. Inhibition of dopamine-sensitive adenylate cyclase by opioids: possible involvement of physically associated μ-and δ-opioid receptors. Naunyn — Schmiedeberg's Arch. Pharmacol. 225:278–284.
Shain, W. G., and Martin, D. L. 1984. Activation of β-adrenergic receptors stimulates taurine release from glial cells. Cell. Mol. Neurobiol. 4:191–196.
Sharma, S. K., Klee, W. A., and Nitrenberg, M. 1975. Dual regulation of adenylate cyclase accounts for narcotic dependence and tolerance. Proc. Natl. Acad. Sci. 72:3092–3096.
Steiner, A. L., Pagliara, A. S., Chase, L. R., and Kipnis, D. M. 1972. Radioimmunoassay for cyclic nucleotides. II. Adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate in mammalian tissues and body fluids. J. Biol. Chem. 247:1114–1120.
Steiner, A. L., Parker, C. W., and Kipnis, D. M. 1972. Radioimmunoassay for cyclic nucleotides. I. Preparation of antibodies and iodinated cyclic nucleotides. J. Biol. Chem. 247:1106–1113.
Wohltmann, M., Roth, B. L., and Coscia, C. J. 1982. Differential postnatal development of mu and delta opiate receptors. Dev. Brain Res. 3:679–684.
Wu, D. K., and deVellis, J. 1983. Effect of forskolin on primary cultures of astrocytes and oligodendrocytes. J. Cycl. Nucl. Prot. Phosph. Res. 9:59–67.
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Eriksson, P.S., Hansson, E. & Rönnbäck, L. δ and κ Opiate receptors in primary astroglial cultures from rat cerebral cortex. Neurochem Res 15, 1123–1126 (1990). https://doi.org/10.1007/BF01101714
- primary culture